本文選題：激素性股骨頭壞死 + 骨碎補(bǔ)總黃酮�。� 參考：《河南中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:本課題通過(guò)觀察骨碎補(bǔ)總黃酮對(duì)新西蘭大白兔激素性股骨頭壞死血清甘油三酯、總膽固醇及各組動(dòng)物脂肪細(xì)胞平均直徑、骨小梁面積的影響,研究骨碎補(bǔ)總黃酮治療激素性股骨頭壞死的作用效果和作用機(jī)理。方法:實(shí)驗(yàn)采用新西蘭大白兔30只(雄雌各半)作為研究對(duì)象,建立激素性股骨頭壞死模型后,并于造模成功后第一天空白、模型組給予生理鹽水6ml·k g-1灌胃,實(shí)驗(yàn)組按照35mg·kg-1給予骨碎補(bǔ)總黃酮溶液(6 g·L~(-1))。在灌胃8周之后,檢測(cè)各組動(dòng)物血清甘油三酯、總膽固醇的變化情況,解剖出股骨頭并切片光鏡下計(jì)算脂肪細(xì)胞平均直徑、骨小梁面積,然后進(jìn)行組間比較。結(jié)果:血清甘油三酯、總膽固醇的測(cè)定:空白組與模型組血清甘油三酯、總膽固醇相比,差異均有顯著統(tǒng)計(jì)學(xué)意義(P0.05),表明通過(guò)大劑量的激素應(yīng)用后模型組家兔的血清甘油三酯明顯升高,總膽固醇明顯升高。實(shí)驗(yàn)組與模型組血清甘油三酯、總膽固醇比較,差異均有顯著性差異(P0.05),實(shí)驗(yàn)組與空白組相比,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),提示用骨碎補(bǔ)總黃酮干預(yù)后,血清甘油三酯、總膽固醇水平基本可以降低到正常水平。脂肪細(xì)胞平均直徑的測(cè)定:模型組與空白組、實(shí)驗(yàn)組相比,脂肪細(xì)胞平均直徑明顯增大,差異均有顯著性統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組與空白組比較,脂肪細(xì)胞平均直徑無(wú)明顯變化,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。骨小梁面積模型組與空白組、實(shí)驗(yàn)組比較,模型組骨小梁面積明顯減少,組間差異均有統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組和空白組比較,兩組細(xì)胞中骨小梁面積無(wú)明顯變化,組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:骨碎補(bǔ)總黃酮可以調(diào)控血清甘油三酯、總膽固醇的含量,改善脂肪細(xì)胞平均直徑,有改善脂肪代謝紊亂,促進(jìn)堆積脂肪代謝的作用;能夠刺激骨小梁的生成,最終實(shí)現(xiàn)防治激素性股骨頭壞死的目的,為臨床治療本病提供理論依據(jù)。
[Abstract]:Objective: to observe the effects of total flavonoids of Fructus fragmentosa on serum triglyceride, total cholesterol, mean diameter of adipocytes and trabeculae area of steroid-induced femoral head necrosis in New Zealand white rabbits. Objective: to study the effect and mechanism of total flavonoids in the treatment of steroid-induced osteonecrosis of femoral head. Methods: thirty New Zealand white rabbits (male and half female) were used to establish steroid-induced femoral head necrosis model. The model group was given normal saline 6ml kg-1 intragastrically on the first day after the establishment of the model. The experimental group was given 6 g of total flavonoids solution according to 35mg kg-1. After 8 weeks of gastric perfusion, the changes of serum triglyceride and total cholesterol were measured, the femoral head was dissected and the mean diameter of adipocytes and trabecular area of bone were calculated under light microscope, and then the comparison was made between the groups. Results: determination of serum triglyceride and total cholesterol: serum triglyceride and total cholesterol were compared between blank group and model group. The results showed that the serum triglyceride and total cholesterol in the model group were significantly higher than those in the control group. There were significant differences in serum triglyceride and total cholesterol between the experimental group and the model group (P 0.05), but there was no significant difference between the experimental group and the blank group (P 0.05). Total cholesterol levels can be basically reduced to normal levels. Measurement of mean diameter of adipocytes: the mean diameters of adipocytes in model group and blank group were significantly larger than those in control group (P 0.05), but there was no significant change in average diameter of adipocytes in experimental group and blank group. The difference was not statistically significant (P 0.05). The trabecular area in the model group was significantly decreased compared with the blank group, and the difference between the two groups was statistically significant (P 0.05), but there was no significant change in the trabecular area between the experimental group and the blank group. There was no significant difference between the two groups (P 0.05). Conclusion: the total flavonoids can regulate the content of serum triglyceride and total cholesterol, improve the average diameter of adipocytes, improve the disorder of fat metabolism, promote the accumulation of fat metabolism, and stimulate the formation of trabeculae. Finally, the purpose of prevention and treatment of hormone-induced femoral head necrosis is achieved, which provides a theoretical basis for the clinical treatment of this disease.
【學(xué)位授予單位】：河南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R274.9

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