本文選題：膠原誘導(dǎo)性關(guān)節(jié)炎 + 類(lèi)風(fēng)濕關(guān)節(jié)炎��； 參考：《桂林醫(yī)學(xué)院》2016年碩士論文

【摘要】：目的:探討三葉青藤木脂素苷(LGIR)類(lèi)成分對(duì)小鼠膠原性關(guān)節(jié)炎的治療作用及對(duì)Th17細(xì)胞的增殖調(diào)控作用及其機(jī)制。方法:用DBA/1小鼠建立CIA小鼠模型,將造模成功的DBA/1小鼠平均分為CIA模型組、甲氨蝶呤(MTX)組、LGIR低、中、高劑量組,分別行藥物灌胃干預(yù),另設(shè)正常對(duì)照組以生理鹽水干預(yù)。干預(yù)結(jié)束后檢測(cè)各組小鼠血清IL-23和IL-17水平,觀察各組小鼠踝關(guān)節(jié)滑膜病理改變,取脾臟并提取淋巴細(xì)胞,應(yīng)用流式細(xì)胞術(shù)(Flow Cytometry,FCM)檢測(cè)Th17細(xì)胞在不同組CIA小鼠淋巴細(xì)胞中的表達(dá)。結(jié)果:1.成功復(fù)制CIA小鼠模型。2.與模型組相比較,LGIR可改善CIA小鼠的AI評(píng)分。3.與模型組相比,LGIR可改善CIA小鼠踝關(guān)節(jié)病理評(píng)分。4.與模型組相比,LGIR可下調(diào)血清IL-23和IL-17水平。5.Th17淋巴細(xì)胞亞群比率的改變:模型組與正常對(duì)照組比較表現(xiàn)為T(mén)h17細(xì)胞亞群比例增加,LGIR高劑量組、MTX組與CIA模型組比較Th17細(xì)胞比例明顯降低。結(jié)論:LGIR能夠改善CIA小鼠局部關(guān)節(jié)腫脹程度,下調(diào)血清IL-23和IL-17水平,減輕關(guān)節(jié)病理改變程度,LGIR高劑量組可以下調(diào)CIA小鼠淋巴細(xì)胞中Th17細(xì)胞亞群比例。
[Abstract]:Aim: to investigate the therapeutic effect of three Ye Qing lividin glycosides on collagen-induced arthritis in mice and its mechanism in regulating the proliferation of Th17 cells. Methods: CIA mouse model was established with DBA / 1 mice. The successful DBA / 1 mice were divided into CIA model group, methotrexate MTX group and low, middle and high dose groups respectively. After the intervention, the levels of IL-23 and IL-17 in serum of each group were measured, the pathological changes of synovial membrane of ankle joint of each group were observed, the spleen was taken and lymphocytes were extracted, and the expression of Th17 cells in the lymphocytes of different groups of CIA mice was detected by flow Cytometry FCM (FCM). The result is 1: 1. The CIA mouse model was successfully established. Compared with model group, LGIR could improve AI score of CIA mice. Compared with the model group, LGIR could improve the ankle pathological score of CIA mice. Compared with the model group, LGIR could down-regulate the changes of serum IL-23 and IL-17 levels. 5. The ratio of Th17 lymphocyte subsets in the model group was significantly lower than that in the normal control group. The ratio of Th17 cell subsets in the model group and the high dose group was significantly lower than that in the CIA model group. Conclusion the weight LGIR can improve the degree of joint swelling in CIA mice, decrease the levels of IL-23 and IL-17 in serum and reduce the degree of pathological changes of joints. LGIR high dose group can down-regulate the proportion of Th17 cell subsets in lymphocytes of CIA mice.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R259

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