本文選題：盆腔炎性疾病后遺癥 + 正虛邪戀��； 參考：《黑龍江中醫(yī)藥大學》2017年碩士論文

【摘要】：目的:通過觀察針刺結合盆舒飲對盆腔炎性疾病后遺癥大鼠血清中促炎性細胞因子IL-6、IL-8的含量影響,探討其治療盆腔炎性疾病后遺癥的作用機制及效果優(yōu)勢。方法:1、選用Wistar雌性大鼠作為受試對象,采用"機械損傷+混合菌感染"的方法建立盆腔炎性疾病后遺癥大鼠模型。分別于造模后第7、14、21天對大鼠子宮組織進行病理學觀察。2、隨機抽取8只大鼠作為空白組,將其余大鼠進行造模,造模后的大鼠隨機分成5組,于造模后第15天進行干預治療�？瞻讓φ战M和模型組都給予蒸餾水灌胃,針刺組選取腧穴關元、血海和三陰交進行治療,中成藥組以丹黃去瘀膠囊懸液灌胃,中藥組以盆舒飲湯劑進行灌胃、針藥結合組大鼠給予針刺刺激同時聯(lián)合盆舒飲灌胃治療,14天后,采用HE染色法,通過光鏡對大鼠子宮組織病理改變進行觀察;用ELISA法檢測大鼠血清中的促炎性因子IL-6、IL-8的濃度。結果:1、成功建立盆腔炎性疾病后遺癥大鼠模型。通過觀察發(fā)現(xiàn):造模術后7天,大鼠子宮組織明顯充血,炎細胞浸潤,無盆腔粘連、纖維化改變等典型慢性炎癥表現(xiàn);造模術后14天,肉眼觀察大鼠盆腔組織廣泛粘連,子宮積水嚴重,光鏡下可見腺體擴張,纖維化改變明顯;IL-6、IL-8在SPID模型大鼠血清中的表達明顯高于正常大鼠,p0.01。2、經過兩周干預治療后,各治療組大鼠盆腔情況均有不同程度好轉,其中以針刺結合盆舒飲組改善程度最佳;各治療組大鼠血清中IL-6、IL-8水平均有所下調,其中以針刺聯(lián)合盆舒飲組下降最明顯,與正常對照組比較,p0.05。結論:通過"機械損傷+混合菌感染"的方法可成功建立SPID大鼠模型。針刺聯(lián)合盆舒飲主要通過抑制炎癥大鼠血清中的促炎性細胞因子IL-6、IL-8的表達,來減輕組織損傷,促進炎癥吸收,從而達到治療盆腔炎性疾病后遺癥的目的。
[Abstract]:Objective: to observe the effect of acupuncture combined with Pan-Shuyin on the content of IL-6 and IL-8 in the serum of rats with pelvic inflammatory disease sequelae, and to explore the mechanism and advantages of the treatment of pelvic inflammatory disease sequelae. Methods the female Wistar rats were selected as subjects, and the model of pelvic inflammatory disease sequelae was established by the method of "mechanical injury mixed bacteria infection". The uterine tissues of rats were observed by pathological observation on day 7, 14, 21, respectively. 8 rats were randomly selected as blank group. The other rats were randomly divided into 5 groups, and the rats were treated with intervention on the 15th day after the model. The blank control group and the model group were given distilled water, the acupuncture group was treated with acupoint Guanyuan, Xuehai and Sanyinjiao, the traditional Chinese medicine group was perfused with Danhuangzaiyu capsule suspension, and the traditional Chinese medicine group was perfused with Pan-Shuyin decoction. After 14 days of acupuncture stimulation combined with gastric perfusion, the pathological changes of rat uterus were observed by HE staining, and the concentration of IL-6 and IL-8 in serum of rats was detected by Elisa. Results the rat model of pelvic inflammatory disease sequelae was established successfully. It was found that there were obvious hyperemia, inflammatory cell infiltration, no pelvic adhesion, fibrosis and other typical chronic inflammatory manifestations in rat uterus 7 days after modeling, and extensive adhesions of pelvic tissue were observed with naked eye on 14 days after model making. The uterine hydronephrosis was serious, glandular dilatation was observed under light microscope, and the expression of IL-6 IL-8 in serum of SPID model rats was significantly higher than that of normal rats. After two weeks of intervention, the pelvic conditions of each treatment group were improved to some extent. The level of IL-6 and IL-8 in serum of rats in each treatment group was down-regulated, especially in the acupuncture combined with Pan-Shuyin group, which was significantly lower than that in the normal control group (P 0.05). Conclusion: SPID rat model can be established successfully by the method of "mixed strain of mechanical injury". Acupuncture combined with Pan-Shuyin can reduce tissue injury and promote inflammatory absorption by inhibiting the expression of IL-6 and IL-8 in the serum of inflammatory rats so as to achieve the purpose of treating the sequelae of pelvic inflammatory disease.
【學位授予單位】：黑龍江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R271.9

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