發(fā)布時間：2018-01-14 09:09

  本文關(guān)鍵詞：定喘湯對呼吸道合胞病毒感染兒童及大鼠的臨床與基礎(chǔ)研究　出處：《大連醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 定喘湯 RSV 毛細(xì)支氣管炎 Th1/Th2 大鼠 免疫細(xì)胞因子

【摘要】：第一部分定喘湯對呼吸道合胞病毒誘導(dǎo)大鼠肺炎免疫細(xì)胞因子作用的研究目的:本研究通過建立呼吸道合胞病毒感染幼齡大鼠肺炎模型,運(yùn)用Elisa方法檢測分析RSV感染后各組大鼠血清中免疫細(xì)胞因子(IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17)的變化,從而反應(yīng)RSV感染后Th1/Th2相關(guān)細(xì)胞因子失衡的機(jī)制,進(jìn)一步研究定喘湯對RSV感染后大鼠體內(nèi)免疫細(xì)胞因子的影響,闡明定喘湯防治RSV感染的作用機(jī)制,為減少RSV感染后哮喘發(fā)病提供科學(xué)的依據(jù)。方法:1.將36只Wistar大鼠隨機(jī)分為對照組、RSV感染組、利巴韋林組、定喘湯組,每組各9只,除對照組外,余各組均用RSV(Long株)病毒予大鼠滴鼻,滴鼻后對照組和RSV感染組以等體積的生理鹽水灌胃,各干預(yù)組分別給于相應(yīng)的藥物灌胃。通過觀察各組大鼠的一般狀態(tài)、肺指數(shù)、病理改變情況等指標(biāo),來評估定喘湯對大鼠RSV肺炎的防治作用。2.運(yùn)用酶聯(lián)免疫吸附法(Elisa)對各組大鼠的血清免疫細(xì)胞因子IFN-γ、IL-4、IL-10、IFN-γ/IL-4、IL-13、IL-33、IL-17 水平進(jìn)行檢測。結(jié)果:1.RSV感染后第7天,與對照組大鼠相比,RSV感染組大鼠一般狀態(tài)及肺組織病理情況均較差,肺指數(shù)增加有顯著差異(P0.05);與RSV感染組大鼠比較,定喘湯組及利巴韋林組大鼠一般狀態(tài)及肺組織病理情況均得到改善,肺指數(shù)降低有顯著差異(P0.05);利巴韋林組大鼠與定喘湯組大鼠比較,無顯著差異(P0.05)。2.血清中各細(xì)胞因子表達(dá)結(jié)果顯示:與對照組比較,RSV感染組的IFN-γ和IFN-γ/IL-4降低及其余各項指標(biāo)均升高具有顯著統(tǒng)計學(xué)意義(P0.05);與RSV感染組比較,定喘湯組 IFN-γ 和 IFN-γ/IL-4 升高,IL-4、IL-10、IL-13、IL-33、IL-17均降低具有顯著統(tǒng)計學(xué)意義(P0.05);與利巴韋林組比較,定喘湯組IFN-γ升高,IL-33降低具有顯著統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.定喘湯能減輕RSV感染后大鼠肺部病理損傷,有效防止RSV感染。2.RSV感染大鼠后血清中Th1相關(guān)細(xì)胞因子下降,Th2相關(guān)細(xì)胞因子上升,機(jī)體出現(xiàn)Th1/Th2平衡漂移。3.定喘湯干預(yù)RSV感染的大鼠后可以明顯抑制Th2相關(guān)細(xì)胞因子反應(yīng),增強(qiáng)Th1相關(guān)細(xì)胞因子反應(yīng),對RSV感染后Th1/Th2細(xì)胞因子的失衡具有雙向調(diào)節(jié)作用。第二部分毛細(xì)支氣管炎住院患兒免疫細(xì)胞因子水平的研究目的:從Th1/Th2平衡角度探討毛細(xì)支氣管炎住院患兒血清中免疫細(xì)胞因子(IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17)水平的變化,來闡明毛細(xì)支氣管炎的發(fā)病機(jī)制,為預(yù)防其日后發(fā)展為哮喘提供理論依據(jù)。方法:1.采用前瞻性的研究方法,收集2016年9月到2017年2月于大連市兒童醫(yī)院呼吸科住院小于2歲毛細(xì)支氣管炎患兒為毛細(xì)支氣管炎組。同時選擇同期體檢健康同齡兒童為正常組,觀察兩組兒童免疫細(xì)胞因子的指標(biāo)變化。2.運(yùn)用酶聯(lián)免疫吸附法(Elisa)對正常組患兒及毛細(xì)支氣管炎組患兒血清IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17 水平進(jìn)行檢測。結(jié)果:血清中各細(xì)胞因子表達(dá)結(jié)果顯示:與對照組比較,毛細(xì)支氣管炎組的IL-4、IL-10、IL-13、IL-33、IL-17水平升高有顯著統(tǒng)計學(xué)意義(P0.05)。結(jié)論:小兒毛細(xì)支氣管炎發(fā)病與相關(guān)免疫細(xì)胞因子水平的變化密切相關(guān),機(jī)體處于Th1/Th2免疫失衡狀態(tài)。
[Abstract]:Objective to study the first part Dingchuan Decoction on rats induced by respiratory syncytial virus pneumonia immune cell factor: This study through the establishment of respiratory syncytial virus infection in young rat pneumonia model, using Elisa method to detect RSV infection after analysis of immune cells in serum factor (IFN- gamma, IL-4, IFN- y /IL-4, IL-10. IL-13, IL-33, IL-17) changes, and reaction mechanism of RSV infection after the imbalance of Th1/Th2 related cytokines, further study on the influence of Dingchuan Decoction on cytokines in rats after RSV infection, to clarify the mechanism of dingchuantang preventing RSV infection, to provide a scientific basis for reducing the incidence of asthma after RSV infection. Methods: 1. 36 Wistar rats were randomly divided into control group, RSV infection group, Leigh Bhave Lin group, dingchuantang group, 9 rats in each group, except the control group, the other groups were RSV (Long strain) virus to rat nasal drip after intranasal control Group and RSV infection group with equal volume of saline, the intervention group were given intragastric administration with corresponding drugs. The general state of rats were observed in the lung index, pathological changes and other indicators, to evaluate the Dingchuan Decoction on rat RSV pneumonia. The prevention and cure effect of.2. by enzyme-linked immunosorbent method (Elisa) serum cytokines IFN- in rats, IL-4, IL-10, IL-13, IFN- y /IL-4, IL-33, IL-17 levels were detected. Results: seventh days after 1.RSV infection, compared with control group, RSV infection and pathological condition of the general condition and lung tissue of rats were poor, lung there are significant differences in index increased (P0.05); and RSV infection group rats, dingchuantang group and ribavirin group rats general state and lung tissue pathological conditions were improved, the lung index decreased significantly (P0.05); compared with ribavirin group rats and dingchuantang group rats, no significant difference (P 0.05) the results showed that the expression of.2. in serum: compared with the control group, RSV infection group, IFN- gamma and IFN- gamma /IL-4 decreased and the rest indexes were increased significantly (P0.05); compared with RSV infection group, dingchuantang group IFN- IFN- and gamma gamma /IL-4 increased, IL-4, IL-10, IL-13, IL-33, IL-17 decreased significantly (P0.05); compared with ribavirin group, dingchuantang group IFN- y increased, there was a statistically significant decrease in IL-33 (P0.05). Conclusion: the lung injury rats reduced after RSV infection 1. dingchuantang, effectively prevent RSV infection in serum Th1 related cytokines decreased rats infected with.2.RSV, increase of Th2 cytokines, immune balance of Th1/Th2 drift.3. dingchuantang RSV infection in rats after intervention can significantly inhibit the reaction of Th2 related cytokines, enhance the reaction of Th1 related cytokines in RSV infected Th1/ The imbalance of Th2 cytokine has bidirectional regulation effect. The research purpose of the second part of bronchiolitis hospitalized children: the level of immune cytokine on immune cells in serum of children with bronchiolitis hospitalized in Th1/Th2 from the perspective of balance factor (IFN- gamma, IL-4, IL-10, IFN- y /IL-4, IL-13, IL-33, IL-17) level changes, to clarify the pathogenesis for the prevention of bronchiolitis, the day after the development to provide a theoretical basis for asthma. Methods: 1. using prospective research method, from September 2016 to February 2017 in the Department of respiration of Dalian city children's hospital less than 2 years old children with bronchiolitis as bronchiolitis group. At the same time choose the healthy children of the same age as normal group, observe two groups of children cytokines index changes of.2. by enzyme linked immunosorbent assay (Elisa) in normal children and children with IFN- bronchiolitis serum gamma, IL-4, IL-10, IFN- y /IL-4, IL-13, IL-33, IL-17 levels were detected. Results: the results showed that the expression of cytokines in serum: compared with the control group, bronchiolitis group IL-4, IL-10, IL-13, IL-33, IL-17 levels were statistically significant (P0.05). Conclusion: changes in children with bronchiolitis and the related immune cytokine level is closely related to the body in Th1/Th2 immune imbalance.

【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R272

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