本文選題：盆腔炎性疾病后遺癥 + 正虛邪戀　； 參考：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:通過(guò)觀察針刺結(jié)合盆舒飲對(duì)盆腔炎性疾病后遺癥大鼠血清中促炎性細(xì)胞因子IL-6、IL-8的含量影響,探討其治療盆腔炎性疾病后遺癥的作用機(jī)制及效果優(yōu)勢(shì)。方法:1、選用Wistar雌性大鼠作為受試對(duì)象,采用"機(jī)械損傷+混合菌感染"的方法建立盆腔炎性疾病后遺癥大鼠模型。分別于造模后第7、14、21天對(duì)大鼠子宮組織進(jìn)行病理學(xué)觀察。2、隨機(jī)抽取8只大鼠作為空白組,將其余大鼠進(jìn)行造模,造模后的大鼠隨機(jī)分成5組,于造模后第15天進(jìn)行干預(yù)治療�？瞻讓�(duì)照組和模型組都給予蒸餾水灌胃,針刺組選取腧穴關(guān)元、血海和三陰交進(jìn)行治療,中成藥組以丹黃去瘀膠囊懸液灌胃,中藥組以盆舒飲湯劑進(jìn)行灌胃、針?biāo)幗Y(jié)合組大鼠給予針刺刺激同時(shí)聯(lián)合盆舒飲灌胃治療,14天后,采用HE染色法,通過(guò)光鏡對(duì)大鼠子宮組織病理改變進(jìn)行觀察;用ELISA法檢測(cè)大鼠血清中的促炎性因子IL-6、IL-8的濃度。結(jié)果:1、成功建立盆腔炎性疾病后遺癥大鼠模型。通過(guò)觀察發(fā)現(xiàn):造模術(shù)后7天,大鼠子宮組織明顯充血,炎細(xì)胞浸潤(rùn),無(wú)盆腔粘連、纖維化改變等典型慢性炎癥表現(xiàn);造模術(shù)后14天,肉眼觀察大鼠盆腔組織廣泛粘連,子宮積水嚴(yán)重,光鏡下可見(jiàn)腺體擴(kuò)張,纖維化改變明顯;IL-6、IL-8在SPID模型大鼠血清中的表達(dá)明顯高于正常大鼠,p0.01。2、經(jīng)過(guò)兩周干預(yù)治療后,各治療組大鼠盆腔情況均有不同程度好轉(zhuǎn),其中以針刺結(jié)合盆舒飲組改善程度最佳;各治療組大鼠血清中IL-6、IL-8水平均有所下調(diào),其中以針刺聯(lián)合盆舒飲組下降最明顯,與正常對(duì)照組比較,p0.05。結(jié)論:通過(guò)"機(jī)械損傷+混合菌感染"的方法可成功建立SPID大鼠模型。針刺聯(lián)合盆舒飲主要通過(guò)抑制炎癥大鼠血清中的促炎性細(xì)胞因子IL-6、IL-8的表達(dá),來(lái)減輕組織損傷,促進(jìn)炎癥吸收,從而達(dá)到治療盆腔炎性疾病后遺癥的目的。
[Abstract]:Objective: to observe the effect of acupuncture combined with Pan-Shuyin on the content of IL-6 and IL-8 in the serum of rats with pelvic inflammatory disease sequelae, and to explore the mechanism and advantages of the treatment of pelvic inflammatory disease sequelae. Methods the female Wistar rats were selected as subjects, and the model of pelvic inflammatory disease sequelae was established by the method of "mechanical injury mixed bacteria infection". The uterine tissues of rats were observed by pathological observation on day 7, 14, 21, respectively. 8 rats were randomly selected as blank group. The other rats were randomly divided into 5 groups, and the rats were treated with intervention on the 15th day after the model. The blank control group and the model group were given distilled water, the acupuncture group was treated with acupoint Guanyuan, Xuehai and Sanyinjiao, the traditional Chinese medicine group was perfused with Danhuangzaiyu capsule suspension, and the traditional Chinese medicine group was perfused with Pan-Shuyin decoction. After 14 days of acupuncture stimulation combined with gastric perfusion, the pathological changes of rat uterus were observed by HE staining, and the concentration of IL-6 and IL-8 in serum of rats was detected by Elisa. Results the rat model of pelvic inflammatory disease sequelae was established successfully. It was found that there were obvious hyperemia, inflammatory cell infiltration, no pelvic adhesion, fibrosis and other typical chronic inflammatory manifestations in rat uterus 7 days after modeling, and extensive adhesions of pelvic tissue were observed with naked eye on 14 days after model making. The uterine hydronephrosis was serious, glandular dilatation was observed under light microscope, and the expression of IL-6 IL-8 in serum of SPID model rats was significantly higher than that of normal rats. After two weeks of intervention, the pelvic conditions of each treatment group were improved to some extent. The level of IL-6 and IL-8 in serum of rats in each treatment group was down-regulated, especially in the acupuncture combined with Pan-Shuyin group, which was significantly lower than that in the normal control group (P 0.05). Conclusion: SPID rat model can be established successfully by the method of "mixed strain of mechanical injury". Acupuncture combined with Pan-Shuyin can reduce tissue injury and promote inflammatory absorption by inhibiting the expression of IL-6 and IL-8 in the serum of inflammatory rats so as to achieve the purpose of treating the sequelae of pelvic inflammatory disease.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R271.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張嘉妮;戴冰;李玉星;楊夢(mèng)琳;肖子曾;石繼連;;酒制山茱萸炮制研究進(jìn)展[J];中國(guó)現(xiàn)代應(yīng)用藥學(xué);2016年12期

2 李本科;;針刺對(duì)女性低度炎癥性慢性盆腔炎臨床癥狀與炎性因子的影響[J];中外醫(yī)療;2016年11期

3 岳美穎;潘媛;敖慧;;白術(shù)化學(xué)、藥理與臨床研究進(jìn)展[J];亞太傳統(tǒng)醫(yī)藥;2016年05期

4 歐劍英;;中藥離子導(dǎo)入治療慢性盆腔炎的觀察與護(hù)理[J];內(nèi)蒙古中醫(yī)藥;2015年12期

5 楊紅英;梁艷;;保婦康栓聯(lián)合頭孢曲松鈉、甲硝唑治療慢性盆腔炎的臨床研究[J];現(xiàn)代藥物與臨床;2015年06期

6 宋玉娟;張殿全;蘇丹萍;;腹針配合中藥治療氣滯血瘀型慢性盆腔痛的臨床研究[J];上海針灸雜志;2015年05期

7 胡艷玲;張潔;曹曉娟;王曉麗;武捷;;中藥離子導(dǎo)入治療慢性盆腔炎的效果觀察[J];實(shí)用婦科內(nèi)分泌電子雜志;2015年05期

8 薛翔;劉紅梅;邵旦兵;唐文杰;聶時(shí)南;;JAK/STAT信號(hào)通路調(diào)節(jié)機(jī)制的研究進(jìn)展[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2015年11期

9 陸小華;馬驍;王建;朱云;周宗元;陳哲;趙艷玲;王伽伯;;赤芍的化學(xué)成分和藥理作用研究進(jìn)展[J];中草藥;2015年04期

10 武宏燕;鄭鑫;惠雪蓮;張曉峰;;觀察補(bǔ)腎活血法聯(lián)合中藥保留灌腸治療盆腔炎性疾病后遺癥的臨床療效[J];中國(guó)實(shí)用醫(yī)藥;2014年33期

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