發(fā)布時(shí)間：2018-04-15 22:34

  本文選題：瘢痕灸預(yù)處理 + 肝俞��； 參考：《中華中醫(yī)藥學(xué)刊》2017年05期

【摘要】：目的:探討瘢痕灸預(yù)處理肝俞及足三里穴對(duì)原發(fā)性肝癌大鼠Wnt信號(hào)傳導(dǎo)通路相關(guān)蛋白β-連環(huán)蛋白(β-catenin)、分泌型卷曲相關(guān)蛋白(s FRP1)表達(dá)的影響。方法:48只Wistar雄性大鼠,隨機(jī)分為對(duì)照組、模型組、預(yù)處理肝俞組、預(yù)處理足三里組,每組12只。預(yù)處理肝俞組和預(yù)處理足三里組均提前2周施行瘢痕灸后,與模型組一同采用腹腔注射二乙基亞硝胺(50 mg/kg)建立大鼠原發(fā)性肝癌(primary liver cancer,PLC)模型,記錄各組大鼠體質(zhì)量,造模結(jié)束后取大鼠肝臟,采用Western blot方法測(cè)定β-catenin,s FRP1表達(dá)量,HE染色觀察大鼠肝臟組織形態(tài)學(xué)變化。結(jié)果:與對(duì)照組相比,模型組、預(yù)處理肝俞組和預(yù)處理足三里組的s FRP1表達(dá)量明顯下降(P0.05),β-catenin的表達(dá)量明顯升高(P0.05);與模型組比較,預(yù)處理肝俞組和預(yù)處理足三里組s FRP1表達(dá)量明顯升高(P0.05),β-catenin的表達(dá)量顯著降低(P0.05);與預(yù)處理肝俞組比較,預(yù)處理足三里組s FRP1表達(dá)量明顯降低(P0.05),β-catenin的表達(dá)量升高(P0.05)。結(jié)論:瘢痕灸預(yù)處理肝俞和足三里對(duì)預(yù)防原發(fā)性肝癌均有良性作用,且肝俞穴效果優(yōu)于足三里穴。
[Abstract]:Objective: to investigate the effect of scar moxibustion preconditioning at Ganshu and Zusanli points on the expression of 尾 -cateninine (尾 -cateninine), a secretory curl associated protein (FRP1), in rats with primary liver cancer (HCC).Methods Forty-eight male Wistar rats were randomly divided into control group, model group, Ganshu preconditioning group and Zusanli preconditioning group with 12 rats in each group.After preconditioning Ganshu group and Zusanli group were given scar moxibustion two weeks in advance, the primary liver cancer model was established by intraperitoneal injection of diethylnitrosamine 50 mg / kg together with the model group, and the body weight of each group was recorded.At the end of the model, the liver of rats was taken out. The expression of 尾 -cateninins FRP1 was determined by Western blot method and the histomorphology of the liver was observed by HE staining.Results: compared with the control group, the expression of s FRP1 and 尾 -catenin in the model group, Ganshu preconditioning group and Zusanli preconditioning group were significantly decreased, and the expression of 尾 -catenin was significantly increased in the model group, and compared with the model group, the expression of 尾 -catenin in the model group was significantly higher than that in the model group.The expression of s FRP1 and 尾 -catenin were significantly increased in Ganshu preconditioning group and Zusanli group, and the expression of 尾 -catenin was significantly decreased compared with that in Ganshu preconditioning group, the expression of s FRP1 in Zusanli preconditioning group was significantly lower than that in Ganshu preconditioning group, and the expression of 尾 -catenin was significantly increased in Zusanli preconditioning group.Conclusion: both Ganshu and Zusanli pretreated with scar moxibustion have benign effects on the prevention of primary liver cancer, and the effect of Ganshu point is better than that of Zusanli point.
【作者單位】： 遼寧中醫(yī)藥大學(xué)針灸推拿學(xué)院;沈陽(yáng)市中醫(yī)院內(nèi)科;遼寧中醫(yī)藥大學(xué)第一臨床學(xué)院;
【基金】：遼寧省科學(xué)技術(shù)基金項(xiàng)目(2014020047)
【分類號(hào)】：R245

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