發(fā)布時(shí)間：2018-05-03 14:30

  本文選題：經(jīng)皮穴位電刺激 + 一次性大強(qiáng)度運(yùn)動(dòng)��； 參考：《武漢體育學(xué)院》2016年碩士論文

【摘要】：目的:觀察經(jīng)皮穴位電刺激干預(yù)后大鼠心肌細(xì)胞自噬、凋亡情況以及PKCδ、PKCε表達(dá)的變化,比較一次性大強(qiáng)度運(yùn)動(dòng)和不同形式的經(jīng)皮穴位電刺激對(duì)大鼠心肌細(xì)胞的影響,探討二者對(duì)運(yùn)動(dòng)心臟的作用機(jī)制,為中醫(yī)傳統(tǒng)方法在運(yùn)動(dòng)醫(yī)學(xué)領(lǐng)域的應(yīng)用提供理論依據(jù)。方法:健康雄性Sprague-Dauley(SD)大鼠24只,體重210±10 g,兩月齡,隨機(jī)分為4組,每組6只:無負(fù)重運(yùn)動(dòng)組(E)、6%負(fù)重運(yùn)動(dòng)組(EG)、6%負(fù)重加中頻穴位電刺激預(yù)處理組(MEG)、6%負(fù)重加中頻穴位電刺激后處理組(AEG),大鼠的運(yùn)動(dòng)時(shí)間為2小時(shí)。因一般運(yùn)動(dòng)員在休賽期都會(huì)保持小強(qiáng)度的訓(xùn)練,故本文將無負(fù)重小強(qiáng)度運(yùn)動(dòng)組(E)作為對(duì)照組。E組與EG組大鼠結(jié)束游泳運(yùn)動(dòng)后,立即斷頸處死獲取完整心臟;MEG組在游泳前半小時(shí)給予經(jīng)皮穴位電刺激預(yù)處理,游泳結(jié)束后立即斷頸處死,取完整心臟;AEG組在游泳后即刻給予穴位電刺激處理半小時(shí),處理完后取材。刺激穴位為足三里和內(nèi)關(guān)。通過HE染色、電鏡觀察心臟結(jié)構(gòu)改變,運(yùn)用免疫印跡試驗(yàn)(Western Blot)檢測心肌細(xì)胞LC3II/I、Beclin1、m TOR、PKCδ、PKCε表達(dá),Tunel檢測心肌細(xì)胞凋亡。結(jié)果:1.一次性大強(qiáng)度運(yùn)動(dòng)后,大鼠心肌組織和細(xì)胞的結(jié)構(gòu)形態(tài)產(chǎn)生一定變化,相比EG組經(jīng)皮穴位電刺激的MEG組和AEG組的心肌肌纖維排列、線粒體形態(tài)、細(xì)胞核形態(tài)都有一定程度的改善,MEG組的細(xì)胞狀態(tài)略好于AEG組,提示經(jīng)皮穴位電刺激預(yù)處理對(duì)大鼠心肌結(jié)構(gòu)保護(hù)的效果好于經(jīng)皮穴位電刺激后處理。2.EG組的凋亡率顯著高于E組(p0.01);MEG組的凋亡水平有顯著的下調(diào),AEG組的凋亡也出現(xiàn)下調(diào)(p0.01),但是下調(diào)的程度低于MEG組。Tunel檢測結(jié)果提示,一次性大強(qiáng)度運(yùn)動(dòng)能夠誘發(fā)心肌細(xì)胞的凋亡,而經(jīng)皮穴位電刺激能夠下調(diào)心肌組織的凋亡率,經(jīng)皮穴位電刺激預(yù)處理的作用效果優(yōu)于后處理。3.免疫印跡試驗(yàn)(Western Blot)結(jié)果顯示,EG組的LC3II/I的比值顯著高于E組(p0.05),提示大強(qiáng)度運(yùn)動(dòng)能夠激活自噬,MEG組的表達(dá)高于EG組(p0.05),提示經(jīng)皮穴位電刺激預(yù)處理能夠進(jìn)一步誘導(dǎo)自噬,對(duì)心臟起保護(hù)作用;AEG組的表達(dá)水平相較MEG組變化并不顯著(p0.05)。心肌細(xì)胞Beclin1的表達(dá)情況,與E組相比,EG組的表達(dá)略有下降,但降幅較小,MEG組Beclin1的表達(dá)水平提高明顯(p0.05),提示經(jīng)皮穴位電刺激預(yù)處理可以上調(diào)Beclin1的表達(dá),且具有顯著差異(p0.05);AEG組表達(dá)量亦出現(xiàn)上升,并具有顯著差異(p0.05),且AEG組和MEG組的表達(dá)水平差別不大,MEG組稍高一點(diǎn),但差距并不明顯(p0.05),提示經(jīng)皮穴位電刺激預(yù)處理與后處理對(duì)心肌Beclin1表達(dá)影響作用差別可能并不大。心肌細(xì)胞m TOR的表達(dá),與E組相比,EG組的表達(dá)顯著下調(diào)(p0.01),而MEG組和AEG組的表達(dá)量相較于E組和EG組也均顯著的下調(diào)(p0.01),AEG組的下降趨勢比MEG組更顯著(p0.01)。4.PKCδ的表達(dá)情況顯示,相對(duì)E組,EG組PKCδ的表達(dá)上調(diào);MEG和AEG組則呈下調(diào)趨勢(p0.05),提示一次大強(qiáng)度運(yùn)動(dòng)能夠上調(diào)PKCδ的表達(dá),經(jīng)皮穴位電刺激則能抑制其表達(dá)上調(diào)。心肌細(xì)胞PKCε的表達(dá)情況變化不明顯,各組基本都處于同一水平,沒有顯著性變化(p0.05)。結(jié)論:1、一次性大強(qiáng)度運(yùn)動(dòng)(6%負(fù)重)可以同時(shí)激活大鼠心肌細(xì)胞的自噬與凋亡。2、經(jīng)皮穴位電刺激可通過上調(diào)心肌細(xì)胞的自噬和抑制凋亡發(fā)生,對(duì)心肌進(jìn)行保護(hù)。3、經(jīng)皮穴位電刺激預(yù)處理下調(diào)心肌組織凋亡率的效果優(yōu)于后處理,兩者激活的自噬相關(guān)蛋白多無顯著差異。4、一次性大強(qiáng)度運(yùn)動(dòng)能上調(diào)PKCδ的表達(dá),經(jīng)皮穴位電刺激預(yù)處理和后處理通過抑制大鼠心肌細(xì)胞PKCδ的過表達(dá),發(fā)揮心肌保護(hù)作用。5、短周期一次性大強(qiáng)度運(yùn)動(dòng)和單次經(jīng)皮穴位電刺激對(duì)PKCε表達(dá)無顯著影響。
[Abstract]:Objective: To observe the autophagy, apoptosis and the changes of PKC Delta and PKC epsilon expression in rat cardiac myocytes after percutaneous acupoint electrical stimulation, and compare the effects of the single large intensity exercise and different forms of percutaneous acupoint electrical stimulation on the cardiac myocytes in rats, and explore the mechanism of the action of the two groups on the moving heart, and the traditional Chinese medicine method in the field of sports medicine. Methods: 24 healthy male Sprague-Dauley (SD) rats, weight 210 + 10 g, 2 month old, were randomly divided into 4 groups, and each group was randomly divided into 6 rats: no weight negative exercise group (E), 6% weight negative exercise group (EG), 6% load plus intermediate frequency acupoint electrical stimulation group (MEG), 6% weight negative plus intermediate frequency acupoint electrical stimulation group (AEG), and the exercise time of rats 2 hours. Because of the low intensity training of the general athletes during the period of the rest period, the small intensity exercise group (E) was used as the control group.E and the EG group to get the complete heart immediately after the end of the swimming exercise. In group MEG, the percutaneous point electrical stimulation was given half an hour before the swimming, and the neck was broken immediately after the swimming end. Death, the whole heart was taken, group AEG was given a half hour after the acupoint electric stimulation immediately after swimming. The stimulation points were Zusanli and Neiguan. The cardiac structural changes were observed by HE staining and electron microscopy. The expression of LC3II /I, Beclin1, m TOR, PKC Delta, PKC epsilon was detected by immunoblotting test (Western Blot), and the cardiomyocytes were detected by Tunel. Apoptosis. Results: 1. after a one-time high-intensity exercise, the structure and morphology of myocardial tissue and cells in the rat were changed to a certain extent. Compared with group EG, the myocardial fiber arrangement of the MEG group and AEG group, the morphology of mitochondria and the morphology of the nucleus were improved to a certain extent. The cell state of the group MEG was slightly better than that in the AEG group, suggesting the skin acupoint electricity. The effect of stimulation preconditioning on myocardial protection in rats was better than that of group.2.EG after percutaneous acupoint electrical stimulation. The apoptosis rate of group.2.EG was significantly higher than that in group E (P0.01). The apoptosis level of group MEG was significantly down, and the apoptosis of group AEG also decreased (P0.01), but the degree of downregulation was lower than that in MEG group.Tunel detection results. The apoptosis of cardiac myocytes was induced, and the percutaneous point electrical stimulation could reduce the apoptosis rate of myocardial tissue. The effect of percutaneous acupoint electrical stimulation was better than that of post processing.3. immunoblotting test (Western Blot). The ratio of LC3II/I in group EG was significantly higher than that in group E (P0.05), suggesting that high intensity exercise could activate autophagy and MEG group. It was higher than group EG (P0.05), suggesting that the preconditioning of percutaneous acupoint electrical stimulation could further induce autophagy and protect the heart. The expression level of AEG group was not significantly higher than that of the MEG group (P0.05). The expression of Beclin1 in cardiac myocytes was slightly lower than that of the E group, but the decrease was smaller, and the expression level of Beclin1 in MEG group was improved. P0.05, suggesting that the expression of Beclin1 can be up-regulated by preconditioning with acupoint electrical stimulation (P0.05), and the expression level of AEG group also rises, and has significant difference (P0.05), and the expression level of AEG and MEG groups is not very different, MEG group is slightly higher, but the gap is not obvious (P0.05), suggesting percutaneous acupoint electrical stimulation preconditioning. The expression of M TOR expression in cardiac myocytes was significantly lower than that in group E (P0.01), while the expression of MEG group and AEG group was significantly lower than that of the E group and EG group (P0.01), while the decline potential of the MEG group and AEG group was more significant than that in the group of MEG and AEG. The expression of the AEG group was more significant than that of the E group. The expression of the MEG TOR in the MEG group and AEG group was more significant than that in the group. The expression of PKC Delta in group EG was up-regulated in group E, and in group MEG and AEG was down downward trend (P0.05), suggesting that a large intensity exercise could increase the expression of PKC Delta, and the expression of MEG could be inhibited by skin acupoint electrical stimulation. The expression of PKC E in cardiac myocytes was not obvious, and there was no significant change in each group at the same level (P0.05). Conclusion: 1, 1 Secondary intensive exercise (6% weight negative) can activate autophagy and apoptosis of rat cardiac myocytes at the same time. The percutaneous acupoint electrical stimulation can protect myocardium by up regulation of autophagy and inhibition of apoptosis in cardiac myocytes. The effect of.2 on myocardial apoptosis rate is better than that of post-processing. The autophagy phase activated by both of them is better than that of postprocessing. There is no significant difference in.4, and the expression of PKC delta can be up-regulated by a one-time high-intensity exercise. The preconditioning and post-processing of percutaneous acupoint electrical stimulation can inhibit the overexpression of PKC Delta in rat cardiac myocytes and play the protective effect of.5. There is no significant effect on the expression of PKC e by short cycle one-time large intensity exercise and single transcutaneous electrical stimulation.

【學(xué)位授予單位】：武漢體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 徐曉;方劍喬;張奕;劉侃卓;;經(jīng)皮穴位電刺激治療粘連前期肩周炎臨床觀察[J];上海針灸雜志;2006年07期

2 張嶸;;一種標(biāo)準(zhǔn)化的經(jīng)皮穴位電刺激方法用于解除吸煙者的煙癮[J];生理科學(xué)進(jìn)展;2009年02期

3 盛明山;;經(jīng)皮穴位電刺激治療周圍性面神經(jīng)麻痹[J];上海針灸雜志;1996年S1期

4 胡興國;經(jīng)皮穴位電刺激強(qiáng)度對(duì)術(shù)后鎮(zhèn)痛需求的影響[J];國外醫(yī)學(xué).麻醉學(xué)與復(fù)蘇分冊;1998年06期

5 米慧;鞏愛玲;孫偉;馮雪花;李玉;;經(jīng)皮穴位電刺激治療卵巢低反應(yīng)30例療效觀察[J];山東中醫(yī)藥大學(xué)學(xué)報(bào);2013年06期

6 劉侃卓;;經(jīng)皮穴位電刺激治療粘連前期和粘連期肩周炎89例[J];上海針灸雜志;2007年05期

7 葛建強(qiáng);張宏;;經(jīng)皮穴位電刺激聯(lián)合提肛運(yùn)動(dòng)治療慢性Ⅲ型前列腺炎60例[J];陜西中醫(yī);2009年01期

8 朱丹華;童基均;潘敏;陳裕泉;陳華德;;經(jīng)皮穴位電刺激鎮(zhèn)痛的腦電研究[J];浙江大學(xué)學(xué)報(bào)(工學(xué)版);2009年12期

9 張磊;羅芳;李歡冬;王保國;劉曉媛;;丘腦在經(jīng)皮穴位電刺激鎮(zhèn)痛效果中的作用[J];中國康復(fù)理論與實(shí)踐;2011年11期

10 鄧林;楊小成;鄧田;湯偉;陳訪;;經(jīng)皮穴位電刺激對(duì)婦科腹腔鏡術(shù)后惡心嘔吐的影響[J];中國中醫(yī)急癥;2013年06期

相關(guān)會(huì)議論文 前10條

1 王均爐;任秋生;沈財(cái)成;謝文霞;鄭瑞獻(xiàn);倪劍武;;經(jīng)皮穴位電刺激對(duì)開顱術(shù)圍術(shù)期腦損傷相關(guān)因子的影響[A];2007年浙江省麻醉學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2007年

2 王均爐;任秋生;沈財(cái)成;謝文霞;鄭瑞獻(xiàn);倪劍武;;經(jīng)皮穴位電刺激對(duì)開顱術(shù)圍術(shù)期腦損傷相關(guān)因子的影響[A];第九屆全國針刺麻醉針刺鎮(zhèn)痛及針刺調(diào)整效應(yīng)學(xué)術(shù)研討會(huì)論文集[C];2007年

3 唐巖峰;;經(jīng)皮穴位電刺激對(duì)婦科腹腔鏡術(shù)后惡心嘔吐的防治作用[A];2009年浙江省麻醉學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2009年

4 謝文霞;王均爐;趙紅琴;李利紅;吳晶;孟軼男;;經(jīng)皮穴位電刺激防治術(shù)后鎮(zhèn)痛尿潴留的療效評(píng)價(jià)[A];2011浙江省針灸學(xué)會(huì)年會(huì)暨學(xué)術(shù)交流會(huì)論文匯編[C];2011年

5 鄭娟麗;洪霞;沈鋒;崔q輝;;經(jīng)皮穴位電刺激對(duì)腰麻下痔瘺術(shù)后尿潴留的影響[A];2013年浙江省肛腸外科學(xué)術(shù)年會(huì)暨結(jié)直腸疾病的微創(chuàng)及綜合治療新進(jìn)展學(xué)習(xí)班論文匯編[C];2013年

6 王均爐;任秋生;;經(jīng)皮穴位電刺激對(duì)顱腦手術(shù)圍術(shù)期腦氧腦糖代謝的影響[A];2007年浙江省麻醉學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2007年

7 王均爐;任秋生;謝文霞;;經(jīng)皮穴位電刺激對(duì)顱腦手術(shù)圍術(shù)期腦氧腦糖代謝的影響[A];第九屆全國針刺麻醉針刺鎮(zhèn)痛及針刺調(diào)整效應(yīng)學(xué)術(shù)研討會(huì)論文集[C];2007年

8 吳鎏楨;閻俊娟;韓濟(jì)生;;2/100Hz經(jīng)皮穴位電刺激對(duì)27例青少年網(wǎng)絡(luò)成癮癥的治療作用[A];第九屆全國針刺麻醉針刺鎮(zhèn)痛及針刺調(diào)整效應(yīng)學(xué)術(shù)研討會(huì)論文集[C];2007年

9 陳明顯;葉開升;金曼;蔡淦;;經(jīng)皮穴位電刺激法治療腹瀉型腸易激綜合征的隨機(jī)對(duì)照臨床研究[A];第三屆江浙滬中西醫(yī)結(jié)合高峰論壇論文匯編[C];2011年

10 金曼;葉開升;陳明顯;胡萍;王芹;盧躍棣;葉育雙;;經(jīng)皮穴位電刺激法治療腹瀉型腸易激綜合征的生活質(zhì)量研究[A];第二十四屆全國中西醫(yī)結(jié)合消化系統(tǒng)疾病學(xué)術(shù)會(huì)議專題報(bào)告及論文集[C];2012年

相關(guān)重要報(bào)紙文章 前2條

1 郭永紅 石田寅夫;可推廣經(jīng)皮穴位電刺激治療DPN[N];中國醫(yī)藥報(bào);2005年

2 ;經(jīng)皮穴位電刺激治肩周炎[N];中國中醫(yī)藥報(bào);2009年

相關(guān)博士學(xué)位論文 前3條

1 谷秋寒;經(jīng)皮穴位電刺激防治剖宮產(chǎn)術(shù)椎管內(nèi)麻醉后低血壓作用：隨機(jī)、對(duì)照臨床研究[D];第四軍醫(yī)大學(xué);2015年

2 鄭穎;經(jīng)皮穴位電刺激對(duì)IVF中卵巢儲(chǔ)備下降患者卵巢功能的影響[D];山東大學(xué);2016年

3 張玲;視網(wǎng)膜脫離患者生存質(zhì)量及經(jīng)皮穴位電刺激輔助手術(shù)鎮(zhèn)痛的臨床研究[D];成都中醫(yī)藥大學(xué);2010年

相關(guān)碩士學(xué)位論文 前10條

1 趙秋燕;術(shù)前經(jīng)皮穴位電刺激改善婦科腹腔鏡手術(shù)患者術(shù)后恢復(fù)質(zhì)量[D];福建醫(yī)科大學(xué);2015年

2 高輝;經(jīng)皮電刺激足三里穴在有機(jī)磷農(nóng)藥中毒患者搶救中的作用及安全性研究[D];河北醫(yī)科大學(xué);2015年

3 于晗;經(jīng)皮穴位電刺激與針刺療法治療功能性消化不良的臨床比較觀察[D];南京中醫(yī)藥大學(xué);2015年

4 朱同常;經(jīng)皮穴位電刺激治療慢性非細(xì)菌性前列腺炎合并精液不液化療效觀察[D];安徽中醫(yī)藥大學(xué);2015年

5 范圣華;經(jīng)皮穴位電刺激對(duì)2型糖尿病糖代謝障礙作用的研究[D];中國中醫(yī)科學(xué)院;2015年

6 胡先華;經(jīng)皮穴位電刺激輔助全身麻醉減少鎮(zhèn)痛藥及其副作用的隨機(jī)對(duì)照研究[D];第四軍醫(yī)大學(xué);2015年

7 郭鍵;經(jīng)皮穴位電刺激對(duì)胃癌患者術(shù)后全身炎性反應(yīng)綜合征發(fā)生率的影響[D];第四軍醫(yī)大學(xué);2015年

8 馬迎存;經(jīng)皮穴位電刺激治療帶狀皰疹后神經(jīng)痛的頻率特異性研究[D];北京中醫(yī)藥大學(xué);2016年

9 李朋安;經(jīng)皮穴位電刺激在腦癱患者SPR術(shù)后康復(fù)中的療效觀察[D];北京中醫(yī)藥大學(xué);2016年

10 丁艷;經(jīng)皮穴位電刺激聯(lián)合耳穴貼壓治療心腎不交型失眠的療效觀察[D];浙江中醫(yī)藥大學(xué);2016年

，

本文編號(hào)：1838882