本文選題：九龍盤 + 3β-angeloyloxy-7-epifutronolide�。� 參考：《廣西中醫(yī)藥大學》2016年碩士論文

【摘要】：目的:研究壯藥九龍盤的主要化學成分及其抗炎活性,為其藥效物質(zhì)基礎(chǔ)研究和開發(fā)利用提供科學依據(jù)。方法:以乙醇滲漉法提取九龍盤藥材得到乙醇總提取物,總提物用系統(tǒng)溶劑法分離,得到石油醚部位、乙酸乙酯部位、正丁醇部位、水部位和水不溶物。采用硅膠色譜、聚酰胺色譜、葡聚糖凝膠色譜、大孔吸附樹脂色譜、制備高效液相色譜、重結(jié)晶等分離方法對石油醚部位、乙酸乙酯部位和正丁醇部位的化學成分進行分離和純化,對單體化合物利用理化檢驗、MS、1H-NMR、13C-NMR、DEPT等波譜方法進行結(jié)構(gòu)鑒定。采用LD50測定法測試九龍盤各部位提取物的急性毒性,并通過小鼠耳腫脹、肉芽腫、腹腔毛細血管通透性三個模型對其各部位提取物進行抗炎實驗研究。結(jié)果:從九龍盤中分離得到28個化合物,鑒定了其中22個化合物的結(jié)構(gòu),分別為2-十五烷酮(1),木栓酮(2),三十烷醇(3),二十八烷酸(4),β-谷甾醇(5),豆甾醇(6),鄰苯二酚(7),山崳酸(8),對甲氧基肉桂酸(11),3β-angeloyloxy-7-epifutronolide(13),6-羥基己酸(14),檸檬黃素(15),芥酸(17),3,3'-O-二甲基鞣花酸(18),β-胡蘿卜苷(19),沒食子酸(20),3-甲氧基槲皮素(22),4’-甲基-棉花素(23),圣草酚(24),沒食子酸乙酯(25),沒食子酸甲酯(26),阿福豆苷(28)。急性毒性研究測得九龍盤提取物各部位灌胃給藥對小鼠的LD50分別為58.25g/kg(總提物)、1181.01g/kg(石油醚部位)、645.65g/kg(乙酸乙酯部位)、591.56g/kg(正丁醇部位)。九龍盤各部位提取物均能明顯抑制小鼠腹腔毛細血管擴張;總提物、石油醚部位能明顯抑制二甲苯致小鼠耳廓腫脹的炎癥、小鼠棉球肉芽腫的生成;乙酸乙酯部位和正丁醇部位對二甲苯致小鼠耳廓腫脹的炎癥沒有明顯作用,但抑制小鼠棉球肉芽腫的生成的效果顯著。結(jié)論:化合物1~4、7、8、11、13~15、17、18、20、22~26、28共19個化合物為首次從該植物中分離得到,其中1~4、7、8、11、13~15、17、18、22~26、28共18個化合物為首次從該屬植物中分離得到。九龍盤各部位提取物均有一定的毒性,總提物和石油醚部位提取物對小鼠的急性、慢性炎癥均有較好的治療效果,乙酸乙酯部位和正丁醇部位提取物則對抑制小鼠的慢性炎癥效果更佳。
[Abstract]:Objective: to study the main chemical constituents and anti-inflammatory activity of Jiulong plate, and to provide scientific basis for the basic research and development of its pharmacodynamic substance. Methods: the total ethanol extract was extracted by ethanol percolation method, and the total extract was separated by systematic solvent method. The petroleum ether, ethyl acetate, n-butanol, water and water insoluble substances were obtained. Silica gel chromatography, polyamide chromatography, dextran gel chromatography, macroporous adsorption resin chromatography, preparation of high performance liquid chromatography and recrystallization were used to separate petroleum ether parts. The chemical constituents of ethyl acetate and n-butanol were isolated and purified. The structure of the monomers was identified by means of MS 1H-NMR-13C-NMRDPT and other spectroscopic methods. The acute toxicity of extracts from different parts of Jiulong disk was measured by LD50 method. The anti-inflammatory effects of the extracts were studied by three models of ear swelling granuloma and celiac capillary permeability in mice. Results: 28 compounds were isolated from Jiulong pan and 22 of them were identified. They are 2-pentadecanone (1), corkone (2), triaconol (3), 28 alkanoic acid (4), 尾 -sitosterol (5), lasosterol (6), catechol (7), docosanoic acid (8), p-methoxy cinnamic acid (113 尾 -angeloyloxy-7-epifutronolide) (133 尾 -angeloyloxy-7-epifutronolide) (133) -hydroxy-caproic acid (14), limonium (15), erucidic acid (173-) 3- O- dimethylanthocyanate (18), 尾 -hulucidic acid (113 尾 -angeloyloyloxy-3-hydroxycaproic acid). Turniferin 19, Gallic acid 20, 3-methoxy quercetin, methoxyquercetin, methyloxyquercetin, methoxyquercetin, chrysophenol, ethyl gallate, methoxyquercetin, methyl gallate, methoxyquercetin, methoxyquercetin. Acute toxicity study showed that the LD50 of mice treated by gavage was 58.25g / kg (total extract 1181.01g / kg) (petroleum ether extract 6455.65g / kg) (ethyl acetate 59.56g / kg). The extract of each part of Jiulong disk could obviously inhibit the abdominal capillary dilatation of mice, the total extract and petroleum ether could obviously inhibit the inflammation of auricle swelling induced by xylene and the formation of cotton ball granuloma in mice. Ethyl acetate and n-butanol had no obvious effect on ear swelling induced by xylene in mice, but could inhibit the formation of cotton ball granuloma in mice. Conclusion: a total of 19 compounds were isolated from the plant for the first time, and 18 compounds were isolated from this genus for the first time. The extracts from all parts of the Kowloon plate were toxic to a certain extent. The total extract and petroleum ether extract had a good therapeutic effect on acute and chronic inflammation in mice. The extract of ethyl acetate and butanol had better effect on inhibiting chronic inflammation in mice.
【學位授予單位】：廣西中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R29

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