本文選題：經(jīng)穴反應(yīng)性 + 時(shí)間特征��； 參考：《成都中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的：根據(jù)針刺后不同部位TRPV1、TRPV4受體的改變探討經(jīng)穴反應(yīng)性時(shí)間動(dòng)態(tài)變化的機(jī)理。方法：實(shí)驗(yàn)分為兩部分進(jìn)行：(1)將清潔級(jí)的雄性SD大鼠光暗馴化7天后,先進(jìn)行基礎(chǔ)痛閾的篩選,然后按照痛閾結(jié)果進(jìn)行隨機(jī)分組,將大鼠分為造模組和空白組；造模組在右足足墊部注射完全弗氏佐劑。造模成功后又按基礎(chǔ)痛閾將大鼠隨機(jī)分為模型組和模針組,每組均按照不同的處理時(shí)間點(diǎn)分為ZT0(7:00)、ZT4 (11:00)、ZT8(15:00)、ZT12(19:00)、ZT16(23:00)、ZT20(3:00)6個(gè)亞組。在6個(gè)不同時(shí)間點(diǎn)進(jìn)行處理,模針組在相應(yīng)時(shí)間點(diǎn)手針針刺大鼠患側(cè)足三里30min,其間每5分鐘捻轉(zhuǎn)1min,頻率為120次/min�？瞻捉M和模型組捆綁固定30min,不針刺。治療結(jié)束后運(yùn)用甩尾法檢測不同時(shí)間點(diǎn)大鼠痛閾變化。(2)根據(jù)實(shí)驗(yàn)一重復(fù)測量方差分析的結(jié)果,選取針刺后痛閾曲線最高點(diǎn)和最低點(diǎn)對(duì)應(yīng)的時(shí)相點(diǎn)進(jìn)行實(shí)驗(yàn),分組及造模方法同前,針刺治療7天,在第7天治療結(jié)束后立即取材,采用免疫組化的方法檢測大鼠穴位局部、背根神經(jīng)節(jié)以及脊髓中TRPV1、TRPV4的表達(dá),比較經(jīng)穴反應(yīng)時(shí)間動(dòng)態(tài)性與TRPV1、TRPV4的關(guān)系。結(jié)果：1、時(shí)間因素對(duì)空白組痛閾有顯著影響,F=9.205,P=0.00,P0.05；痛閾的峰值出現(xiàn)在ZTO,谷值分別出現(xiàn)在ZT4和ZT12。2、造模后,大鼠痛閾明顯下降,節(jié)律紊亂,時(shí)間因素對(duì)模型組痛閾無明顯影響,F=1.155,P=0.3540.05。3、針刺后,大鼠痛閾明顯升高,時(shí)間因素對(duì)痛閾的影響有顯著意義,F=30.414,P=0.0000.05。其中針刺后痛閾升高最明顯的時(shí)間點(diǎn)為ZT8,最不明顯的時(shí)間點(diǎn)為ZT16。4、穴位局部皮膚：ZT8時(shí)間點(diǎn),與空白組相比,模型組大鼠皮膚的TRPV1、TRPV4表達(dá)明顯上調(diào)(P0.05或P0.01)；與模型組相比,模針組TRPV1、TRPV4含量顯著下降(P0.05)。ZT16時(shí)間點(diǎn)中：與空白組相比,模型組大鼠皮膚的TRPV1、 TRPV4表達(dá)明顯上調(diào)(P0.05)；針刺后,模針組大鼠皮膚TRPV1的含量與模型組比較,明顯下降(P0.05),但TRPV4勺含量與模型組相比無明顯差異(P0.05)。5、背根神經(jīng)節(jié)：ZT8時(shí)間點(diǎn),模型組大鼠背根神經(jīng)節(jié)TRPV1、TRPV4含量與空白組相比,明顯增加(P0.01),模針組大鼠背根神經(jīng)節(jié)TRPV1、TRPV4含量與模型組相比,含量顯著下降(P0.01或P0.05)。ZT16時(shí)間點(diǎn)：模型組背根神經(jīng)節(jié)TRPV1、TRPV4含量非常明顯增加(P0.01或P0.05)；與模型組相比,模針組背根神經(jīng)節(jié)TRPV1含量明顯下降(P0.05),但TRPV4含量與模型組相比無明顯差異(P0.05)。6、在脊髓：ZT8時(shí)間點(diǎn),模型組的TRPV1、TRPV4含量與空白組相比,明顯增加(P0.01或P0.05)；與模型組相比,模針組的TRPV1含量明顯下降(P0.01),但TRPV4蛋白含量無明顯差異(P0.05)。ZT16時(shí)間點(diǎn),模型組的TRPV1、TRPV4含量明顯增加(P0.01)；針剌后,模針組TRPV1、TRPV4含量與模型組相比明顯減少(P0.05)。結(jié)論：1、正常大鼠痛閾呈現(xiàn)節(jié)律性波動(dòng),受時(shí)間因素的影響。表現(xiàn)為雙谷結(jié)構(gòu),谷值分別出現(xiàn)在ZT4和ZT12。2、經(jīng)穴反應(yīng)具有時(shí)間動(dòng)態(tài)性變化,不同時(shí)間點(diǎn)針刺均可提高大鼠的痛閾,針刺后穴位反應(yīng)性的強(qiáng)弱受時(shí)間因素的影響,表現(xiàn)為單峰一谷的結(jié)構(gòu)。不同時(shí)間針刺對(duì)痛閾的影響不同,說明穴位的反應(yīng)性不同,其中針刺后經(jīng)穴反應(yīng)最佳時(shí)間點(diǎn)為ZT8、最差的時(shí)間點(diǎn)為ZT16。3、經(jīng)穴反應(yīng)性時(shí)間動(dòng)態(tài)變化可能與針刺后穴位局部、背根神經(jīng)節(jié)、脊髓TRPV1、 TRPV4興奮的數(shù)量以及種類有關(guān)。
[Abstract]:Objective: To explore the mechanism of dynamic changes in the response time of the meridians according to the changes of TRPV1 and TRPV4 receptors in different parts of the post acupuncture. Methods: the experiment was divided into two parts: (1) after the dark acclimation of the clean male SD rats for 7 days, the basic pain threshold was screened first, and then the rats were randomly divided into the models according to the pain threshold, and the rats were divided into models. Group and blank group; the model group was injected with complete Freund's adjuvant at the right foot pad. After the success of the model, the rats were randomly divided into the model group and the model needle group according to the basic pain threshold. Each group was divided into ZT0 (7:00), ZT4 (11:00), ZT8, ZT12, ZT16, ZT20, at different time points. At the corresponding time point, the group was treated with the hand acupuncture at the corresponding time point of the rats with the 30min of the Zusanli, which was twirling 1min every 5 minutes. The frequency of the 120 times /min. blank group and the model group were bound to the fixed 30min, without acupuncture. After the treatment, the pain threshold of the rats at different time points was detected by the tail flick method. (2) according to the result of the analysis of variance analysis of one repeated test, the results were selected. After the acupuncture, the highest point of pain threshold curve and the time point corresponding to the lowest point were tested. The group and the modeling method were the same before the acupuncture treatment for 7 days. After the treatment at the end of the seventh day, the material was taken immediately. The immunohistochemical method was used to detect the expression of TRPV1 and TRPV4 in the dorsal root ganglion and spinal cord, and the time dynamic of the acupoint reaction and the TRPV were compared. 1, TRPV4. Results: 1, time factors have a significant effect on the pain threshold of blank group, F=9.205, P=0.00, P0.05; the peak of pain threshold appears in ZTO, the valley value appears in ZT4 and ZT12.2 respectively. After the model, the pain threshold of the rat is obviously decreased and the rhythm is disordered. The time factors have no obvious effect on the pain threshold of the model group, F=1.155, P=0.3540.05.3, and acupuncture, pain threshold of rats. The effect of time factors on pain threshold was significant, F=30.414, P=0.0000.05., the most obvious time point was ZT8 after acupuncture, the most obvious time point was ZT16.4, the local skin of acupoint: ZT8 time point, TRPV1 of skin skin in model group, TRPV4 expression was obviously up up (P0.05 or P0.01), and the model was compared with the blank group. Compared with the model group, the content of TRPV1 and TRPV4 decreased significantly (P0.05).ZT16 time point: compared with the blank group, the expression of TRPV1 and TRPV4 in the model group was obviously up (P0.05). After acupuncture, the content of TRPV1 in the skin of the rat model group was significantly lower than that in the model group (P0.05), but there was no significant difference between the TRPV4 spoon content and the model group (P0.). 05).5, dorsal root ganglion: ZT8 time point, model group rat dorsal root ganglion TRPV1, TRPV4 content compared with the blank group, significantly increased (P0.01), the rat dorsal root ganglion TRPV1, TRPV4 content compared with the model group, the content was significantly decreased (P0.01 or P0.05).ZT16 time point: the model group dorsal root ganglion TRPV1, TRPV4 content was remarkably increased ( P0.01 or P0.05); compared with the model group, the TRPV1 content in the dorsal root ganglion of the model group was significantly decreased (P0.05), but the TRPV4 content was not significantly different from the model group (P0.05).6. In the spinal cord: ZT8 time point, the content of TRPV1 in the model group was significantly higher than that in the blank group (P0.01 or P0.05). Compared with the model group, the content of the model group was obvious. Decrease (P0.01), but there was no significant difference in TRPV4 protein content (P0.05).ZT16 time point, TRPV1 and TRPV4 content in model group increased significantly (P0.01); after acupuncture, the content of TRPV4 in model needle group was significantly reduced (P0.05) compared with model group (P0.05). Conclusion: 1, normal rats' pain threshold showed rhythmic fluctuation, influenced by time factors. The expression was double valley structure, valley The value of the acupoint reaction has time dynamic change, and the acupuncture at different time points can improve the pain threshold of the rats. The effect of acupuncture at different time points can increase the pain threshold of the rat. The intensity of acupoint reactivity after acupuncture is influenced by the time factor, which is the structure of single peak and one valley. The effect of acupuncture on pain threshold at different time is different, which shows that the reactivity of acupoints is different, among which acupuncture after acupuncture is different. After acupuncture, the effect of acupuncture on the pain threshold is different, among which acupuncture after acupuncture is different. The best time point of acupoint reaction is ZT8 and the worst time point is ZT16.3. The dynamic changes of the acupoint reactivity time may be related to the number and type of the acupoint, the dorsal root ganglion, the spinal cord TRPV1 and the TRPV4, after the acupuncture.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R246

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃麗潔,解立新,賈琳,趙曉巍,劉又寧,茍鵬;雜種犬急性呼吸窘迫綜合征模型的建立[J];中國比較醫(yī)學(xué)雜志;2004年04期

2 張臣顥;張曼芳;謝青蓮;成志恒;鄒洪;金玫蕾;;慢性不可預(yù)知溫和應(yīng)激抑郁小鼠模型的建立[J];復(fù)旦學(xué)報(bào)(自然科學(xué)版);2011年03期

3 譚瀟;肖建華;;1型糖尿病小鼠模型的構(gòu)建[J];國際檢驗(yàn)醫(yī)學(xué)雜志;2011年14期

4 任文英,陳香美,邱全瑛,陳揚(yáng)榮,王新高,師鎖柱,王兆霞;慢性移植物抗宿主病狼瘡樣小鼠模型的誘導(dǎo)[J];中國比較醫(yī)學(xué)雜志;2004年04期

5 王艷春,呂莉;彌散性血管內(nèi)凝血模型的改進(jìn)[J];第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院學(xué)報(bào);2005年02期

6 陳小囡;;蜂膠對(duì)慢性酒精性肝損傷的保護(hù)作用及其機(jī)制研究[J];健康研究;2009年02期

7 武君;孫長崗;鄭華;;愈寧散對(duì)CITP模型小鼠外周血共刺激分子CD86影響的實(shí)驗(yàn)研究[J];實(shí)用中醫(yī)內(nèi)科雜志;2010年09期

8 王紫;陳瑜;羅軍;周建華;;槐杞黃干預(yù)小鼠IgA腎病模型的療效觀察以及機(jī)制探討[J];中國中西醫(yī)結(jié)合腎病雜志;2011年11期

9 孫旖;夏振娜;袁伯俊;張曉芳;陸國才;;LT-Y008的骨髓保護(hù)作用及機(jī)制[J];中國藥理學(xué)與毒理學(xué)雜志;2013年03期

10 周厚廣,鮑遠(yuǎn)程,陸建明;提高帕金森病動(dòng)物模型制作成功率的方法學(xué)探討及模型綜合評(píng)價(jià)[J];國際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2005年04期

相關(guān)會(huì)議論文 前10條

1 閻明;張喜紅;劉莉;孟繁立;張莉;朱萍;;美能對(duì)酒精性肝纖維化大鼠的干預(yù)及治療研究[A];第五屆全國肝臟疾病臨床暨中華肝臟病雜志成立十周年學(xué)術(shù)會(huì)議論文匯編[C];2006年

2 王勇炫;吳偉康;吳以嶺;張選紅;;蜈蚣有效部位抗心肌缺血的NO機(jī)制研究[A];2009年全國中藥學(xué)術(shù)研討會(huì)論文集[C];2009年

3 周友龍;李建生;高劍峰;;老齡大鼠腦缺血再灌注微血管再生的變化意義[A];中國中西醫(yī)結(jié)合學(xué)會(huì)第五屆基礎(chǔ)理論研究專業(yè)委員會(huì)學(xué)術(shù)研討會(huì)論文集[C];2006年

4 趙萬軍;周輝;韓勇;何永江;;丹參對(duì)激素性股骨頭壞死血管內(nèi)凝血的影響[A];2007年浙江省醫(yī)學(xué)會(huì)骨科學(xué)學(xué)術(shù)會(huì)議暨浙江省抗癌協(xié)會(huì)骨軟腫瘤學(xué)術(shù)會(huì)議論文匯編[C];2007年

5 孫元瑩;史曉峰;;益肺康顆粒對(duì)油酸致兔急性肺損傷實(shí)驗(yàn)研究[A];2003年全國危重病急救醫(yī)學(xué)學(xué)術(shù)會(huì)議論文集[C];2003年

6 孫元瑩;史曉峰;;益肺康顆粒對(duì)油酸致兔急性肺損傷實(shí)驗(yàn)研究[A];2004年全國危重病急救醫(yī)學(xué)學(xué)術(shù)會(huì)議論文集[C];2004年

7 李玉中;;復(fù)方甘正對(duì)非酒精性脂肪性肝炎的治療作用[A];第五次全國中青年檢驗(yàn)醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2006年

8 孫旖;夏振娜;袁伯俊;張曉芳;陸國才;;LT-Y008的骨髓保護(hù)作用及機(jī)制[A];2013年（第三屆）中國藥物毒理學(xué)年會(huì)暨藥物非臨床安全性評(píng)價(jià)研究論壇論文摘要[C];2013年

9 孫旖;夏振娜;袁伯俊;張曉芳;陸國才;;LT-Y008的骨髓保護(hù)作用及機(jī)制[A];中國藥理學(xué)與毒理學(xué)雜志(2013年6月第27卷第3期)[C];2013年

10 羅道樞;王瑋;;細(xì)胞外信號(hào)調(diào)節(jié)激酶在三叉神經(jīng)痛動(dòng)物模型中的活化[A];2007年中國解剖學(xué)會(huì)第十屆全國組織學(xué)與胚胎學(xué)青年學(xué)術(shù)研討會(huì)論文摘要匯編[C];2007年

相關(guān)重要報(bào)紙文章 前1條

1 武漢市中西醫(yī)結(jié)合醫(yī)院 單萍;黃角顆�？鼓X梗死作用機(jī)理[N];中國中醫(yī)藥報(bào);2007年

相關(guān)博士學(xué)位論文 前10條

1 烏日?qǐng)D那順;蒙藥“伊赫湯”對(duì)酒精性脂肪肝作用機(jī)理的實(shí)驗(yàn)研究[D];北京中醫(yī)藥大學(xué);2015年

2 沈斌;肝豆靈對(duì)銅負(fù)荷大鼠肝組織的miRNA-122和代謝組學(xué)的影響[D];湖北中醫(yī)藥大學(xué);2015年

3 趙賓賓;地五養(yǎng)肝膠囊抑制2-AAF/PH大鼠肝癌前病變的作用及機(jī)制[D];湖北中醫(yī)藥大學(xué);2015年

4 張海嬰;外敷清肺通絡(luò)膏干預(yù)RSV大鼠肺炎TLR4信號(hào)通路機(jī)制研究[D];遼寧中醫(yī)藥大學(xué);2015年

5 侯燕;冠狀動(dòng)脈粥樣硬化模型豬膽固醇代謝異常對(duì)Aβ的影響及復(fù)方干預(yù)研究[D];遼寧中醫(yī)藥大學(xué);2015年

6 王榮田;股骨頭壞死的證素組合規(guī)律與中藥干預(yù)的實(shí)驗(yàn)研究[D];湖南中醫(yī)藥大學(xué);2009年

7 李思耐;基于L型鈣電流研究益氣藥干預(yù)心衰電生理重構(gòu)機(jī)制[D];北京中醫(yī)藥大學(xué);2016年

8 高允海;愈膽湯防治膽囊切除術(shù)后綜合征的臨床與實(shí)驗(yàn)研究[D];遼寧中醫(yī)藥大學(xué);2016年

9 胡文軍;復(fù)方腦康膠囊治療Alzheimer's病的藥效學(xué)研究[D];南方醫(yī)科大學(xué);2012年

10 王濤;普利醇抗帕金森病的藥效學(xué)及其機(jī)制研究[D];中國協(xié)和醫(yī)科大學(xué);2009年

相關(guān)碩士學(xué)位論文 前10條

1 吳謀東;黃芪、冬蟲夏草對(duì)新生鼠腎積水梗阻解除后腎損害恢復(fù)的影響[D];遵義醫(yī)學(xué)院;2015年

2 張子洋;醒腦液對(duì)APPswe/PS1ΔE9雙轉(zhuǎn)基因小鼠腦內(nèi)Aβ及其降解酶NEP表達(dá)的影響[D];河北聯(lián)合大學(xué);2014年

3 韓金麗;急性胰腺炎大鼠神經(jīng)降壓素水平與腸黏膜損傷關(guān)系的實(shí)驗(yàn)研究[D];河北醫(yī)科大學(xué);2015年

4 潘彩彬;膝關(guān)節(jié)炎治療儀對(duì)膝骨性關(guān)節(jié)炎軟骨形態(tài)及關(guān)節(jié)液細(xì)胞因子影響的實(shí)驗(yàn)研究[D];福建中醫(yī)藥大學(xué);2015年

5 浦飛飛;敬釗纓毛蜘蛛毒素-V對(duì)LTP和認(rèn)知行為改善作用的分子機(jī)制研究[D];北京協(xié)和醫(yī)學(xué)院;2015年

6 崔慶飛;酒精聯(lián)合高脂飲食誘導(dǎo)小鼠脂肪肝模型的建立及發(fā)病機(jī)制探討[D];河北醫(yī)科大學(xué);2015年

7 劉翠華;谷氨酰胺對(duì)膿毒癥小鼠氧化應(yīng)激作用效果的研究[D];河北醫(yī)科大學(xué);2015年

8 岳萌;Bufalin通過ERK/RSK2通路對(duì)ECA109細(xì)胞和裸鼠移植瘤作用的研究[D];河北醫(yī)科大學(xué);2015年

9 左玲;Xyloketal B對(duì)NAFLD的療效觀察和機(jī)制研究[D];暨南大學(xué);2015年

10 郎艷松;通心絡(luò)聯(lián)合阿托伐他汀、阿司匹林對(duì)新西蘭兔動(dòng)脈粥樣硬化早期頸動(dòng)脈外膜滋養(yǎng)血管新生相關(guān)炎癥機(jī)制的影響[D];河北醫(yī)科大學(xué);2015年

，

本文編號(hào)：2013645