本文關(guān)鍵詞：山藥多糖的提取分離及活性初步研究，由筆耕文化傳播整理發(fā)布。

網(wǎng)友1318384917近日為您收集整理了關(guān)于山藥多糖的提取分離及活性初步研究的文檔，希望對(duì)您的工作和學(xué)習(xí)有所幫助。以下是文檔介紹：江蘇大學(xué)碩士學(xué)位論文山藥多糖的提取分離及活性初步研究姓名:高英春申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):生藥學(xué)指導(dǎo)教師:陳鈞20090604江蘇大學(xué)碩士學(xué)位論文摘要山藥是一種多年生纏繞草質(zhì)藤本植物,為薯蕷科薯蕷屬植物薯蕷(DioscoreaoppositeThunb.)的塊莖,具有健脾、補(bǔ)肺、固腎等功效。多糖是山藥中的主要活性成分,具有增強(qiáng)免疫功能,降血糖、降血脂、抗氧化、抗衰老等多種生理活性。本課題以江蘇東臺(tái)產(chǎn)山藥為研究對(duì)象,對(duì)其中的多糖類成分進(jìn)行了研究,為開發(fā)利用山藥資源,提供理論依據(jù)。本論文通過單因素和正交試驗(yàn)優(yōu)化了山藥片粗多糖的提取條件,確定了山藥片粗多糖的實(shí)驗(yàn)室最佳提取條件為:提取溫度100*C,提取時(shí)間為3h,料液比為1:12,pH為8,提取液真空濃縮至原體積的1/5,所用95%乙醇的體積為多糖濃縮液體積的3倍。采用該工藝提取的山藥粗多糖,粗多糖得率為11.58%,苯酚一硫酸法測(cè)定山藥多糖含量為67.03%。以蛋白脫除率和多糖保留率為指標(biāo),對(duì)山藥粗多糖中蛋白質(zhì)脫除方法(鹽酸法、三***乙酸法、胰蛋白酶法)進(jìn)行了比較,確定最佳方法為胰蛋白酶法,最佳條件為:濃度lmg/ml山藥粗多糖溶液10ml,酶用量0.5ml,酶解時(shí)間45 min,酶解溫度37℃。在此條件下,蛋白脫除率為88.7%,多糖保留率為93.1%。山藥多糖經(jīng)除蛋白、除色素、流水透析后經(jīng)DEAE--52纖維素層析柱,得到RDPd.I、RDPd.II兩種組分,再分別過Sephadex G.100凝膠柱進(jìn)一步純化,得到RDPs.I、RDPs.II兩組分,經(jīng)高效液相檢測(cè),呈單一對(duì)稱峰,表明達(dá)到一定的均一純度。采用UV、IR、HPLC等現(xiàn)代分析手段,分析了山藥多糖的兩種組分RDPs.I、RDPs.II。紫外光譜在260nm一600nm處無明顯的吸收,表明經(jīng)分離純化的山藥多糖的兩種組分RDPs-I、RDPs—II均不含核酸、蛋白質(zhì),紅外光譜顯示有糖的特征吸收峰。RDPs.I、RDPs.II紅外圖譜中未見810cm。1和870cm‘1處的吸收峰,可能均不含甘露糖。HPLC測(cè)定分子量結(jié)果為:RDPs.1分子量為7.62×103 Da,RDPs.11分子量為6.74x10a Da。論文對(duì)山藥粗多糖的藥理活性進(jìn)行了初步研究,山藥粗多糖對(duì)正常小鼠的體重、血糖值、肝糖原含量沒有影響。山藥粗多糖具有降低糖尿病小鼠血糖、血脂的作用,能夠提高糖尿病小鼠葡萄糖耐受能力,促進(jìn)肝糖原合成,增加肝糖原含量。體外抗腫瘤試驗(yàn)表明:山藥多糖純化后得到的RDPs.I、RDPs.II在濃度較低時(shí),對(duì)人肝癌細(xì)胞HepG2呈現(xiàn)促進(jìn)作用;RDPs—II對(duì)HepG2細(xì)胞有一定的抑制作用。江蘇大學(xué)碩士學(xué)位論文關(guān)鍵詞: 山藥多糖提取純化降血糖抗腫瘤Ⅱ江蘇大學(xué)碩士學(xué)位論文ABSTRACTRhmoma dioscoreae is one kind of herbaceous perennial vine,the stem belongs toDioscorea opposite Thunb.It is good for spleen,lung and kindey.harides is the mainactive ingredient in Rhizoma dioscoreae,which has the function of enhancing immunefunction,redudng blood sugar and blood fat,anti—oxidation;anti-aging and SO on.This articlechooses the Rhizoma dioscoreae produced in DongTai Jiang Su Pmvmce,and study harides in order to provide the theorical evidence to develop the source of Rhizomadioscoreae.The article studies the extraction technology of Rhmoma dioscoreae halides.Single factor test and orthogonal experiment design method L9(34)were applied.The optimalextraction technology of Rhizoma dioscoreae pieces is with water 12 times for 3H,100"C,pH=8,harides solution concentrate to 1/5,the volume of 95%ethanol is 3 times morethan harides concentrated liquid.The extraction rate of harides of Rhizomadioscoreae is 11.58%,and halide content is 67.03%.Three methods ale used to remove protein of Rhizoma dioscoreae harides.Theoptimal factor for removing protein is:RDCP solution 1 0ml(1mg/m1),TPS 0.5ml,posing time 45min and posing temperature 37"(2.The removing rate for protein is88.7%and retaining rate for harides is 93.1%.The Rh娩oma dioscoreae harides is done by dismissing protein and pigment,flow dialysis and DEAE一52 cellulose column chromatography,the Rhmoma harides Was separated 2 parts,RDPd·I and RDPd-II.And then they purified bySephadex G-100 column chromatography,RDPs-I and RDPs—II are received.They ale singlesymmetrical peaks detected by HPLC.It concluded that the ponents of Rhmomadioscoreae harides are pure.The article studies the RDPs—I and RDPs—II by uv,IR,HPLC and GC-MS.Theyshowed characteristic absorption peaks on UV and IR.It cannot see

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  本文關(guān)鍵詞：山藥多糖的提取分離及活性初步研究，由筆耕文化傳播整理發(fā)布。