本文選題：針刺　切入點：自發(fā)性高血壓大鼠　出處：《浙江中醫(yī)藥大學》2017年碩士論文

【摘要】：目的以自發(fā)性高血壓大鼠(SHR)為研究載體,觀察以"太沖"為主穴的不同經穴配伍(原絡配穴、同名經配穴、辨證配穴)對SHR血壓的影響,確定降壓效應最佳配穴組方;觀察針刺對SHR延髓頭端腹外側區(qū)(RVLM)天冬氨酸(AsP)、谷氨酸(Glu)的影響,初步探討針刺降壓的部分中樞作用機制。方法SHR 72只,隨機分為6組:模型組、太沖配內關組、太沖配蠡溝組、太沖配光明組、太沖配太溪組、太沖配非穴組,每組12只,另設同周齡WKY大鼠12只作為正常對照組。各治療組針刺相應雙側穴位,每次30min,每天1次,共15次;模型組及WKY組不予針刺。分別于治療第1、3、7、15天針刺結束后測量各組大鼠尾動脈血壓。模型組、太沖配內關組、太沖配非穴組于第15天測完血壓后立即處死,正常對照組大鼠同一天處死,用高效液相熒光法(HPLD-FLD)測定各組大鼠RVLM中Asp、Glu含量。結果一、大鼠血壓變化:1.收縮壓(SBP):針刺7天、15天后,與模型組比較,太沖配內關組、太沖配光明組、太沖配太溪組、太沖配非穴組SBP均明顯下降,差異有統(tǒng)計學意義(P0.05);針刺15天后,太沖配蠡溝組SBP降壓百分比大于模型組(P0.05),太沖配內關組SBP降壓百分比大于太沖配蠡溝組、太沖配光明組、太沖配太溪組、太沖配非穴組(P0.05);與針刺前比較,太沖配內關組在針刺15天后SBP、舒張壓(DBP)、平均動脈壓(MBP)均明顯下降,差異有統(tǒng)計學意義(P0.05);2.DBP:針刺7天、15天后,太沖配內關組DBP降壓百分比大于模型組,差異有統(tǒng)計學意義(P0.05);3.MBP:針刺7天、15天后,太沖配內關組MBP降壓百分比大于模型組,差異有統(tǒng)計學意義(P0.05);二、大鼠RVLM中Asp、Glu含量差異:與正常對照組比較,模型組Asp、Glu含量明顯升高,差異有統(tǒng)計學意義(P0.05);太沖配內關組Asp、Glu含量明顯低于模型組,差異有統(tǒng)計學意義(P0.05);太沖配內關組Asp含量明顯低于太沖配非穴組,差異有統(tǒng)計學意義(P0.05),太沖配內關組Glu含量較太沖配非穴組低,但差異無統(tǒng)計學意義(P0.05)。結論針刺具有降壓效應,以"太沖"為主穴的原絡配穴、同名經配穴、辨證配穴,對SHR的血壓均有調控作用,其中"太沖"配"內關"穴(同名經配穴)針刺降壓效應最佳;針刺降壓的中樞機制可能是通過影響大鼠RVLM中Asp、Glu實現(xiàn)的。
[Abstract]:Objective to observe the effect of different meridian combinations (original collaterals, syndromes, syndrome differentiation) on the blood pressure of SHR with "Taicong" as the main point. To observe the effect of acupuncture on aspartic acid aspartic acid (ASP) and glutamic acid (Glul) in the ventrolateral ventrolateral medulla of SHR, and to explore the mechanism of partial central effect of acupuncture on reducing blood pressure. Methods 72 rats of SHR were randomly divided into 6 groups: model group, Taichong plus Neiguan group, Taichong Baiguo group, Taichong Baiguo group. There were 12 rats in each group in Taichong combined with Taixi group, Taichong plus non-point group and 12 WKY rats of the same age as normal control group. Each treatment group was treated with acupuncture at corresponding bilateral acupoints for 30 minutes, once a day for 15 times. The blood pressure of caudal artery in each group was measured at the end of acupuncture at the end of 715 days after treatment. The model group, Taichong plus Neiguan group and Taichong plus non-point group were killed immediately after the blood pressure was measured on the 15th day. The rats in the control group were killed on the same day, and the content of Asp1 Glu in RVLM was determined by high performance liquid phase fluorescence method. Results 1. The blood pressure of rats changed to 1. Systolic blood pressure (SBP): after 7 days and 15 days acupuncture, compared with the model group, the rats were compared with Neiguan group. The SBP of Taichong group, Taichong and Taixi group, Taichong group and non-point group were significantly decreased (P 0.05), and the difference was significant after 15 days of acupuncture. The percentage of SBP hypotension in Taichong and Ligou group was higher than that in model group, and the percentage of SBP in Taichong and Neiguan group was higher than that in Taichong and Baiguang group, Taichong and Taixi group, Taichong and non-acupoint group, compared with that before acupuncture, the percentage of SBP in Taichong and Neiguan group was higher than that in Taichong Peiguang group, Taichong and Taixi group. After 15 days of acupuncture, SBP, DBP, and mean arterial pressure in Taichong and Neiguan group were significantly decreased, the difference was statistically significant (P 0.05) and 2.DBP: after 7 days and 15 days of acupuncture, the percentage of DBP in Taichong and Neiguan group was higher than that in model group. After 7 days and 15 days of acupuncture, the percentage of MBP in Taichong and Neiguan group was higher than that in model group, and the difference was statistically significant (P 0.05). Second, the content of AspP in RVLM in rats was significantly higher than that in normal control group. The content of Asp in Taichong Neiguan group was significantly lower than that in model group (P 0.05), and the content of Asp in Taichong injection Neiguan group was significantly lower than that in Taichong Neiguan group. The content of Glu in Taichong and Neiguan group was lower than that in Taichong and non-point group, but the difference was not statistically significant. Conclusion Acupuncture has the effect of lowering blood pressure. The blood pressure of SHR was regulated, among which "Taicong" combined with "Neiguan" point had the best effect of lowering blood pressure, and the central mechanism of acupuncture on blood pressure might be realized by affecting AspGlu-Glu in rat RVLM.
【學位授予單位】：浙江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245

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