本文選題：推拿 + 參數(shù)��； 參考：《北京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：[目的]通過正交試驗指導(dǎo)下比較行為學(xué)、腓腸肌功能、免疫組織化學(xué)相關(guān)指標(biāo)的改變,從行為學(xué)下研究推拿手法參數(shù)中的力量、時間、頻率對周圍神經(jīng)損傷大鼠運動功能恢復(fù)的影響。探究推拿促進坐骨神經(jīng)損傷大鼠運動功能恢復(fù)的最佳刺激參數(shù)組合。[方法]以坐骨神經(jīng)損傷為例研究周圍神經(jīng)損傷,以SD大鼠為實驗動物,采用坐骨神經(jīng)加持損傷模型。7天后使用"推拿手法模擬儀"模擬三法(撥法、揉法、點法)推拿手法在三個穴道(殷門、承山、陽陵泉)上進行干預(yù)。以力量(A)、時間(B)、頻率(C)三種因素,每個因數(shù)各有三組不同水平刺激,力量為2N、4N、6N;時間1min、3min、5min;頻率30次/min、60次/min、100次/min。用三因素三水平的正交試驗設(shè)計方案篩選推拿干預(yù)促進SNI大鼠運動功能恢復(fù)的最佳刺激參數(shù)組合。1.從行為學(xué)角度,測定斜板實驗及坐骨神經(jīng)功能指數(shù)以評價大鼠肌力功能恢復(fù)情況。2.從功能角度,測定腓腸肌濕重恢復(fù)率評價推拿對肌肉萎縮程度的改善。3.采用免疫組織化學(xué)法測定脊髓腹角內(nèi)NGF的表達情況,探尋推拿促進NGF表達的最佳刺激參數(shù)。[結(jié)果]1.斜板實驗造模7天后斜板實驗數(shù)據(jù)分析,正常組、假手術(shù)組、模型組之間存在統(tǒng)計學(xué)差異(P0.01)。推拿干預(yù)20次后,推拿組(九組)之間采用正交試驗的直觀分析法,研究結(jié)果顯示三因素的主次關(guān)系為:時間力量頻率。最佳刺激參數(shù)組合是力量4N,時間1min,頻率60次/min。推拿組(九組)之間采用方差分析法得出,三因素力量(A)、時間(B)、頻率(C)對結(jié)果的影響無統(tǒng)計學(xué)差異(P0.05)。但從F值的大小判斷(正交試驗中對應(yīng)因素的F值越大,該因素對結(jié)果的影響也越大),來看,可認(rèn)為各因素的主次關(guān)系為:時間力量頻率,而以短時間治療效果為佳。這與直觀分析法得出的結(jié)果一致。2.坐骨神經(jīng)功能指數(shù)推拿干預(yù)20次后,推拿組(九組)之間采用正交試驗的直觀分析法,研究結(jié)果顯示三因素的主次關(guān)系為:頻率時間力量。最佳刺激參數(shù)組合是力量2N,時間1min,頻率30次/mmin。推拿組(九組)之間采用方差分析法得出,三因素力量(A)、時間(B)、頻率(C)對結(jié)果的影響無統(tǒng)計學(xué)差異(P0.05)。但從F值的大小判斷(對應(yīng)因素的F值越大,該因素對結(jié)果的影響也越大),來看,可認(rèn)為各因素的主次關(guān)系為:頻率時間力量,這與直觀分析法得出的結(jié)果一致。3.腓腸肌濕重恢復(fù)率造模7天后,推拿組與模型組腓腸肌濕重恢復(fù)率無顯著性差異(P0.05),推拿干預(yù)20次后,推拿組腓腸肌濕重恢復(fù)率測定結(jié)果與模型組比較有顯著性差異(P0.05),代表腓腸肌萎縮后進行推拿干預(yù)可有預(yù)防肌肉萎縮的效果。推拿干預(yù)20次后,推拿組(九組)之間采用正交試驗的直觀分析法,研究結(jié)果顯示三因素的主次關(guān)系為力量＞頻率時間,根據(jù)F值的大小,最佳刺激參數(shù)組合是力量4N,時間5mmin,頻率30次/min。推拿組(九組)之間采用方差分析法得出,三因素力量(A)、時間(B)、頻率(C)的主次關(guān)系無顯著性差異(P0.05)。但從F值的大小判斷(對應(yīng)因素的F值越大,該因素對結(jié)果的影響也越大),來看,可認(rèn)為各因素的主次關(guān)系為:力量頻率時間,這與直觀分析法得出的結(jié)果一致。4.脊髓腹角NGF光密度推拿干預(yù)20次后,推拿組(九組)之間采用正交試驗的直觀分析法,研究結(jié)果顯示三因素的主次關(guān)系為:力量時間頻率。最佳刺激參數(shù)組合是力量4N,時間1min,頻率60次/min。推拿組(九組)之間采用方差分析法得出,三因素力量(A)、時間(B)、頻率(C)對結(jié)果的影響有統(tǒng)計學(xué)差異(P0.05)。各因素的主次關(guān)系為力量時間頻率,但從單因素來看,A(力量)因素、B(時間)因素的影響顯著(P0.05),C(頻率)因素的影響不顯著(P0.05)。[結(jié)論]1.通過三法三穴干預(yù)可以促進SNI大鼠運動功能的恢復(fù),刺激參數(shù)為中等力量、短時間、中等頻率為佳。而手法力量過重、時間過長時療效反而降低。2.通過三法三穴干預(yù)可以調(diào)控SNI大鼠脊髓腹角NGF的表達。通過NGF的提高可使運動功能恢復(fù)而刺激參數(shù)以中等力量、短時間、中頻率為佳。
[Abstract]:[Objective] to compare the effect of behavior, gastrocnemius function and immuno histochemical related indexes under the guidance of orthogonal test, and study the influence of the strength, time and frequency of the manipulative parameters on the recovery of motor function in rats with peripheral nerve injury. With the sciatic nerve injury as an example, the peripheral nerve injury was studied with the sciatic nerve injury as an example. SD rats were used as experimental animals. The three methods (dial method, kneading method, point method) were used to intervene in the three Cave (Yin gate, Chengshan and Yang Ling spring) using the sciatic nerve injury model after.7 days. ), three factors of frequency (C), each factor has three groups of different levels of stimulation, power is 2N, 4N, 6N, time 1min, 3min, 5min, frequency 30 /min, 60 /min, 100 /min. with three factors three level orthogonal design scheme screening the best stimulus parameter combination to promote the function recovery of SNI rats. The oblique plate experiment and the sciatic nerve function index to evaluate the recovery of muscle function in rats.2. from the functional point of view, the evaluation of the recovery rate of the gastrocnemius wet weight and the improvement of the degree of muscular atrophy by the massage.3., the expression of NGF in the ventral horn of the spinal cord was measured by immunohistochemistry, and the best stimulation parameters to promote the expression of NGF were explored. [result]1. There was a statistical difference between the normal group, the sham operation group and the model group (P0.01). After 20 times of the massage intervention, the orthogonal test was used by the massage group (nine groups). The results showed that the main and secondary relation of the three factors was the time force frequency. The best combination of the stimulus parameters was the strength 4N, Time 1min, frequency 60 times /min. massage group (nine groups) between the use of variance analysis method, three factor power (A), time (B), frequency (C) on the effect of no statistical difference (P0.05). But from the size of the F value (the greater the F value of the corresponding factors in the orthogonal test, the greater the impact of the factor on the results), it is considered that the main and secondary factors of the factors can be considered. The relationship is: time power frequency, and the effect of short time treatment is better. This is consistent with the results obtained by the intuitionistic analysis method. After 20 times of.2. sciatic nerve function index massage intervention, the intuitionistic analysis of the orthogonal test between the massage group (nine groups) is adopted, and the results show that the main and secondary relations of the three factors are the frequency time force and the best stimulus parameter group. The combination is power 2N, time 1min, frequency 30 times /mmin. massage group (nine groups) using variance analysis method, three factor power (A), time (B), frequency (C) has no statistical difference (P0.05). But judging from the size of F value (the greater the F value of the corresponding factor, the greater the effect of the factor on the result), it can be considered the main factor. The secondary relationship was: frequency time force, which was consistent with the results obtained by the visual analysis method, the recovery rate of gastrocnemius wet weight recovery rate of.3. was no significant difference (P0.05) after 7 days. After 20 times of massage, the determination of the recovery rate of the gastrocnemius wet weight in the massage group was significantly different from the model group (P0.05), and the generation of the gastrocnemius muscle weight recovery rate was significantly different from the model group. The effect of massage after gastrocnemius atrophy could prevent muscle atrophy. After 20 times of massage, a visual analysis of orthogonal test was used between the nine groups of massage group (Group). The results showed that the main and secondary relationship of the three factors was power > frequency time. According to the size of F, the best combination of stimulus parameters was power 4N, time 5mmin, frequency 30. The second /min. massage group (nine groups) by variance analysis method, three factor power (A), time (B), frequency (C) of the primary and secondary relations of no significant difference (P0.05). But from the size of the F value (the value of the corresponding factor, the greater the value of the factor, the greater the impact of the factor on the results), it is considered that the main and secondary relations of the factors are: power frequency time, this is the same. The results of the intuitionistic analysis were consistent with the.4. NGF light density massage for 20 times, and the orthogonal test was used for the intuitional analysis between the massage group (nine groups). The results showed that the main and secondary relations of the three factors were power time frequency. The best combination of stimulus parameters was power 4N, time 1min, and frequency 60 /min. massage groups (nine groups). The results of variance analysis showed that three factors (A), time (B) and frequency (C) had statistical differences (P0.05). The main and secondary relationships of all factors were power time frequency, but from the single factor, the influence of A (strength), B (time) factors was obvious (P0.05) and C (frequency) factors were not significant (P0.05). [conclusion]1. through three methods and three points. Intervention can promote the recovery of motor function of SNI rats. The stimulation parameters are medium strength, short time and moderate frequency. While the manipulative force is too heavy and the time is too long, the curative effect reduces the expression of NGF in the ventral horn of the spinal cord of SNI rats through the intervention of three methods and three points. Through the improvement of NGF, the exercise function can be recovered and stimulate parameters in the middle of the rat. Power, short time, and good frequency.
【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245

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相關(guān)期刊論文 前10條

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本文編號：2009117