本文選題：益氣血法 + 經(jīng)后增殖方��； 參考：《實(shí)用醫(yī)學(xué)雜志》2017年13期

【摘要】：目的觀察經(jīng)后增殖方對(duì)超排卵小鼠卵巢凋亡相關(guān)基因及其蛋白表達(dá)的調(diào)節(jié)作用,探討中藥益氣血法對(duì)超排卵小鼠卵巢顆粒細(xì)胞凋亡及卵泡質(zhì)量的影響。方法建立超排卵小鼠模型,30只小鼠隨機(jī)分為空白組、模型組、中藥治療組。采用實(shí)時(shí)熒光定量PCR(real-time quantitative PCR,qPCR)和免疫印跡法(Western blot)分別檢測(cè)Bcl-2、Bax、Caspase3基因和蛋白的表達(dá)水平。結(jié)果中藥治療組Bax、Cas-pase3 mRNA的表達(dá)水平與模型組比較(0.895 vs 1.779,0.674 vs 2.066,均P0.05),差異均有統(tǒng)計(jì)學(xué)意義;中藥治療組Bcl-2、Bax、Caspase3 mRNA的表達(dá)水平與空白組比較(1.11 vs 1.26,0.89 vs 0.79,0.67 vs 0.96),差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);中藥治療組Bcl-2、Bax、Caspase3蛋白的表達(dá)水平與模型組比較(0.62 vs0.41,0.79 vs 1.06,0.49 vs 0.89),差異均有統(tǒng)計(jì)學(xué)意義(P0.05),與空白組比較(0.62 vs 0.64,0.79 vs 0.73,0.49 vs 0.50)差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論經(jīng)后增殖方能促進(jìn)Bcl-2蛋白的表達(dá),抑制Bax、Cas-pase3基因及蛋白的表達(dá),抑制超排卵小鼠卵巢顆粒細(xì)胞凋亡至接近自然排卵小鼠的水平,提高超排卵小鼠卵泡質(zhì)量。
[Abstract]:Objective to observe the effect of Jinghou recipe on the expression of ovarian apoptosis-related genes and proteins in superovulation mice, and to explore the effect of Yiqi and Xue method on ovarian granulosa cell apoptosis and follicle quality in superovulation mice. Methods Thirty mice with hyperovulation were randomly divided into blank group, model group and traditional Chinese medicine treatment group. The expression of caspase3 gene and protein were detected by real-time quantitative qPCR and Western blotting, respectively. Results compared with the model group, the expression level of Bax-Caspase3 mRNA in the traditional Chinese medicine treatment group was 0.895 vs 1.779c0.674 vs 2.066, all the differences were statistically significant (P 0.05). The expression level of Bcl-2OBax-Caspase3 mRNA in the TCM treatment group was higher than that in the control group (1.11 vs 1.260.89 vs 0.79U 0.67 vs 0.96), and there was no significant difference in the expression of Bcl-2Bax-Caspase3 protein between the Chinese medicine group and the model group (0.62 vs 0.41 vs 1.06 鹵0.49 vs 0.89, respectively), and the difference was statistically significant (P 0.05P 0.05A). The expression of Bcl-2AXCaspase3 mRNA in the treatment group was significantly higher than that in the control group (P 0.05). Compared with the control group, there was no significant difference in 0.62 vs 0.64N 0.79 vs 0.73N 0.49 vs 0.50). Conclusion the prescription can promote the expression of Bcl-2 protein, inhibit the expression of Bax-Cas-pase3 gene and protein, inhibit the apoptosis of ovarian granulosa cells to the level of natural ovulation mice, and improve the follicle quality of superovulation mice.
【作者單位】： 廣州軍區(qū)廣州總醫(yī)院生殖醫(yī)學(xué)中心;廣州中醫(yī)藥大學(xué);汕頭大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院康復(fù)科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(編號(hào):81403249)
【分類號(hào)】：R271.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 郭新宇;張金玉;李海霞;謝妍;袁啟龍;鄧偉民;;中藥益氣血方對(duì)超促排卵小鼠卵巢生長(zhǎng)分化因子表達(dá)的影響[J];廣州中醫(yī)藥大學(xué)學(xué)報(bào);2012年06期

2 金艷梅;;顆粒細(xì)胞對(duì)卵泡發(fā)育的影響[J];中國(guó)畜牧獸醫(yī);2010年08期

3 張金玉;鄧偉民;葛明曉;郭新宇;于妍;謝素嫣;;益氣血補(bǔ)肝法在體外受精—胚胎移植治療周期中對(duì)雌二醇、孕酮水平的影響[J];廣州中醫(yī)藥大學(xué)學(xué)報(bào);2008年06期

4 鄧偉民;邵玉;張金玉;葛明曉;郭新宇;;中藥益氣血補(bǔ)肝腎法對(duì)輔助生育胚胎種植率的影響[J];廣州中醫(yī)藥大學(xué)學(xué)報(bào);2008年02期

5 李培旭;李尚為;彭芝蘭;黃仲英;馬黔紅;李蕾;靳松;;超排卵對(duì)小鼠胚胎著床潛能的影響[J];實(shí)用婦產(chǎn)科雜志;2007年04期

6 張曉紅,楊正望,尤昭玲;補(bǔ)腎中藥對(duì)卵巢功能及子宮內(nèi)膜的影響[J];中醫(yī)藥學(xué)刊;2005年11期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 許露;李燕;;TUNEL法探討以“肝脾”為核心對(duì)高催乳激素血癥模型大鼠卵巢顆粒細(xì)胞凋亡的影響[J];貴陽(yáng)中醫(yī)學(xué)院學(xué)報(bào);2017年04期

2 楊貞;郭新宇;李海霞;劉佳子;鄧偉民;;益氣血法對(duì)超排卵小鼠卵巢顆粒細(xì)胞Bcl-2、Bax、Caspase3表達(dá)的影響[J];實(shí)用醫(yī)學(xué)雜志;2017年13期

3 黃映紅;李宛靜;徐曉娟;鄧蒂斯;雒芙蓉;張純;;補(bǔ)腎化痰方對(duì)卵巢顆粒細(xì)胞脂聯(lián)素依賴p38MAPK途徑及增殖分化相關(guān)信號(hào)分子的調(diào)控作用[J];時(shí)珍國(guó)醫(yī)國(guó)藥;2017年06期

4 楊貞;郭新宇;鄧偉民;張金玉;袁人飛;湯璐璐;;益氣血法對(duì)超排卵大鼠卵巢顆粒細(xì)胞凋亡及Bim表達(dá)的影響[J];實(shí)用醫(yī)學(xué)雜志;2017年10期

5 龐麗娥;吳麗敏;;卵巢儲(chǔ)備功能下降的發(fā)病機(jī)制及補(bǔ)腎活血法治療進(jìn)展[J];山東中醫(yī)雜志;2017年04期

6 徐劍華;李鵬;;Ras-MAPK信號(hào)通路對(duì)蛋雞卵泡顆粒細(xì)胞STMN2蛋白表達(dá)的影響[J];黑龍江八一農(nóng)墾大學(xué)學(xué)報(bào);2017年02期

7 李悅;計(jì)紅;薛琳琳;馬莉;楊煥民;連帥;郭文晉;張宇辰;翟俊飛;郭景茹;劉艷芝;甄莉;;不同培養(yǎng)時(shí)間對(duì)雞卵泡顆粒細(xì)胞孕酮、雌激素分泌水平及FSHR、LHR基因表達(dá)的影響[J];中國(guó)應(yīng)用生理學(xué)雜志;2017年02期

8 張曉麗;趙曉勇;;滋腎育胎丸對(duì)免疫性不孕模型大鼠卵母細(xì)胞生物學(xué)行為的調(diào)節(jié)作用[J];中國(guó)中西醫(yī)結(jié)合雜志;2017年03期

9 屈文;金志春;趙剛;余啟枝;鄭艷華;劉]斊，

本文編號(hào)：2047704