本文選題：電針 + 胃潰瘍　； 參考：《中華中醫(yī)藥雜志》2017年07期

【摘要】：目的:采用核磁共振氫譜技術(shù)(1H NMR)研究電針胃經(jīng)穴治療應(yīng)激性胃潰瘍大鼠大腦皮質(zhì)、下丘腦、延髓代謝物譜的表達(dá)。方法:40只Wistar大鼠隨機分為正常組、模型組、胃經(jīng)組和膽經(jīng)組。利用束縛冷應(yīng)激法制備應(yīng)激性胃潰瘍模型大鼠,造模成功后用電針分別刺激胃經(jīng)組和膽經(jīng)組相關(guān)穴位。收集大鼠大腦皮質(zhì)、下丘腦、延髓,獲取一維1H NMR譜,并利用模式識別方法分析各組間的代謝輪廓差異。結(jié)果:模型組與正常組、胃經(jīng)組、膽經(jīng)組大鼠大腦皮質(zhì)、下丘腦和延髓代謝物譜均有明顯區(qū)分,其中正常組與模型組分離最明顯,胃經(jīng)組最接近正常組,說明其代謝物譜趨向正常。與模型組比較,胃經(jīng)組大鼠大腦皮質(zhì)乳酸、萘乙酸、甘露醇等表達(dá)上升(P0.05),而肌酸、腺苷、肌苷等表達(dá)下降(P0.05),下丘腦乳酸、肌醇、谷氨酸酯等表達(dá)上升(P0.05),γ-氨基丁酸等表達(dá)下降(P0.05),延髓乳酸、谷氨酸脂、肌醇、丙氨酸等表達(dá)上升(P0.05),而萘乙酸、肌酸等表達(dá)下降(P0.05)。結(jié)論:電針胃經(jīng)穴可調(diào)節(jié)胃潰瘍大鼠大腦皮質(zhì)、下丘腦、延髓的代謝產(chǎn)物表達(dá),大腦皮質(zhì)、下丘腦和延髓是電針胃經(jīng)穴治療胃潰瘍的中樞響應(yīng)位點。
[Abstract]:Objective: to study the expression of metabolites in cerebral cortex, hypothalamus and medulla oblongata of rats with stress gastric ulcer treated by electroacupuncture at gastric meridian by 1H NMR. Methods 40 Wistar rats were randomly divided into normal group, model group, gastric meridian group and gallbladder meridian group. The rat model of gastric ulcer induced by stress was prepared by the method of restraint cold stress. After the model was successfully made, the acupoints of gastric meridian group and gallbladder meridian group were stimulated by electroacupuncture respectively. The 1H NMR spectra of cerebral cortex, hypothalamus and medulla oblongata of rats were collected, and the difference of metabolic profile was analyzed by pattern recognition method. Results: the metabolites spectrum of cerebral cortex, hypothalamus and medulla oblongata in model group and normal group, gastric meridian group and gallbladder meridian group were distinguished obviously, among them, the separation between normal group and model group was the most obvious, and the gastric meridian group was closest to normal group. The results showed that the metabolite spectra tended to be normal. Compared with the model group, the expressions of lactic acid, naphthalene acetic acid and mannitol in the gastric meridian group increased, while the expressions of creatine, adenosine and inosine decreased P0.05, lactic acid and inositol in the hypothalamus. The expression of Glutamic acid, 緯 -aminobutyric acid and so on decreased P0.05A, the expressions of lactic acid, glutamate, inositol and alanine increased, while the expressions of naphthyl acetic acid and creatine decreased. Conclusion: electroacupuncture can regulate the expression of metabolites in cerebral cortex, hypothalamus and medulla of rats with gastric ulcer. The central response sites of gastric ulcer treated by electroacupuncture of gastric meridians are cerebral cortex, hypothalamus and medulla oblongata.
【作者單位】： 廈門大學(xué)中醫(yī)系;廈門大學(xué)深圳研究院;廈門大學(xué)電子科學(xué)系福建省等離子體與磁共振研究重點實驗室;
【基金】：國家自然科學(xué)基金項目(No.81473751,No.81260556) 廣東省自然科學(xué)基金項目(No.2014A030313005) 深圳市科技計劃項目(No.JCYJ20160406140612883)~~
【分類號】：R245

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