本文關(guān)鍵詞：“肺腸合治法”調(diào)控神經(jīng)肽VIP治療干燥綜合征的實(shí)驗(yàn)研究　出處：《南京中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 干燥綜合征 肺腸合治法 麥冬地芍湯 血管活性腸肽

【摘要】：目的：探討“肺腸合治法”治療干燥綜合征的中醫(yī)理論基礎(chǔ)；探討肺腸合治中藥麥冬地芍湯治療干燥綜合征調(diào)節(jié)Th1/Th2、Th17/Treg免疫失衡及AQP5表達(dá)異常是否與其上調(diào)神經(jīng)肽VIP的表達(dá)相關(guān)。方法：33只雌性NOD小鼠隨機(jī)分為3組：模型組、羥氯喹組、中藥組,8只ICR (Institute of Cancer Research)小鼠為空白組。羥氯喹組、中藥組分別灌服羥氯喹溶液及麥冬地芍湯,空白及模型組灌服生理鹽水,18周齡時(shí)采集頜下腺及血清。檢測(cè)小鼠10、13、16周齡時(shí)的唾液流率,Elisa法檢測(cè)血清及一側(cè)頜下腺中VIP及細(xì)胞因子IFN-γ、IL-10、IL-17A的含量,另一側(cè)頜下腺作HE染色,免疫組化法測(cè)AQP5的表達(dá)。結(jié)果：1.與空白組相比,模型組小鼠唾液流率明顯減少,與模型組相比,中藥組小鼠在16周齡時(shí)唾液流率明顯改善(P0.01)。2.中藥組小鼠血清及頜下腺中VIP濃度較模型組明顯升高(P0.05),IFN-γ、IL-17A水平較模型組降低(P0.01),Thl/Th2、Th17/Treg比值較模型組明顯降低(P0.01)。3.頜下腺病理顯示,NOD小鼠較正常小鼠頜下腺可見(jiàn)明顯淋巴細(xì)胞浸潤(rùn),而經(jīng)麥冬地芍湯治療,頜下腺病理評(píng)分明顯改善(P0.05)。免疫組化顯示模型組小鼠AQP5在頜下腺的表達(dá)明顯減低,羥氯喹及中藥組AQP5的表達(dá)較模型組上調(diào)(P0.05)。結(jié)論：麥冬地芍湯對(duì)干燥綜合征動(dòng)物模型NOD小鼠有顯著療效,改善了小鼠唾液流率,調(diào)節(jié)了Thl/Th2、Th17/Treg免疫平衡,改善頜下腺淋巴細(xì)胞浸潤(rùn),將AQP5的表達(dá)上調(diào),其機(jī)制可能與麥冬地芍湯上調(diào)血清及頜下腺中VIP的表達(dá)相關(guān)。
[Abstract]:Objective: to explore the theoretical basis of traditional Chinese medicine for the treatment of Sjogren's syndrome. Objective: to explore the treatment of Sjogren's syndrome (Sjogren's syndrome) with Radix Ophiopogonis decoction for regulating Th1/Th2. Th17/Treg immune imbalance and abnormal AQP5 expression were correlated with the up-regulation of neuropeptide VIP. Methods Thirty three female NOD mice were randomly divided into three groups: model group and hydroxychloroquine group. Eight ICR Institute of Cancer research mice in Chinese medicine group were divided into blank group and hydroxychloroquine group. The rats in the traditional Chinese medicine group were given hydroxychloroquine solution and Ophiopogonium root decoction respectively. The submandibular gland and serum were collected in the blank group and the model group at the age of 18 weeks. The saliva flow rate of the mice at the age of 101316 weeks was measured. The contents of VIP and cytokine IFN- 緯 IL-10 IL-17A in serum and one side of submandibular gland were detected by Elisa method, and HE staining was performed on the other side of submandibular gland. Results: 1. Compared with the blank group, the saliva flow rate of the model group was significantly lower than that of the model group. The saliva flow rate in the Chinese medicine group was significantly improved at the age of 16 weeks. The concentration of VIP in serum and submandibular gland of the traditional Chinese medicine group was significantly higher than that of the model group, and the level of IFN- 緯 in the serum and submandibular gland of the traditional Chinese medicine group was significantly higher than that of the model group. Compared with the model group, the level of IL-17A was significantly lower than that of the model group. The ratio of Thl / Th2T17 / Treg was significantly lower than that of the model group. The lymphocyte infiltration in submandibular gland of NOD mice was more obvious than that in normal mice, and was treated with Maidong Di Shao decoction. The pathological score of submandibular gland was improved significantly (P 0.05). The expression of AQP5 in the submandibular gland of the model group was significantly decreased by immunohistochemistry. The expression of AQP5 in hydroxychloroquine group and traditional Chinese medicine group was higher than that in model group P0.050.Conclusion: Meidongdi Shao decoction has significant curative effect on NOD mice model of Sjogren syndrome and improves saliva flow rate of mice. It regulated the immune balance of Thl / Th2 and Th17 / Treg, improved submandibular gland lymphocytic infiltration, and up-regulated the expression of AQP5. The mechanism may be related to the up-regulation of VIP expression in serum and submandibular gland by Maidong Dizhao decoction.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R259

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