本文選題：楓楊 + 類風(fēng)濕關(guān)節(jié)炎�。� 參考：《湖北民族學(xué)院》2016年碩士論文

【摘要】：目的:研究楓楊對(duì)類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞的作用,證實(shí)楓楊祛風(fēng)除濕、消腫止痛的功效,為治療痹癥發(fā)掘新藥。方法:制備楓楊萃取物的藥液,用不同時(shí)間、不同濃度的藥液刺激類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞。用CCK-8法、流式細(xì)胞術(shù)檢測(cè)楓楊萃取物對(duì)類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞起到的作用。Western-Blot檢測(cè)楓楊萃取物培養(yǎng)的類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞的caspase-3和caspase-8蛋白的表達(dá)。ELISA檢測(cè)楓楊萃取物培養(yǎng)的類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞上清中IL-8的含量。結(jié)果:用不同濃度楓楊萃取物干預(yù)類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞,發(fā)現(xiàn)楓楊能夠抑制細(xì)胞增殖,且呈時(shí)間和劑量依賴性(P0.05)。采用流式細(xì)胞儀檢測(cè)楓楊萃取物誘導(dǎo)類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞凋亡情況,發(fā)現(xiàn)該藥物能夠有效誘導(dǎo)細(xì)胞凋亡(P0.01),2mg/ml藥物濃度時(shí)凋亡細(xì)胞率為19.59%,當(dāng)藥物濃度從2.5mg/ml升高至3.33mg/ml時(shí),細(xì)胞總凋亡率從42.03%增加到78.42%。用不同濃度楓楊萃取物培養(yǎng)的類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞,高劑量濃度組caspase-3和caspase-8蛋白表達(dá)上調(diào)(P0.05)。1.25mg/ml藥物濃度時(shí)楓楊萃取物培養(yǎng)的類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞上清中IL-8的含量明顯降低,且呈時(shí)間依賴性(P0.05)。結(jié)論:楓楊能抑制類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞的增殖,誘導(dǎo)類風(fēng)濕關(guān)節(jié)炎成纖維滑膜細(xì)胞凋亡,并降低IL-8生成。有必要對(duì)其機(jī)制及其對(duì)類風(fēng)濕關(guān)節(jié)炎治療的臨床效果做進(jìn)一步研究。
[Abstract]:Objective: To study the effect of maple poplar on rheumatoid arthritis fibrosynovial cells, verify the effect of maple poplar to dispel wind and dehumidification, eliminate swelling and relieve pain, explore new medicine for the treatment of arthralgia syndrome. Method: preparation of the liquid of maple poplar extract, using different time and different concentration of liquid to stimulate rheumatoid arthrosis fibroblast synovial cells. CCK-8 method and flow cytometry are used. The effect of maple poplar extract on rheumatoid arthritis fibroblast synovial cells.Western-Blot detection of Caspase-3 and caspase-8 protein expression of rheumatoid arthritis fibroblast synovial cells cultured by Maple Yang Cui extract.ELISA detection of IL-8 content in the supernatant of rheumatoid arthritis fibroblast synovial cells cultured by Maple poplar extract. Fruit: using different concentrations of maple poplar extract to interfere with the fibroblast synovial cells of rheumatoid arthritis, it was found that maple poplar could inhibit cell proliferation and was time and dose dependent (P0.05). Flow cytometry was used to detect the withering of rheumatoid arthritis fibrosynovial cells induced by Maple poplar extract, and it was found that the drug could effectively induce cell withering. Death (P0.01), the apoptotic cell rate of 2mg/ml drug concentration was 19.59%. When the drug concentration increased from 2.5mg/ml to 3.33mg/ml, the total cell apoptosis rate increased from 42.03% to 78.42%. with different concentrations of maple poplar extract for rheumatoid arthritis fibroblast synovial cells, and the high dose concentration group caspase-3 and caspase-8 protein expression up (P0.05).1.25mg/ The content of IL-8 in the supernatant of rheumatoid arthritis fibrosynovial cells cultured by poplar extract at mL concentration significantly decreased and was time dependent (P0.05). Conclusion: Maple poplar can inhibit the proliferation of fibrous synovial cells of rheumatoid arthritis, induce apoptosis of fibroblast synovial cells in rheumatoid arthritis and reduce the formation of IL-8. It is necessary to Its mechanism and its clinical effect in the treatment of rheumatoid arthritis are further studied.
【學(xué)位授予單位】：湖北民族學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R259

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 張譽(yù)丹;徐俊;袁德培;袁林;;土家醫(yī)治療類風(fēng)濕關(guān)節(jié)炎研究現(xiàn)狀[J];湖北民族學(xué)院學(xué)報(bào)(醫(yī)學(xué)版);2018年01期

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本文編號(hào)：1934949