本文選題：失眠 + 電針��； 參考：《成都中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的：觀察藍(lán)斑去甲腎上腺素在失眠中的作用,電針“神門”、“三陰交”對(duì)失眠模型大鼠睡眠、焦慮抑郁狀態(tài)以及中樞、血漿兒茶酚胺類神經(jīng)遞質(zhì)的影響,探索電針治療失眠的交感-腎上腺髓質(zhì)系統(tǒng)機(jī)制。方法：將健康SPF級(jí)SD大鼠以體重、高架十字迷宮實(shí)驗(yàn)、鼠尾懸掛實(shí)驗(yàn)數(shù)據(jù)為基準(zhǔn),篩選合格動(dòng)物,并將其隨機(jī)分為空白組、假注射組、NE模型組、NE模型電針組。NE模型組和NE模型電針組進(jìn)行側(cè)腦室微量注射去甲腎上腺素,假注射組插入微量注射針頭但不注射藥物,空白組常規(guī)飼養(yǎng)。側(cè)腦室注射后,各組進(jìn)行24小時(shí)自發(fā)活動(dòng)檢測、高架十字迷宮實(shí)驗(yàn)以及鼠尾懸掛實(shí)驗(yàn)。檢測后,NE模型電針組行“神門”、“三陰交”電針治療,連續(xù)治療4天,其余各組同步抓取固定不治療。電針治療后,各組先進(jìn)行24小時(shí)自發(fā)活動(dòng)、高架十字迷宮實(shí)驗(yàn)、鼠尾懸掛實(shí)驗(yàn)檢測,再進(jìn)行丘腦、腦干去甲腎上腺素及血漿去甲腎上腺素、腎上腺素、多巴胺含量檢測。結(jié)果：(1)各組基線情況：空白組、假注射組、NE模型組、NE模型電針組大鼠在體重、鼠尾懸掛實(shí)驗(yàn)靜止時(shí)間以及高架十字迷宮實(shí)驗(yàn)OE%、OT%方面,組間比較無統(tǒng)計(jì)學(xué)差異(P0.05)。(2)側(cè)腦室注射后行為學(xué)情況：側(cè)腦室注射后,各組大鼠在鼠尾懸掛實(shí)驗(yàn)靜止時(shí)間方面,組間差異無統(tǒng)計(jì)學(xué)意義(P0.05)；而在24小時(shí)自發(fā)活動(dòng)、高架十字迷宮實(shí)驗(yàn)OE%和OT%方面,組間差異均具有統(tǒng)計(jì)學(xué)差異(P0.05)。差異具體表現(xiàn)為：①24小時(shí)自發(fā)活動(dòng)：NE模型組、NE模型電針組白晝活動(dòng)量與空白組、假注射組相較顯著增加(P0.05)、夜晚活動(dòng)量顯著減少(P0.05),假注射組白晝活動(dòng)量、夜晚活動(dòng)量與空白組相較無明顯差異(P0.05)；②高架十字迷宮實(shí)驗(yàn)：NE模型組、NE模型電針組OE%和0T%與空白組、假注射組相較顯著降低(P0.05),假注射組OE%和OT%與空白組相較無明顯改變(P0.05)。(3)電針治療后行為學(xué)情況：電針治療后,各組大鼠在鼠尾懸掛實(shí)驗(yàn)靜止時(shí)間方面,組間差異無統(tǒng)計(jì)學(xué)意義(P0.05)；而在24小時(shí)自發(fā)活動(dòng)、高架十字迷宮實(shí)驗(yàn)OE%和OT%方面,組間差異均具有統(tǒng)計(jì)學(xué)差異(P0.05)。差異具體表現(xiàn)為：①24小時(shí)自發(fā)活動(dòng)：NE模型組白晝活動(dòng)量與空白組、假注射組相較顯著增加(P0.05)、夜晚活動(dòng)量顯著減少(P0.05),NE模型電針組白晝活動(dòng)量與NE模型組相較明顯減少(P0.05)、夜晚活動(dòng)量明顯增加(P0.05),NE模型電針組白晝活動(dòng)量、夜晚活動(dòng)量與空白組、假注射組相較無明顯差異(P0.05),’假注射組白晝活動(dòng)量、夜晚活動(dòng)量與空白組相較無明顯差異(P0.05)；②高架十字迷宮實(shí)驗(yàn)：NE模型組OE%和OT%與空白組、假注射組相較明顯降低(P0.05),NE模型電針組OE%和OT%與NE模型組相較明顯升高(P0.05),NE模型電針組OE%和OT%與空白組、假注射組相較無明顯差異(P0.05),假注射組OE%和OT%與空白組相較無明顯差異(P0.05)。(4)電針治療后神經(jīng)遞質(zhì)情況：電針治療后,各組大鼠在丘腦、腦干去甲腎上腺素含量,血漿去甲腎上腺素、腎上腺素、多巴胺含量方面,組間差異均具有統(tǒng)計(jì)學(xué)差異(P0.05)。差異具體表現(xiàn)為：①丘腦、腦干去甲腎上腺素含量：NE模型組與空白組、假注射組相較明顯增加(P0.05),NE模型電針組與NE模型組相較明顯減少(P0.05),NE模型電針組與空白組、假注射組相較無明顯差異(P0.05),假注射組與空白組相較無明顯差異(P0.05);②血漿去甲腎上腺素、腎上腺素、多巴胺含量：NE模型組與空白組、假注射組相較明顯升高(P0.05),NE模型電針組與NE模型組相較明顯減低(P0.05),NE模型電針組與空白組、假注射組相較無明顯差異(P0.05),假注射組與空白組相較無明顯差異(P0.05)。結(jié)論：側(cè)腦室微量注射去甲腎上腺素建立失眠模型具有可行性,模型大鼠具有交感-腎上腺髓質(zhì)系統(tǒng)興奮、睡眠障礙以及焦慮等類似人類失眠的表現(xiàn),藍(lán)斑去甲腎上腺素可興奮交感-腎上腺髓質(zhì)系統(tǒng)引發(fā)失眠；電針神門、三陰交通過調(diào)節(jié)藍(lán)斑去甲腎上腺素含量、改善交感-腎上腺髓質(zhì)系統(tǒng)功能狀態(tài)治療失眠。
[Abstract]:Objective: To observe the effect of norepinephrine on insomnia, the effect of electroacupuncture "Shen gate" and "Sanyinjiao" on sleep, anxiety and depression and the center, plasma catecholamine neurotransmitters in the rat model of insomnia model, and explore the mechanism of the sympathetic adrenomedullary system in the treatment of insomnia by electroacupuncture. Methods: the body of healthy SPF SD rats is in body. Heavy, elevated cross labyrinth test and rat tail suspension test data were used to screen qualified animals, and they were randomly divided into blank group, false injection group, NE model group, NE model electroacupuncture group.NE model group and NE model electroacupuncture group for lateral ventricle microinjection of norepinephrine, false injection group inserted micro injection needles but no drug injection, blank group. After the routine feeding, after the injection of the lateral ventricle, 24 hours of spontaneous activity detection, the viaduct test and the tail suspension test were carried out. After the test, the NE model electroacupuncture group was treated with the "Shen gate", "Three Yin cross" electroacupuncture treatment, and the other groups were treated for 4 days. The rest of the acupuncture groups were not treated at the same time. After the electroacupuncture treatment, 24 hours of spontaneous activity was carried out in each group. An elevated cross labyrinth test, a rat tail suspension test, and then a test of thalamus, norepinephrine and plasma norepinephrine, adrenaline and dopamine in the brain stem. Results: (1) the baseline conditions of each group: blank group, false injection group, NE model group, NE model electroacupuncture group in body weight, stern suspension experiment time and Viaduct The cross maze test OE%, OT%, there was no statistical difference between groups (P0.05). (2) after lateral ventricle injection, after injection of the lateral ventricle, there was no significant difference between the groups in the rest of the rat tail suspension experiment (P0.05), but in the 24 hour spontaneous activity, OE% and OT% in the elevated cross maze test, the difference between the groups There were statistical differences (P0.05). The differences were as follows: (1) 24 hours of spontaneous activity: the NE model group, the day activity of the NE model electroacupuncture group and the blank group, the false injection group increased significantly (P0.05), the night activity decreased significantly (P0.05), the false injection group was alive in the daytime, and there was no significant difference between the night activity and the blank group (P0.05). (2) the elevated cross maze test: NE model group, NE model electroacupuncture group OE% and 0T% and blank group, false injection group decreased significantly (P0.05), OE% and OT% in the false injection group were not significantly changed (P0.05). (3) the behavior after electroacupuncture treatment: after electroacupuncture treatment, the rest time of rats in rats tail suspension experiment, the difference between groups There was no statistical significance (P0.05), but in 24 hours of spontaneous activity, the differences in OE% and OT% were statistically different (P0.05). The differences were as follows: (1) 24 hours of spontaneous activity: the daytime activity in the NE model group and the blank group, the false injection group increased significantly (P0.05), and the activity at night decreased significantly (P0.05 In the NE model, the daytime activity of the electroacupuncture group decreased significantly (P0.05), the night activity increased significantly (P0.05), the daytime activity of the NE model electroacupuncture group, the night activity and the blank group, and the false injection group had no significant difference (P0.05), "the white day activity in the false injection group, and the night activity volume and the blank group had no significant difference (P0.05). (2) the elevated cross labyrinth test: the NE model group OE% and OT% and the blank group, the false injection group decreased significantly (P0.05), the OE% and OT% in the NE model group were significantly higher than the NE model group (P0.05), the NE model electroacupuncture group was OE% and OT% and blank groups, and the false injection group had no obvious difference. The difference (P0.05). (4) the neurotransmitter after electroacupuncture treatment: after electroacupuncture treatment, the differences in the content of thalamus, norepinephrine, norepinephrine, epinephrine and dopamine in the rats were all statistically different (P0.05). The difference specific manifestations were: (1) thalamus, brain stem norepinephrine content: NE model The group and the blank group, the false injection group increased significantly (P0.05), the NE model electroacupuncture group and the NE model group decreased significantly (P0.05). The NE model electroacupuncture group and the blank group, the false injection group were not significantly different (P0.05), the false injection group was not significantly different from the blank group (P0.05); (2) the plasma norepinephrine, adrenaline, dopamine content: NE The model group and the blank group, the false injection group increased significantly (P0.05), the NE model electroacupuncture group was significantly lower than the NE model group (P0.05). The NE model electroacupuncture group and the blank group, the false injection group were not significantly different (P0.05), the false injection group was not significantly different from the blank group (P0.05). Conclusion: the side ventricle microinjection of norepinephrine to establish the loss of norepinephrine. Sleep model is feasible, model rats have sympathetic adrenal medullary system excitement, sleep disorder and anxiety, such as the performance of human insomnia, blue norepinephrine can stimulate the sympathetic adrenal medulla system to cause insomnia; Electroacupuncture of the God gate, three yin traffic regulation of the locus methadinephrine content, the improvement of sympathetic adrenomedullary medulla The functional state of the system is used to treat insomnia.

【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R246.6

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