本文選題：旱田草 + 質(zhì)量標(biāo)準(zhǔn)��； 參考：《廣西中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:依據(jù)壯藥旱田草來源、性狀、顯微、常規(guī)檢查、浸出物、含量測定和指紋圖譜等系統(tǒng)的研究,初步建立旱田草質(zhì)量標(biāo)準(zhǔn)。方法:通過基原鑒定、性狀鑒定、顯微鑒定與理化鑒定方法系統(tǒng)地對旱田草進(jìn)行生藥學(xué)定性研究;按《中華人民共和國藥典》2015年版四部中規(guī)定的方法對旱田草藥材進(jìn)行限量檢查,包括水分、總灰分、酸不溶性灰分、浸出物等;按照中藥化學(xué)預(yù)實(shí)驗(yàn)方法對旱田草進(jìn)行成分預(yù)試;以毛蕊花糖苷為對照品,采用高效液相色譜法對廣西采集的各批次旱田草藥材進(jìn)行含量測定,確定了其中所含毛蕊花糖苷的限量;并標(biāo)定毛蕊花糖苷為參照峰,運(yùn)用《中藥色譜指紋圖譜相似度評價(jià)系統(tǒng)2012版》軟件對旱田草進(jìn)行初步指紋圖譜研究。結(jié)果:1.原植物鑒定、性狀鑒定、顯微鑒定與薄層色譜鑒定均具顯著性特征,可作為鑒別旱田草藥材真?zhèn)蔚囊罁?jù)。2.旱田草藥材水分含量范圍為8.24%~11.76%,均在12.0%以下,故初步擬定其水分不多于13.0%;總灰分含量范圍為11.62%~17.36%,大多數(shù)在16.0%以下,初步擬定總灰分不超過18.0%;酸不溶性灰分含量范圍為1.55%~8.28%,大部分在7.0%以下,初步擬定酸不溶性灰分不得過9.0%;浸出物含量在17.27%~27.02%之間,均在17.0%以上,初步擬定醇溶性浸出物含量不得少于15.0%。3.預(yù)實(shí)驗(yàn)表明旱田草中含有酚類化合物、三萜化合物、甾體、內(nèi)酯、多糖、氨基酸及揮發(fā)油等成分。4.旱田草最佳提取工藝確定為:取樣量0.4 g,加入70%甲醇20 ml,超聲處理2次,每次超聲30分鐘;毛蕊花糖苷進(jìn)樣量在0.0192~3.84μg之間與峰面積呈良好線性關(guān)系(r=0.99999);平均加樣回收率為103.59%,RSD為2.88%;各批次旱田草藥材中毛蕊花糖苷所測含量在0.23%~0.94%之間,以其中大多數(shù)在0.20%以上為依據(jù),初步擬定其所含毛蕊花糖苷(C16H18O9)不得少于0.20%(2.00mg·g-1)。5.初步建立了旱田草HPLC指紋圖譜提取方法及色譜條件,篩選出15個(gè)共有特征峰,其中以毛蕊花糖苷為參照峰,方法學(xué)考察達(dá)標(biāo),所采集的不同批次旱田草藥材色譜圖匹配度均大于0.96。結(jié)論:以上評價(jià)方法可為壯藥旱田草藥材質(zhì)量標(biāo)準(zhǔn)的建立提供依據(jù),可為其臨床應(yīng)用的安全、有效和質(zhì)量控制奠定基礎(chǔ)。
[Abstract]:Objective: to establish the quality standard of Rhizoma Artemisia on the basis of its origin, characters, microscopes, routine examination, extract, content determination and fingerprint. Methods: the pharmacognosy qualitative studies were carried out systematically by the methods of primordial identification, character identification, microscopical identification and physicochemical identification. According to the methods stipulated in the four parts of the 2015 edition of the Pharmacopoeia of the people's Republic of China, a limited amount of inspection was carried out on the medicinal materials of Artemisia sinensis, including moisture, total ash, acid insoluble ash, extracts, etc. In accordance with the method of chemical pre-experiment of traditional Chinese medicine, the composition of Rhizoma Rutheroside was pre-tested, and the content of each batch of Rhizoma Artemisia collected in Guangxi was determined by HPLC with Rulein as the reference substance. In this paper, the limit of the glucoside contained in Rulein was determined, and the reference peak was calibrated, and the preliminary fingerprint was studied by the software of similarity Evaluation system of chromatographic fingerprint of traditional Chinese Medicine (TCM) version 2012. The result is 1: 1. The original plant identification, character identification, microscopical identification and TLC identification all have significant characteristics, which can be used as the basis for the identification of the true and false medicinal materials. 2. The range of water content of Rhizoma Artemisia is 8.24% and 11.76%, all of which are below 12.0%, so it is preliminarily determined that the water content is not more than 13.0; the total ash content ranges from 11.62% to 17.36%, most of which are below 16.0%. The content of acid insoluble ash was 1.55% or 8.28%, most of which was less than 7.0%, and the content of acid insoluble ash should not exceed 9.0%, and the content of extract was between 17.27% and 27.02%, all of which were above 17.0%, and the total ash content was not more than 18.0%, and the total ash content was less than 18.0%, and the total ash content was 1.55% or 8.28%, most of which was below 7.0%. The content of alcohol-soluble extracts shall not be less than 15.0%. The results showed that there were phenolic compounds, triterpenoids, steroids, lactones, polysaccharides, amino acids and volatile oils. The optimum extraction technology was determined as follows: sampling quantity 0.4 g, adding 70% methanol 20 ml, ultrasonic treatment 2 times, each time ultrasonic 30 minutes; There was a good linear relationship between the sample quantity and peak area between 0.01922 渭 g and 3.84 渭 g, the average recovery rate was 103.59%, RSD was 2.88, and the content of mullein in each batch was between 0.23% and 0.94%, based on the fact that most of them were above 0.20%. It was preliminarily formulated that the content of C16H18O9 was not less than that of 0.20%(2.00mg g-1O. 5. the content of C16H18O9 was not less than that of C16H18O9. The HPLC fingerprint extraction method and chromatographic conditions were established, and 15 common characteristic peaks were screened out, in which Rulein was used as the reference peak, and the methodological investigation was up to standard. The matching degree of the chromatogram of different batches of Herba Artemisia was more than 0.96. Conclusion: the above evaluation methods can provide the basis for the establishment of the quality standard of Herba Artemisia, and lay the foundation for its clinical application safety, effectiveness and quality control.
【學(xué)位授予單位】：廣西中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29

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