本文選題：動(dòng)脈粥樣硬化 + 電針��； 參考：《遼寧中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:觀察電針“足三里”、“內(nèi)關(guān)”、“關(guān)元”穴對動(dòng)脈粥樣硬化模型兔血脂變化的情況,腹腔巨噬細(xì)胞中SR-AI蛋白和m RNA表達(dá)情況的影響,為針刺抗動(dòng)脈粥樣硬化的作用機(jī)制提供新的實(shí)驗(yàn)依據(jù)。材料與方法:將26只健康新西蘭兔適應(yīng)性喂養(yǎng)1周后,隨機(jī)選取7只為空白組喂養(yǎng)標(biāo)準(zhǔn)兔飼料,其余19只為造模組喂養(yǎng)高脂飼料。高脂喂養(yǎng)4周后對造模組行頸總動(dòng)脈球囊損傷術(shù),繼續(xù)高脂喂養(yǎng)4周建立AS模型,從空白組和造模組中各隨機(jī)選取1只實(shí)驗(yàn)兔做頸總動(dòng)脈病理切片,通過HE染色觀察病理形態(tài)確定造模是否成功。隨后將造模組剩余18只實(shí)驗(yàn)兔隨機(jī)分為模型組、西藥組、電針組,每組6只,開始治療。電針組選取“內(nèi)關(guān)”、“足三里”、“關(guān)元”穴電針治療;西藥組給予阿托伐他汀鈣片治療。治療4周后取材,觀察頸總動(dòng)脈病理形態(tài)變化,檢測膽固醇(CHO)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)含量,采用RT-PCR技術(shù)檢測AS實(shí)驗(yàn)兔腹腔巨噬細(xì)胞中SR-AIm RNA的表達(dá)情況,采用Western-blot法檢測SR-AI蛋白的表達(dá)情況。結(jié)果:1與空白組比較,模型組、電針組、西藥組實(shí)驗(yàn)兔CHO、TG、LDL含量均升高,而HDL含量降低,差異有統(tǒng)計(jì)學(xué)意義(P0.01);與模型組比較,電針組、西藥組實(shí)驗(yàn)兔CHO、TG、LDL含量均降低,而HDL含量增高,差異有統(tǒng)計(jì)學(xué)意義(P0.01);電針組與西藥組組間無顯著差異(P0.05)。2與空白組比較,模型組、電針組、西藥組實(shí)驗(yàn)兔腹腔巨噬細(xì)胞中SR-AI蛋白表達(dá)水平均上調(diào),差異具有統(tǒng)計(jì)學(xué)意義(P0.01);與模型組比較,電針組、西藥組實(shí)驗(yàn)兔SR-AI蛋白的表達(dá)水平均降低,差異具有統(tǒng)計(jì)學(xué)意義(P0.01);電針組與西藥組組間無顯著差異(P0.05)。3與空白組比較,模型組、電針組、西藥組實(shí)驗(yàn)兔腹腔巨噬細(xì)胞中SR-AIm RNA表達(dá)水平均上調(diào),差異具有統(tǒng)計(jì)學(xué)意義(P0.01);與模型組比較,電針組、西藥組實(shí)驗(yàn)兔SR-AIm RNA的表達(dá)水平均降低,差異具有統(tǒng)計(jì)學(xué)意義(P0.01)電針組與西藥組組間無顯著差異(P0.05)。結(jié)論:1電針內(nèi)關(guān)、關(guān)元、足三里穴可改善動(dòng)脈粥樣硬化模型兔動(dòng)脈粥樣斑塊以及調(diào)節(jié)血脂紊亂情況。2電針內(nèi)關(guān)、關(guān)元、足三里穴可抑制動(dòng)脈粥樣硬化模型兔腹腔巨噬細(xì)胞中SR-AI蛋白和m RNA的表達(dá),這可能是針刺防治AS的又一機(jī)制。
[Abstract]:Objective: to observe the effect of electroacupuncture at "Zusanli", "Neiguan" and "Guanyuan" on the changes of blood lipid and the expression of SR-AI protein and m RNA in peritoneal macrophages of atherosclerotic rabbits. To provide a new experimental basis for the mechanism of acupuncture anti-atherosclerosis. Materials and methods: after 26 healthy New Zealand rabbits were fed adaptively for one week, 7 rabbits were randomly selected as the blank group to feed the standard rabbit feed, and the remaining 19 rabbits were fed the high-fat diet for the model group. After 4 weeks of high fat feeding, the common carotid artery injury was performed in the model group, and the model of high fat feeding for 4 Zhou Jianli was continued. One rabbit was randomly selected from the blank group and the model group to make pathological sections of the common carotid artery. The pathological morphology was observed by HE staining to determine whether the model was successful or not. Then the remaining 18 rabbits in the model group were randomly divided into three groups: model group, western medicine group, electroacupuncture group, 6 rabbits in each group. The electroacupuncture group was treated with "Neiguan", "Zusanli" and "Guanyuan", and the western medicine group was treated with Atto vastatin calcium tablets. After 4 weeks of treatment, the pathological changes of the common carotid artery were observed, and the contents of cholesterol, triglyceride, low density lipoprotein (LDL) and high density lipoprotein (HDL) were detected. The expression of SR-AIm RNA in peritoneal macrophages of as experimental rabbits was detected by RT-PCR technique. The expression of SR-AI protein was detected by Western-blot method. Results compared with the control group, the content of HDL in the model group, electroacupuncture group and western medicine group all increased, but the content of HDL decreased, the difference was statistically significant (P0.01), compared with the model group, the content of HDL in the electroacupuncture group and the western medicine group were all lower than that in the model group. There was no significant difference between the electroacupuncture group and the western medicine group. Compared with the blank group, the expression of SR-AI protein in the peritoneal macrophages of model group, electroacupuncture group and western medicine group were all up-regulated. Compared with the model group, the expression of SR-AI protein in the electroacupuncture group and the western medicine group were all decreased, the difference was statistically significant (P 0.01), there was no significant difference between the electroacupuncture group and the western medicine group, and there was no significant difference between the electroacupuncture group and the control group, the model group. The expression of SR-AIm RNA in peritoneal macrophages of experimental rabbits in electroacupuncture group and western medicine group were all up-regulated, the difference was statistically significant (P 0.01), compared with model group, the expression level of SR-AIm RNA in electroacupuncture group and western medicine group were all lower than that in model group. There was no significant difference between electroacupuncture group and western medicine group. Conclusion the electroacupuncture of Neiguan, Guan Yuan and Zusanli can improve the atherosclerotic plaque and regulate the disorder of blood lipids in rabbit model of atherosclerosis. 2 electroacupuncture at Neiguan, Guanyuan, and Guanyuan can improve the atherosclerotic plaque in rabbits. Zusanli point can inhibit the expression of SR-AI protein and m RNA in peritoneal macrophages of atherosclerotic rabbits, which may be another mechanism of acupuncture to prevent and treat as.
【學(xué)位授予單位】：遼寧中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 金穎;;穴位注射東莨菪堿和維生素B_6治療老年腦動(dòng)脈硬化性眩暈患者效果觀察[J];中國鄉(xiāng)村醫(yī)藥;2017年02期

2 周亮;盛飛;湯偉;郁志華;陳久林;;活血愈脈方穴位敷貼涌泉穴治療頸動(dòng)脈粥樣硬化癥的臨床研究分析[J];中國中醫(yī)藥現(xiàn)代遠(yuǎn)程教育;2016年21期

3 成澤東;陳以國;李曉梅;覃佩蘭;梁繁榮;榮培晶;;電針“內(nèi)關(guān)”穴對急性心肌缺血小鼠心肌組織氯離子通道蛋白表達(dá)的影響[J];針刺研究;2016年05期

4 黃暢;崔瑩雪;劉鈞天;劉耀萌;韓麗;于夢蕓;趙百孝;;艾灸及艾煙對載脂蛋白E基因敲除小鼠血清MDA、SOD水平的影響[J];世界中醫(yī)藥;2016年08期

5 崔瑩雪;趙百孝;劉鈞天;黃玉海;;艾灸及艾煙對ApoE~(-/-)小鼠血脂、肝臟病理形態(tài)及肝臟CD36、ABCA1表達(dá)的影響[J];上海針灸雜志;2016年08期

6 秦?zé)?;針灸治療頸動(dòng)脈硬化臨床研究[J];現(xiàn)代中醫(yī)藥;2016年04期

7 鄧敏;徐珊珊;孫亦農(nóng);龔美蓉;;穴位埋線對單純性肥胖小鼠減脂作用及脂肪組織炎癥相關(guān)因子的影響[J];時(shí)珍國醫(yī)國藥;2016年05期

8 曾彥平;汪韜;曾永紅;謝凡;李潔華;朱洪翔;;電針配合穴位按摩治療頸動(dòng)脈硬化的臨床研究[J];心血管康復(fù)醫(yī)學(xué)雜志;2016年02期

9 朱斯洪;馮秋玲;;耳穴貼壓聯(lián)合行為生活方式干預(yù)對高脂血癥患者的影響[J];中國民族民間醫(yī)藥;2016年06期

10 王鳴;劉志誠;徐斌;;電針聯(lián)合撳針治療肥胖并發(fā)高脂血癥胃腸腑熱型患者良性作用的研究[J];時(shí)珍國醫(yī)國藥;2015年10期

相關(guān)會(huì)議論文 前1條

1 岳增輝;何新群;常小榮;袁建菱;郁保生;符凌;張亮;商李超;;隔藥餅灸對動(dòng)脈粥樣硬化兔主動(dòng)脈TLR4的影響[A];2011中國針灸學(xué)會(huì)年會(huì)論文集（摘要）[C];2011年

相關(guān)博士學(xué)位論文 前2條

1 趙釗;隔藥餅灸對高脂血癥合并AS兔膽固醇逆轉(zhuǎn)運(yùn)SR-B1介導(dǎo)途徑及炎癥反應(yīng)的影響[D];湖南中醫(yī)藥大學(xué);2016年

2 金益煥;電針對APOE~(-/-)小鼠ABCAI、NF-КB表達(dá)的影響[D];南京中醫(yī)藥大學(xué);2015年

相關(guān)碩士學(xué)位論文 前10條

1 謝濤;隔藥餅灸對動(dòng)脈粥樣硬化兔易損斑塊TLR2/4-IRF3信號通路調(diào)控膽固醇逆轉(zhuǎn)運(yùn)的影響[D];湖南中醫(yī)藥大學(xué);2015年

2 曾粒;基于直接藍(lán)71免洗脫染色的蛋白質(zhì)免疫印跡內(nèi)參方法學(xué)研究[D];重慶醫(yī)科大學(xué);2014年

3 石秀芳;手足口病RT-PCR和FQ-PCR檢測方法比較及亳州市流行特征研究[D];安徽醫(yī)科大學(xué);2013年

4 林婉敏;溫針灸治療脾虛痰濕型高脂血癥的臨床研究[D];廣州中醫(yī)藥大學(xué);2011年

5 蘭天飛;靈芝多糖拮抗黑色素瘤細(xì)胞上清對腹腔巨噬細(xì)胞免疫功能抑制作用的研究[D];承德醫(yī)學(xué)院;2011年

6 何新群;隔藥餅灸抑制TLR4延遲動(dòng)脈粥樣硬化形成機(jī)制的研究[D];湖南中醫(yī)藥大學(xué);2010年

7 葉煜婉;穴位埋藥線對腦缺血大鼠纖溶酶系統(tǒng)的影響及對腦微血管的保護(hù)作用[D];湖北中醫(yī)學(xué)院;2009年

8 賈君君;手針與電針豐隆穴調(diào)節(jié)高脂血癥大鼠血脂等指標(biāo)的對比研究[D];北京中醫(yī)藥大學(xué);2007年

9 梁東明;雌雄激素對雄性大鼠和男性血脂代謝影響的實(shí)驗(yàn)與臨床研究[D];第四軍醫(yī)大學(xué);2007年

10 肖玲;電針對腦出血大鼠基質(zhì)金屬蛋白酶表達(dá)影響的實(shí)驗(yàn)研究[D];湖北中醫(yī)學(xué)院;2005年

，

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