【摘要】：目的:觀察脾虛痰濁動(dòng)脈硬化(AS)巴馬小型豬水液代謝的變化及益氣健脾、祛痰化瘀法的作用。方法:將20頭巴馬小型豬隨機(jī)分組,正常組、模型組、益氣健脾組及益氣健脾祛痰化瘀組各5頭。以高脂飲食、跑步勞倦結(jié)合冠脈球囊拉傷法制備脾虛痰濁AS病證結(jié)合模型至24周。正常組巴馬小型豬喂正常飼料,模型組巴馬小豬行冠脈球囊拉傷后予高脂飼料結(jié)合跑步過(guò)勞制備脾虛痰濁AS模型。益氣健脾組及益氣健脾、祛痰化瘀組在模型組制備基礎(chǔ)上,于高脂飼料中添加相應(yīng)的中藥顆粒劑。觀察48周后各組巴馬小豬的血清鉀、鈉、氯、尿素氮、肌酐的含量,血清水液代謝調(diào)節(jié)激素精氨酸加壓素(AVP)、心房利鈉肽(ANP)、醛固酮(ALD)以及腎組織水通道蛋白(AQP)1、2、3、4、鈉鉀ATP酶蛋白(Na~+-K~+-ATPase)表達(dá)變化。結(jié)果:與正常組相比,模型組血清鉀、鈉、氯、尿素氮、肌酐水平無(wú)變化(P0.05),ANP、AVP上調(diào)(P0.05),ALD有上調(diào)趨勢(shì)(P0.05),模型組腎組織AQP1、2、3及鈉鉀ATP酶蛋白表達(dá)下調(diào)(P0.05),AQP4表達(dá)上調(diào)。與模型組比較,益氣健脾、祛痰化瘀組ANP下調(diào)(P0.05),AVP、ALD下調(diào)(P0.05),益氣健脾組AVP下調(diào)(P0.05);益氣健脾組及益氣健脾、祛痰化瘀組腎組織AQP1、2、3表達(dá)均上調(diào)(P0.05),AQP4表達(dá)下調(diào);益氣健脾組鈉鉀ATP酶蛋白表達(dá)上調(diào)(P0.05)。結(jié)論:脾虛痰濁AS模型未見(jiàn)血清離子紊亂,但水液代謝調(diào)節(jié)激素中ANP、AVP、ALD有上調(diào),腎組織AQP1、2、3下調(diào),下調(diào),AQP4表達(dá)上調(diào);益氣健脾及益氣健脾祛痰化瘀法可上調(diào)腎組織AQP1、2、3,下調(diào)AQP4。益氣健脾祛痰化瘀法可下調(diào)水液代謝調(diào)節(jié)激素,益氣健脾法可上調(diào)鈉鉀ATP酶。益氣健脾、祛痰化瘀法在恢復(fù)水液代謝調(diào)節(jié)激素方面優(yōu)于益氣健脾組。
[Abstract]:Objective: to observe the changes of water and liquid metabolism in (AS) small pig with spleen deficiency and phlegm turbid arteriosclerosis and the effect of Qi tonifying spleen removing phlegm and removing blood stasis. Methods: 20 Bama miniature pigs were randomly divided into normal group, model group, invigorating qi group and invigorating spleen group and removing phlegm and removing blood stasis group. The combined model of spleen deficiency and phlegm turbid as disease was established by high fat diet, fatigue of running and balloon injury of coronary artery for 24 weeks. The normal diet was fed to the normal Bama miniature pigs and the model group was fed with high fat diet combined with running overstrain to establish the model of as with spleen deficiency and phlegm turbidity after coronary balloon injury. On the basis of the preparation of the model group, the groups of invigorating qi and invigorating spleen, expelling phlegm and removing blood stasis, added the corresponding traditional Chinese medicine granules in high fat diet. After 48 weeks, serum potassium, sodium, chlorine, urea nitrogen and creatinine were observed in each group of Bama piglets. Serum water solution metabolism regulated the expression of arginine vasopressin (AVP), atrial natriuretic peptide (ANP),) aldosterone (ALD) and renal tissue aquaporin (AQP) (AQP) 1), ATP protein (Na ~ -K ~ -ATPase). Results: compared with normal group, serum potassium, sodium, chlorine, urea nitrogen and creatinine levels in model group showed no change (P0.05). Compared with the model group, ANP was down-regulated (P0.05) and AVP was down-regulated (P0.05) in Qi-invigorating spleen group and dispelling phlegm and removing blood stasis group (P0.05), and the expression of AQP1O2P4 in kidney tissue was down-regulated (P0.05) in invigorating qi group, invigorating qi and invigorating spleen group, dispelling phlegm and removing blood stasis group (P0.05), and down-regulation of AQP4 expression in kidney tissue of Qi-invigorating group and invigorating spleen group (P0.05). The expression of sodium and potassium ATP enzyme protein was up-regulated in invigorating spleen group (P0.05). Conclusion: no serum ion disturbance was found in the model of as with spleen deficiency and phlegm turbidity, but the ALD of ANPP AVPN in water and liquid metabolism regulatory hormone was up-regulated, the expression of AQP4 was down-regulated in renal tissue by AQP1, and the expression of AQP4 was down-regulated by the method of invigorating qi and invigorating spleen and removing phlegm and removing blood stasis in renal tissue. The method of invigorating qi and invigorating spleen and removing phlegm and removing blood stasis can down-regulate the metabolism regulating hormone of water and liquid, and replenish qi and invigorate spleen can upregulate ATP enzyme of sodium and potassium The method of invigorating qi and invigorating spleen and removing phlegm and removing blood stasis is superior to the group of replenishing qi and invigorating spleen.
【作者單位】： 遼寧中醫(yī)藥大學(xué);遼寧中醫(yī)藥大學(xué)中醫(yī)臟象理論及應(yīng)用教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家教育部重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金項(xiàng)目(ZYZX1515) 遼寧省中醫(yī)外治心血管系統(tǒng)疾病轉(zhuǎn)化醫(yī)學(xué)研究中心建設(shè)項(xiàng)目(2014225004)
【分類號(hào)】：R259

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