本文選題：雄激素合成酶 + 證候�。� 參考：《中國(guó)中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志》2017年10期

【摘要】：目的:研究睪丸雄激素合成酶與腫瘤狀態(tài)下小鼠證候的關(guān)系。方法:采用實(shí)驗(yàn)小鼠及其標(biāo)準(zhǔn)化四診與辨證方法篩選H22荷瘤小鼠邪毒壅盛證、氣虛證、陽(yáng)氣虛證、氣陰陽(yáng)虛證,并以Gene Chip Mouse Exon 1.0 ST Array技術(shù)檢測(cè)睪丸組織基因芯片表達(dá)譜,重點(diǎn)分析睪丸基因芯片中雄激素合成酶及其調(diào)節(jié)因子在不同證候小鼠中的差異表達(dá)。結(jié)果:與正常組比較,Star、Stard5、Cyp17a1、Hsd3b6、Hsd17b3、Sult1e1等基因在早期邪毒壅盛證和氣虛證小鼠呈現(xiàn)下調(diào)趨勢(shì),且在中晚期陽(yáng)氣虛證和氣陰陽(yáng)虛證下調(diào)幅度更加顯著,Cyp11a1、Hsd3b1、Hsd17b10、Hsd17b11、Hsd17b12、Fdx1、Fdxr、Por、Sult1a1等僅在中晚期陽(yáng)氣虛證和氣陰陽(yáng)虛證中顯著下調(diào),Cyp17a1僅在睪丸組織特異性中表達(dá)。結(jié)論:H22荷瘤小鼠腫瘤形成后,自發(fā)產(chǎn)生的虛證越嚴(yán)重,其睪丸雄激素合成酶基因轉(zhuǎn)錄活性越低。
[Abstract]:Objective: to study the relationship between testis androgen synthase and syndromes in mice with tumor. Methods: the experimental mice and their four standardized diagnosis and syndrome differentiation methods were used to screen the syndrome of accumulation of evil toxin, deficiency of qi, deficiency of yang and deficiency of qi yin and yang in H22 tumor-bearing mice. Gene Chip Mouse Exon 1.0ST Array technique was used to detect the gene chip expression profile of testis tissue. The differential expression of androgen synthase and its regulatory factors in testicular microarray in mice with different syndromes was analyzed. Results: compared with the normal group, the genes such as Cyp17a1, Hsd3b6, Hsd17b3, Sult1e1 and so on showed a down-regulation trend in the early stage of the mice with the syndrome of excessive accumulation of pathogenic toxin and deficiency of qi. Moreover, the down-regulation of Cyp11a1Hsd3b1C Hsd17b10, Hsd17b11, Hsd17b12, Fdx1FdxrLi, Fdx1FdxrLi, Fdx1a1 and Qi-Yin Yang deficiency syndrome was significantly down-regulated only in testicular tissue specificity in the middle and late stage of Yang Qi deficiency syndrome and Qi Yin and Yang deficiency syndrome, and the down-regulation of Cyp17a1 was only found in testicular tissue. Conclusion the more serious the spontaneous deficiency syndrome is, the lower the transcriptional activity of androgen synthase gene in testis is.
【作者單位】： 上海中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：上海市科委國(guó)際合作資助項(xiàng)目(064307052)-中醫(yī)藥有關(guān)腫瘤辨證論治機(jī)制的系統(tǒng)生物學(xué)研究 上海市進(jìn)一步加快中醫(yī)藥事業(yè)發(fā)展三年行動(dòng)計(jì)劃(2014~2016年)(ZY3-CCCX-3-3010)-溫腎壯陽(yáng)中藥有效成分的類固醇激素樣效應(yīng)比較及其相須配伍的物質(zhì)基礎(chǔ)
【分類號(hào)】：R273
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本文編號(hào)：2083525