本文選題：心房顫動(dòng) + 熱休克蛋白27�。� 參考：《遼寧中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:基于心肝相關(guān)理論探究炎性因子熱休克蛋白27(HSP27)及C反應(yīng)蛋白(CRP)在不同年齡組心房顫動(dòng)(房顫)患者中血清的表達(dá)及其臨床意義。材料與方法:從“肝”辯證,收集滿足肝血虧虛、心失所養(yǎng);或肝陽上亢、上擾心神;或肝郁氣滯、心脈痹阻三種證型并符合西醫(yī)診斷標(biāo)準(zhǔn)的老年房顫患者90例,按不同年齡段分為A組(60-69歲)30例,B組(70-79歲)30例,C組(80-89歲)30例,選取24小時(shí)動(dòng)態(tài)心電圖正�；颊�30例為正常對(duì)照組,平均年齡(67±6.8)歲。抽取房顫患者發(fā)作當(dāng)天靜脈血4ml,置于EDTA抗凝加抑肽酶50U試管中,并于冰盒內(nèi)保存,2h內(nèi)將血標(biāo)本放于4℃3000rpm離心機(jī)離心10min后取血清,最后保存于-80℃低溫冰箱中。采用酶聯(lián)免疫法(ELISA法)分別測(cè)定CRP及HSP27濃度,比較各組間血清CRP、HSP27水平差異。結(jié)果:1.A組患者血清中HSP27的濃度為0.499±0.071mg/L,B組患者血清中HSP27的濃度為0.466±0.054mg/L,C組患者血清中HSP27的濃度為0.426±0.079mg/L,對(duì)照組血清中HSP27濃度為0.393±0.029mg/L。不同年齡組患者血清中HSP27濃度與對(duì)照組血清中HSP27濃度差異有統(tǒng)計(jì)學(xué)意義,各年齡組患者血清中HSP27濃度差異也均具有統(tǒng)計(jì)學(xué)意義,且年齡與HSP27濃度呈負(fù)相關(guān)。2.A組患者血清中CRP的濃度為0.489±0.053mg/L,B組患者血清中CRP的濃度為0.456±0.065mg/L,C組患者血清中CRP的濃度為0.427±0.036mg/L,對(duì)照組血清中CRP濃度為0.397±0.069mg/L。不同年齡組患者血清中CRP濃度與對(duì)照組血清中CRP濃度差異有統(tǒng)計(jì)學(xué)意義,各年齡組患者血清中CRP濃度差異也均具有統(tǒng)計(jì)學(xué)意義,且年齡與CRP濃度呈負(fù)相關(guān)。結(jié)論:本實(shí)驗(yàn)表明,房顫的發(fā)生可能與炎癥有關(guān),炎性因子HSP27及CRP水平在房顫患者中的表達(dá)較對(duì)照組更有意義,其中HSP27的表達(dá)意義尤其突出,認(rèn)為可作為預(yù)測(cè)房顫發(fā)生的指標(biāo)。年齡與CRP和HSP27的表達(dá)呈負(fù)性相關(guān),說明年齡越小,CRP與HSP27對(duì)房顫風(fēng)險(xiǎn)的預(yù)測(cè)越有意義。
[Abstract]:Aim: to explore the expression and clinical significance of inflammatory factor heat shock protein 27 (HSP27) and C-reactive protein (CRP) in patients with atrial fibrillation (AF) in different age groups. Materials and methods: from the dialectical view of "liver", 90 cases of senile atrial fibrillation were collected to satisfy the deficiency of liver and blood, heart loss of nourishment, hyperactivity of liver-yang and disturbance of heart and mind, or three types of syndrome of stagnation of liver-qi and blockage of heart and veins, which met the diagnostic criteria of western medicine. According to different age groups, 30 patients were divided into group A (n = 30) and group B (n = 30). Group C (n = 30) were divided into group C (n = 30) and group C (n = 30). 30 patients with normal 24-hour ambulatory electrocardiogram were selected as normal control group, with an average age of 67 鹵6.8 years. The venous blood was extracted from the patients with atrial fibrillation on the same day and placed in the EDTA anticoagulant and aprotinin 50U test tube. The blood samples were stored in the Yu Bing box for 2 hours after centrifuged 10min with 4 鈩，

本文編號(hào)：1983054