發(fā)布時(shí)間：2018-06-05 08:37

  本文選題：藏藥 + 翼首草�。� 參考：《中國藥房》2017年07期

【摘要】：目的:建立測(cè)定藏藥翼首草不同藥用部位中齊墩果酸和熊果酸含量的方法,并比較其不同部位之間的含量差異。方法:采用超高效液相色譜-光電二極管陣列檢測(cè)器(UPLC-PDA)測(cè)定翼首草不同藥用部位(全草、地上部位和地下部位)齊墩果酸和熊果酸含量。色譜柱為Acquity UPLC HSS T3(150 mm×2.1 mm,1.8μm);流動(dòng)相為甲醇-0.1 mol/L乙酸銨溶液(88∶12,V/V),流速為0.2 m L/min;檢測(cè)波長為210 nm;柱溫為30℃;進(jìn)樣量為5μL。結(jié)果:齊墩果酸和熊果酸的檢測(cè)質(zhì)量濃度線性范圍分別為10.65~1 065μg/m L(r=0.999 6)、18.8~1 880μg/m L(r=0.999 4),平均加樣回收率分別為96.95%(RSD=1.24%,n=9)、98.12%(RSD=2.13%,n=9),精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD均小于3%。不同藥用部位的齊墩果酸和熊果酸含量的高低整體趨勢(shì)為地上部位全草地下部位;翼首草全草中齊墩果酸和熊果酸平均總量為0.35%,地上部位中為0.56%,地下部位中為0.09%。結(jié)論:建立的方法快速、準(zhǔn)確可靠、重復(fù)性好,適合于藏藥翼首草不同藥用部位中齊墩果酸和熊果酸含量的測(cè)定。翼首草地上部位中齊墩果酸和熊果酸含量較全草、地下部位高,建議使用地上部位入藥。
[Abstract]:Objective: to establish a method for the determination of oleanolic acid and ursolic acid in different parts of Pterygium hirsutum, and to compare the content differences between different parts of oleanolic acid and ursolic acid. Methods: the contents of oleanolic acid and ursolic acid in different medicinal parts (whole grass, ground part and underground part) were determined by UPLC-PDAs by ultra-high performance liquid chromatography-photodiode array detector (UPLC-PDAs). The mobile phase consisted of methanol-0.1 mol/L ammonium acetate solution with a flow rate of 0.2ml / min, a column temperature of 30 鈩，

本文編號(hào)：1981362