本文選題：絕經(jīng)后骨質(zhì)疏松癥 + 電針　； 參考：《湖南中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:通過觀察電針對去卵巢雌性SD大鼠骨密度及下丘腦中瘦素與瘦素受體含量的影響,探討針刺治療絕經(jīng)后骨質(zhì)疏松癥的可能機(jī)制,為針刺治療絕經(jīng)后骨質(zhì)疏松癥的臨床應(yīng)用提供實驗依據(jù)。方法:將40只3月齡健康雌性SD大鼠隨機(jī)分為4組:假手術(shù)組、模型組、藥物組、電針組,每組10只。其中假手術(shù)組去除卵巢周圍脂肪組織,其余三組去除卵巢,手術(shù)造模結(jié)束后將大鼠適應(yīng)性喂養(yǎng)5天,待情況穩(wěn)定后進(jìn)行干預(yù)。電針組予以電針腎俞、足三里、關(guān)元、三陰交四穴并灌服生理鹽水,藥物組給予阿侖膦酸鈉并行固定處理,假手術(shù)組與模型組行固定處理并同時灌服生理鹽水,4組SD大鼠均每天干預(yù)1次,10次1個療程,共治療9個療程。療程結(jié)束后剔除大鼠右側(cè)股骨并分離出下丘腦,采用雙能X線骨密度儀檢測股骨骨密度,RT-PCR法測定下丘腦中LP m RNA、OB-R m RNA的定量。結(jié)果:1.大鼠行卵巢摘除后骨密度開始降低,療程結(jié)束后發(fā)現(xiàn)模型組、藥物組、電針組骨密度明顯低于假手術(shù)組(P0.05),但電針組、藥物組骨密度值高于模型組(P0.05)。2.模型組下丘腦中LP及OB-R的含量高于假手術(shù)組(P0.05),電針組和藥物組下丘腦中LP及OB-R的含量低于模型組(P0.05)。結(jié)論:1.電針腎俞、足三里、關(guān)元、三陰交能夠減少雌性SD大鼠去卵巢后骨量丟失,能夠防治絕經(jīng)后骨質(zhì)疏松癥。2.電針腎俞、足三里、關(guān)元、三陰交能夠抑制去卵巢后大鼠下丘腦中LP及OB-R含量的升高,削弱其骨形成中樞抑制作用,此調(diào)節(jié)方式可能具有減少絕經(jīng)后骨量丟失的作用。
[Abstract]:Objective: to investigate the possible mechanism of acupuncture in treating postmenopausal osteoporosis by observing the effects of electroacupuncture on bone mineral density (BMD) and the contents of leptin and leptin receptor in hypothalamus of ovariectomized female SD rats. To provide experimental evidence for the clinical application of acupuncture in the treatment of postmenopausal osteoporosis. Methods: forty healthy female SD rats aged 3 months were randomly divided into 4 groups: sham operation group, model group, drug group, electroacupuncture group, 10 rats in each group. The sham-operation group removed the periovarian adipose tissue and the other three groups removed the ovary. The rats were fed adaptively for 5 days after the operation. The electroacupuncture group was given electroacupuncture of Shenshu, Zusanli, Guan Yuan, Sanyinjiao four points and normal saline, while the drug group was treated with alendronate and fixed simultaneously. The sham operation group and the model group were treated with fixed treatment and were treated with saline at the same time. The SD rats in the 4 groups were treated with 10 times a course of treatment once a day for 9 courses of treatment. After the course of treatment, the right femur was removed and the hypothalamus was isolated. The bone mineral density (BMD) of femur was measured by dual-energy X-ray absorptiometry. The OB-R m RNA in the hypothalamus was measured by RT-PCR. The result is 1: 1. The BMD of the model group, drug group and electroacupuncture group was significantly lower than that of the sham operation group, but the BMD in the electroacupuncture group was higher than that in the model group. The content of LP and OB-R in hypothalamus of model group was higher than that of sham operation group, and the content of LP and OB-R in hypothalamus of electroacupuncture group and drug group was lower than that of model group. Conclusion 1. Electroacupuncture of Shenshu, Zusanli, Guan Yuan and Sanyinjiao can reduce bone loss after ovariectomy in female SD rats, and can prevent and treat postmenopausal osteoporosis. Electroacupuncture of Shenshu, Zusanli, Guan Yuan and Sanyinjiao could inhibit the increase of LP and OB-R content in hypothalamus of ovariectomized rats, and weaken the central inhibition of bone formation.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245

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