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  本文選題：盆腔炎性疾病后遺癥 + 正虛邪戀�。� 參考：《黑龍江中醫(yī)藥大學》2017年碩士論文

【摘要】：目的:通過觀察針刺結合盆舒飲對盆腔炎性疾病后遺癥大鼠血清中促炎性細胞因子IL-6、IL-8的含量影響,探討其治療盆腔炎性疾病后遺癥的作用機制及效果優(yōu)勢。方法:1、選用Wistar雌性大鼠作為受試對象,采用"機械損傷+混合菌感染"的方法建立盆腔炎性疾病后遺癥大鼠模型。分別于造模后第7、14、21天對大鼠子宮組織進行病理學觀察。2、隨機抽取8只大鼠作為空白組,將其余大鼠進行造模,造模后的大鼠隨機分成5組,于造模后第15天進行干預治療�？瞻讓φ战M和模型組都給予蒸餾水灌胃,針刺組選取腧穴關元、血海和三陰交進行治療,中成藥組以丹黃去瘀膠囊懸液灌胃,中藥組以盆舒飲湯劑進行灌胃、針藥結合組大鼠給予針刺刺激同時聯(lián)合盆舒飲灌胃治療,14天后,采用HE染色法,通過光鏡對大鼠子宮組織病理改變進行觀察;用ELISA法檢測大鼠血清中的促炎性因子IL-6、IL-8的濃度。結果:1、成功建立盆腔炎性疾病后遺癥大鼠模型。通過觀察發(fā)現(xiàn):造模術后7天,大鼠子宮組織明顯充血,炎細胞浸潤,無盆腔粘連、纖維化改變等典型慢性炎癥表現(xiàn);造模術后14天,肉眼觀察大鼠盆腔組織廣泛粘連,子宮積水嚴重,光鏡下可見腺體擴張,纖維化改變明顯;IL-6、IL-8在SPID模型大鼠血清中的表達明顯高于正常大鼠,p0.01。2、經(jīng)過兩周干預治療后,各治療組大鼠盆腔情況均有不同程度好轉(zhuǎn),其中以針刺結合盆舒飲組改善程度最佳;各治療組大鼠血清中IL-6、IL-8水平均有所下調(diào),其中以針刺聯(lián)合盆舒飲組下降最明顯,與正常對照組比較,p0.05。結論:通過"機械損傷+混合菌感染"的方法可成功建立SPID大鼠模型。針刺聯(lián)合盆舒飲主要通過抑制炎癥大鼠血清中的促炎性細胞因子IL-6、IL-8的表達,來減輕組織損傷,促進炎癥吸收,從而達到治療盆腔炎性疾病后遺癥的目的。
[Abstract]:Objective: To observe the effect of acupuncture combined with basin Shu Yin on the serum levels of proinflammatory cytokines IL-6 and IL-8 in the serum of the sequelae of pelvic inflammatory disease in rats, and to explore the mechanism and effect of its effect on the sequelae of pelvic inflammatory disease. Methods: 1, the female rats of Wistar were selected as subjects, and "mechanical injury + mixed bacteria infection" was used. The rat model of the sequelae of pelvic inflammatory disease was established by the method..2 was observed on the uterus tissue of the rats on day 7,14,21 after the model. 8 rats were randomly selected as the blank group. The other rats were made into the model. The rats were randomly divided into 5 groups, and the intervention treatment was carried out at fifteenth days after the model. The blank control group and the model group were all given. The acupuncture group was given the stomach with distilled water, the acupuncture group selected acupoint Guanyuan, Xuehai and Sanyinjiao for treatment. The Chinese patent medicine group was filled with Dan Huang Qi stasis capsule suspension, and the Chinese medicine group was treated with basin Shu Yin decoction. The acupuncture and drug combination group was given acupuncture stimulation at the same time with basin Shu Yin to fill the stomach. After 14 days, the uterus tissue of rats was used by the HE staining method. The pathological changes were observed and the concentration of proinflammatory factor IL-6 and IL-8 in the serum of rats was detected by ELISA. Results: 1, the rat model of the sequelae of pelvic inflammatory disease was successfully established. Through observation, it was found that the uterus tissue was obviously congested, inflammatory cell infiltration, no pelvic adhesions, fibrosis changes and other typical chronic inflammatory manifestations in the rat uterus on the 7 day after the operation. 14 days after the operation, the pelvic tissue was widely adhered by the naked eye, the hydrops of the uterus were serious, the glandular expansion was seen under the light microscope, and the fibrosis changes were obvious. The expression of IL-6, IL-8 in the serum of SPID model rats was significantly higher than that of the normal rats, p0.01.2. After two weeks of intervention, the pelvic cavity of the rats in each treatment group improved in varying degrees. The improvement of the combination of acupuncture combined with basin Shu Shu group was the best. The average decrease of IL-6 and IL-8 water in the serum of the rats in each treatment group was down, with the most obvious decrease in the acupuncture combined with the basin Shu Shu group, and compared with the normal control group. P0.05. conclusion: the SPID rat model could be successfully established through the method of "mechanical injury + mixed bacteria infection". The expression of pro-inflammatory cytokine IL-6 and IL-8 in the serum of inflammatory rats can reduce tissue damage and promote inflammatory absorption, thus achieving the purpose of treating the sequelae of pelvic inflammatory disease.
【學位授予單位】：黑龍江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R271.9

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