【摘要】：目的：基于腸功能異常的便秘動物模型,探究針刺腸的合穴、募穴、合募穴調(diào)節(jié)腸功能的效應(yīng)差異,觀察電針干預(yù)前后腸神經(jīng)抑制性運動神經(jīng)元的變化情況,分析電針不同穴位調(diào)節(jié)腸功能效應(yīng)差異與腸抑制性運動神經(jīng)元的關(guān)系,為臨床合理選取腧穴治療腸腑病癥提供參考。方法：Sprague-Dawley雄性大鼠4周齡110只,隨機分為正常組20只,造模組90只,在造模14天期間給予造模組0-4℃冰水灌胃,并觀察大鼠糞便的濕重、干重、首粒排便時間與小腸推進功能。造模結(jié)束后第15天,挑選造模成功的大鼠隨機分為模型組12只,電針干預(yù)三組(電針募穴、電針合穴、電針合募穴組各6只),電針強度1.5mA,電針頻率2/15HZ,每只大鼠電針干預(yù)15min/天,電針期間觀察大鼠糞便的濕重、干重、首粒排便時間進行比較分析,于實驗周期第22天將正常組、模型組與電針三組大鼠結(jié)腸組織取材并固定,以免疫組化測定近端結(jié)腸與遠端結(jié)腸肌間神經(jīng)叢nNOS陽性反應(yīng)神經(jīng)元平均光密度,進行半定量分析。結(jié)果：(1)在造模期間,模型組大鼠糞便濕重、干重均減少,糞便濕重在第2,8,12,14天較正常組比較降低(p0.05),糞便干重在第10,12,14天低于正常組(p0.05),首粒排便時間低于正常組(p0.05)。(2)在電針干預(yù)期間,電針三組穴位均能夠使糞便濕重向正常水平恢復(fù),電針合募穴優(yōu)于電針募穴與電針合穴(p0.05)。電針三組穴位均有使糞便干重向正常水平恢復(fù)的作用。電針三組穴位對首粒排便時間均有改善,電針募穴有使結(jié)腸功能向正常水平恢復(fù)的趨勢,但并不能夠使其恢復(fù)至正常水平,電針募穴對結(jié)腸功能的改善弱于電針合穴與電針合募穴。(3)在近端結(jié)腸肌間神經(jīng)叢,模型組nNOS陽性神經(jīng)元顯著高于正常組(p0.01)。電針干預(yù)治療后,三組穴位電針治療均能使便秘大鼠近端結(jié)腸肌間神經(jīng)叢異常升高的nNOS明顯降低(p0.01),且電針合穴與電針合募穴均能夠nNOS恢復(fù)到正常水平,而電針募穴沒有出現(xiàn)這一現(xiàn)象,且電針募穴與電針合募穴比較差異有統(tǒng)計學(xué)意義(p0.01)。(4)在遠端結(jié)腸肌間神經(jīng)叢,模型組nNOS明顯高于正常組(p0.01),電針三組穴位均能使異常升高的nNOS降低(p0.01),且電針合穴與電針合募穴能夠使nNOS恢復(fù)至正常水平,但電針募穴并不能使其恢復(fù)至正常水平(p0.01),綜上說明遠端結(jié)腸肌間神經(jīng)叢nNOS在電針合穴、電針合募穴調(diào)節(jié)便秘大鼠腸功能中的作用更加明顯。結(jié)論：(1)本實驗再次驗證了冰水灌胃復(fù)制便秘模型大鼠的可行性與優(yōu)點,即該造模方法能夠使得整個實驗周期簡短,更易于操作。近、遠端結(jié)腸肌間神經(jīng)叢抑制性神經(jīng)元nNOS的異常升高是導(dǎo)致便秘發(fā)生的局部神經(jīng)機制之一。(2)電針合穴、募穴及合募穴均能有效改善腸功能,使腸功能向正常水平恢復(fù)。本研究顯示在便秘狀態(tài)下,兩集元穴的配穴作用大于單穴的效應(yīng)。電針合募穴對便秘大鼠結(jié)腸運動的促進作用,可能骶副交感神經(jīng)更容易被激活從而產(chǎn)生更優(yōu)的效應(yīng),才會使得兩集元穴的配伍效應(yīng)加強。(3)電針募穴調(diào)節(jié)近、遠端結(jié)腸肌間神經(jīng)叢抑制性神經(jīng)元nNOS的作用較弱,電針合募穴的作用最優(yōu)。電針合募穴調(diào)節(jié)腸功能的效應(yīng)存在差異,近、遠端神經(jīng)肌間神經(jīng)叢神經(jīng)元nNOS的的不同改變可能是基礎(chǔ)。
[Abstract]:Objective: Based on the constipation animal model of intestinal dysfunction, to explore the difference of regulating intestinal function by acupuncture at Hexue, Muxu and HeMu points, to observe the changes of intestinal nerve inhibitory motor neurons before and after electroacupuncture intervention, and to analyze the relationship between the difference of regulating intestinal function by Electroacupuncture at different acupoints and intestinal inhibitory motor neurons. Methods: 110 Sprague-Dawley male rats aged 4 weeks were randomly divided into the normal group (20 rats) and the model group (90 rats). During the 14 days of modeling, the rats were given ice water at 0-4, and the wet weight, dry weight, first defecation time and small intestinal propulsion function were observed. Twelve rats were randomly divided into three groups: electro-acupuncture group, electro-acupuncture group, electro-acupuncture group and electro-acupuncture group. The intensity of electro-acupuncture was 1.5mA and the frequency of electro-acupuncture was 2/15HZ. Each rat was intervened by electro-acupuncture for 15 min/day. The average optical density of nNOS-positive neurons in the proximal and distal colonic myenteric plexus was determined by immunohistochemistry and semi-quantitative analysis was made. Results: (1) During the modeling period, the wet and dry weight of feces in the model group decreased, and the wet weight of feces in the proximal and distal colonic myenteric plexus decreased. The dry weight of feces on the 10th, 12th and 14th day was lower than that of the normal group (p0.05), and the first defecation time was lower than that of the normal group (p0.05). Electro-acupuncture at three acupoints can improve the time of first bowel movement. Electro-acupuncture at the acupoints has a tendency to restore colonic function to normal level, but can not restore it to normal level. The improvement of colonic function by electro-acupuncture at the acupoints is weaker than that by electro-acupuncture at the acupoints and electro-acupuncture at the acupoints. The nNOS positive neurons in the nerve plexus and the model group were significantly higher than those in the normal group (p0.01). After electroacupuncture treatment, the abnormal increase of nNOS in the proximal colonic myenteric plexus in the constipated rats was significantly decreased by Electroacupuncture at all three acupoints (p0.01), and both electroacupuncture and electroacupuncture at the same acupoint could restore the nNOS to the normal level, but electroacupuncture at the same acupoint did not appear. (4) in the distal colonic myenteric plexus, the nNOS in the model group was significantly higher than that in the normal group (p0.01). electroacupuncture at all three acupoints could reduce the abnormally elevated nNOS (p0.01), and electroacupuncture at both acupoints could restore nNOS to normal level, but electroacupuncture at all three acupoints could restore nNOS to normal level. In conclusion: (1) This experiment once again verified the feasibility and advantages of cold water gastric perfusion in duplicating constipation model rats, that is, this method can make the model. Nearly, the abnormal elevation of nNOS in the distal colonic myenteric plexus inhibitory neurons is one of the local neural mechanisms leading to constipation. (2) Electroacupuncture at Hexue, Muxu and HeMu can effectively improve intestinal function and restore intestinal function to normal level. The effect of acupoints matching is greater than that of single acupoint. The promoting effect of electroacupuncture combined with Mu acupoint on colonic motility in constipated rats may be that the sacral parasympathetic nerve is more easily activated to produce a better effect, so that the compatibility effect of the two Jiyuan acupoints can be strengthened. (3) The effect of electroacupuncture combined with Mu acupoint matching on the inhibition neuron nNOS of distal colonic myenteric plexus near to Electroacupuncture combined with Mu acupoint has different effects on regulating intestinal function, and the different changes of nNOS in distal neuromuscular plexus neurons may be the basis.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245

【參考文獻】

相關(guān)期刊論文 前1條

1 Gabrio Bassotti;Vincenzo Villanacci;;Slow transit constipation: A functional disorder becomes an enteric neuropathy[J];World Journal of Gastroenterology;2006年29期

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本文編號：2198382