本文關(guān)鍵詞： 調(diào)神健脾針刺法 促腎上腺皮質(zhì)激素釋放激素2型受體 基因多態(tài)性 有效組 無效組　出處：《南京中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:研究促腎上腺皮質(zhì)激素釋放激素2型受體相關(guān)單核苷酸序列的多態(tài)性與腸易激綜合征調(diào)神健脾針刺法療效的相關(guān)性。方法:將隨機(jī)分入針刺組的116例患者進(jìn)行為期6周的調(diào)神健脾針刺法治療,第0周和第6周填寫IBS-SSS量表作為癥狀等級(jí)評(píng)價(jià)的依據(jù),歸類分組為有效組和無效組。所有患者的血樣經(jīng)過QIAGEN法提取DNA進(jìn)行DNA以及CRHR2相關(guān)單核苷酸鏈基因多態(tài)性的分析。通過互聯(lián)網(wǎng)上搜索國(guó)際SNP數(shù)據(jù)庫,檢索出在中國(guó)人群中rs2284217、rs2284220和rs3779250中以A/G為表現(xiàn)型,rs2240403、rs2267710以及rs4722999中以C/T為表現(xiàn)型。篩選具有統(tǒng)計(jì)學(xué)差異的6條單核苷酸鏈rs4722999、rs3779250、rs2240403、rs2267710、rs2284217和rs2284220作為基因多態(tài)性分析對(duì)象。統(tǒng)計(jì)分析方法中DNA分析采用哈迪-溫伯格平衡檢驗(yàn),CRHR2基因多態(tài)性與調(diào)神健脾針刺法療效相關(guān)性采取3×2表卡方檢驗(yàn)和四格表檢驗(yàn)。結(jié)果:1.基線期有效組與無效組患者在年齡、體重、病程及IBS-SSS基線期分?jǐn)?shù)上無統(tǒng)計(jì)學(xué)差異(P0.05),哈迪-溫伯格檢驗(yàn)結(jié)果示全部P0.05,說明群體基因遺傳平衡,數(shù)據(jù)來自同一蒙德爾群體。2.rs2284217、rs2284220基因型AG與調(diào)神健脾針刺法療效具有相關(guān)性,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。3.單核苷酸鏈rs2284217、rs2284220、rs3779250中的基因型AG與調(diào)神健脾針刺法療效具有相關(guān)性,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:腸易激綜合征患者rs2284217、rs2284220、rs3779250上攜帶的基因型AG與調(diào)神健脾針刺法療效具有相關(guān)性,采用調(diào)神健脾針刺法治療攜帶該類基因分型患者的療效明顯優(yōu)于其他CRHR2基因亞型患者。
[Abstract]:Objective: to study the relationship between the polymorphism of single nucleotide sequence associated with adrenocorticotropin releasing hormone type 2 receptor and the therapeutic effect of acupuncture of regulating spirit and strengthening spleen in irritable bowel syndrome. 116 patients in acupuncture group were randomly divided into acupuncture group and treated with acupuncture therapy of regulating spirit and strengthening spleen for 6 weeks. At week 0 and week 6, the IBS-SSS scale was filled out as the basis for the evaluation of symptom grade. All patients' blood samples were extracted from DNA by QIAGEN method for the analysis of DNA and CRHR2 related single nucleotide chain gene polymorphism. International SNP database. Rs2284217rs2284220 in Chinese population and rs2240403 in rs3779250 with A / G as phenotype were obtained. Using C / T as phenotype in rs2267710 and rs4722999, six single nucleotide chains rs4722999 and rs3779250 with statistical difference were screened. Rs2240403,rs2267710. Rs2284217 and rs2284220 were used as gene polymorphism analysis objects. Hardy Weinberg equilibrium test was used for DNA analysis in statistical analysis method. The correlation between the polymorphism of CRHR2 gene and the therapeutic effect of acupuncture of regulating spirit and strengthening spleen was determined by using 3 脳 2 table chi-square test and four-grid test. Results: 1. The age and weight of the patients in the baseline effective group and the ineffective group were in age and weight. There was no statistical difference in the course of disease and the score of IBS-SSS baseline period. The results of Hardy Weinberg test showed that all of them were P0.05, indicating the genetic balance of the population. Data from the same Mondel population. 2.rs2284217rs2284220 genotype AG was correlated with the therapeutic effect of acupuncture for regulating spirit and invigorating spleen. The difference was statistically significant (P 0.05). Single nucleotide chain rs2284217rs2284220. The genotype AG in rs3779250 was correlated with the acupuncture effect of regulating spirit and invigorating spleen, and the difference was statistically significant (P 0.05). Conclusion: the patients with irritable bowel syndrome have rs2284217. The genotype AG carried on rs2284220rs3779250 was correlated with the therapeutic effect of acupuncture of regulating spirit and strengthening spleen. The therapeutic effect of Tiaoshen Jianpi acupuncture was better than that of other subtypes of CRHR2 gene in the treatment of patients with this kind of genotyping.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R246.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 朱佳杰;劉珊;蘇曉蘭;郭宇;李依潔;宋熠林;魏瑋;;CRF1和CRF2受體在腸易激綜合征內(nèi)臟高敏感中的作用[J];中國(guó)中西醫(yī)結(jié)合消化雜志;2017年01期

2 張玉;張軍峰;楊亞平;;近十五年來腸道微生態(tài)的中醫(yī)藥研究概況[J];遼寧中醫(yī)雜志;2016年11期

3 馬艷苗;梁琦;王世民;周然;柴智;;整合醫(yī)學(xué)視野下的腦腸新論[J];中醫(yī)雜志;2016年18期

4 徐萬里;孫建華;;針灸治療腸易激綜合征的研究進(jìn)展[J];世界中醫(yī)藥;2016年06期

5 張道偉;禚昌紅;劉維明;;腸易激綜合征中醫(yī)研究進(jìn)展[J];實(shí)用中醫(yī)藥雜志;2016年08期

6 張引強(qiáng);唐旭東;郭朋;王鳳云;卞立群;;肝胃相關(guān)理論在功能性胃腸病中的應(yīng)用[J];新中醫(yī);2016年08期

7 陸夏敏;陸敏;;從腦-腸軸角度探討腸易激綜合征的中醫(yī)治療[J];河南中醫(yī);2016年06期

8 王建斌;續(xù)海嘯;;中醫(yī)藥物治療腹瀉型腸易激綜合征的臨床研究進(jìn)展[J];光明中醫(yī);2016年04期

9 劉添文;李倩;楊小波;;基于文獻(xiàn)研究的腸易激綜合征中醫(yī)辨治思路探析[J];新中醫(yī);2016年02期

10 張科;曹蕊蕓;諸孟娟;李方平;牟小東;殷唯棟;黃君蘭;周潔;;腹瀉型腸易激綜合征中醫(yī)證型與焦慮、抑郁狀態(tài)的相關(guān)性研究[J];遼寧中醫(yī)雜志;2016年01期

相關(guān)會(huì)議論文 前1條

1 林穗玲;鐘穎然;陳曉敏;周福生;;CRH通路與D-IBS的相關(guān)性研究[A];中華中醫(yī)藥學(xué)會(huì)脾胃病分會(huì)第二十四次全國(guó)脾胃病學(xué)術(shù)交流會(huì)論文匯編[C];2012年

相關(guān)碩士學(xué)位論文 前3條

1 李霞;促腎上腺皮質(zhì)激素釋放激素對(duì)腸道屏障的影響研究[D];山東大學(xué);2016年

2 林穗玲;基于心胃相關(guān)理論探討中藥康泰復(fù)方對(duì)D-IBS CRH及CRH-R1的影響[D];廣州中醫(yī)藥大學(xué);2011年

3 倪瑾;腸易激綜合征羅馬Ⅲ與羅馬Ⅱ診斷標(biāo)準(zhǔn)的比較[D];山東大學(xué);2007年

，

本文編號(hào)：1455658