本文關(guān)鍵詞：針刺曲池與合谷對(duì)發(fā)熱大鼠PGE2和cAMP及IL-1β含量的影響　出處：《湖南中醫(yī)藥大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的:通過針刺曲池、合谷穴觀察發(fā)熱大鼠體溫變化情況,檢測大鼠下丘腦組織中PGE2、c AMP及血漿IL-1β含量,探討針刺退熱效應(yīng)及其機(jī)理。方法:將大鼠隨機(jī)分成6組,除正常組外,將造模成功的50只大鼠按隨機(jī)表隨機(jī)分成模型組(灌服蒸餾水)、中藥組(灌服銀翹解毒顆�；鞈乙�)、西藥組(灌服阿司匹林混懸液)、單純針刺組(針刺曲池、合谷)、非經(jīng)非穴組(針刺曲池、合谷穴旁開0.5cm),每組10只。各組隨機(jī)抽取5只大鼠在治療后2h取腹主動(dòng)脈血及下丘腦,予以待檢血漿IL-1β及下丘腦組織中PGE2、c AMP的含量;各組其余的5只大鼠在治療后1h、2h、3h、4h分別測量體溫并記錄,并于治療后4h測量體溫后取腹主動(dòng)脈血及下丘腦,予以待檢相應(yīng)指標(biāo)。結(jié)果:1、對(duì)酵母致熱大鼠體溫的影響西藥組、中藥組、針刺組在治療后各個(gè)時(shí)間點(diǎn)體溫均下降,其中西藥組、針刺組在治療后各個(gè)時(shí)間點(diǎn)體溫均低于中藥組,針刺組治療后1h體溫低于西藥組,差異均有統(tǒng)計(jì)學(xué)意義(P0.01,P0.05);西藥組治療后2h,針刺組治療后4h體溫與正常組相當(dāng),差異無統(tǒng)計(jì)學(xué)意義(P0.05);非經(jīng)非穴組體溫變化與模型組相當(dāng),差異無統(tǒng)計(jì)學(xué)意義(P0.05)。2、對(duì)酵母所致發(fā)熱大鼠下丘腦組織中PGE2、c AMP及血漿IL-1β含量的影響各組治療2小時(shí)后,針刺組下丘腦組織中PGE2、c AMP、血漿IL-1β分別與模型組相比均有統(tǒng)計(jì)學(xué)意義(P0.05),與西藥組相比均有統(tǒng)計(jì)學(xué)意義(P0.05),西藥組與正常組相比無統(tǒng)計(jì)學(xué)意義(P0.05);治療4小時(shí)后,針刺組下丘腦組織中PGE2、c AMP、血漿IL-1β分別與模型組、中藥組相比均有統(tǒng)計(jì)學(xué)意義(P0.05),與西藥組、正常組相比均無統(tǒng)計(jì)學(xué)意義(P0.05)。給藥后2h、4h各組的體溫值分別與相應(yīng)PGE2、c AMP、IL-1β含量進(jìn)行相關(guān)分析,結(jié)果表明二者之間均存在明顯正相關(guān)(P0.01)。結(jié)論:1、針刺組、中藥組、西藥組均有退熱效應(yīng),其中西藥組、針刺組退熱效應(yīng)優(yōu)于中藥組,針刺退熱存在穴位特異性;針刺組退熱起效時(shí)間優(yōu)于西藥組,西藥組優(yōu)于中藥組;西藥組解熱時(shí)間優(yōu)于針刺組,針刺組優(yōu)于中藥組。2、針刺曲池、合谷退熱機(jī)制可能主要是通過降低大鼠下丘腦組織中PGE2、c AMP、血漿IL-1β含量而實(shí)現(xiàn)的。
[Abstract]:Objective: to observe the changes of body temperature in febrile rats by acupuncture of Quchi and Hegu points, and to detect the content of PGE2C AMP and plasma IL-1 尾 in hypothalamus of rats. Methods: the rats were randomly divided into 6 groups. In addition to the normal group, 50 successful rats were randomly divided into the model group (fed with distilled water) according to the random list. The traditional Chinese medicine group (Yinqiao jiedu granule suspension), the western medicine group (taking aspirin suspension, the simple acupuncture group) (acupuncture qu Chi, Hegu Wu, non-meridian non-point group). 10 rats in each group were randomly selected to collect abdominal aortic blood and hypothalamus 2 hours after treatment, and the plasma IL-1 尾 and the content of PGE2C AMP in hypothalamus were measured. The body temperature of the other 5 rats in each group was measured and recorded at 1h, 2h and 3h after treatment, and the abdominal aorta blood and hypothalamus were taken 4 hours after treatment. Results the body temperature of rats caused by yeast fever was decreased in the western medicine group, traditional Chinese medicine group and acupuncture group at each time point after treatment, among which the western medicine group. The body temperature of the acupuncture group was lower than that of the traditional Chinese medicine group at each time point after treatment, and the body temperature of the acupuncture group was lower than that of the western medicine group at 1 hour after treatment, and the difference was statistically significant. The body temperature in the western medicine group was similar to that in the normal group at 4 hours after treatment, and there was no significant difference between the two groups (P 0.05). The change of body temperature in non-meridian non-point group was similar to that in model group, but there was no significant difference (P 0.05). 2. PGE2 in hypothalamus of febrile rats induced by yeast was observed. The effect of c AMP and plasma IL-1 尾 on PGE2c AMP in hypothalamus of acupuncture group 2 hours after treatment. Compared with the model group, the plasma IL-1 尾 had statistical significance (P 0.05), and compared with the western medicine group, the plasma IL-1 尾 had statistical significance (P 0.05). There was no significant difference between the western medicine group and the normal group (P 0.05). After 4 hours of treatment, PGE2AMP and plasma IL-1 尾 in the hypothalamus of acupuncture group were significantly higher than those in model group and traditional Chinese medicine group. There was no significant difference between the normal group and the control group (P 0.05). The temperature of each group was correlated with the content of IL-1 尾 in the corresponding PGE2C AMPP 4 h after administration. The results showed that there was a significant positive correlation between the two groups (P 0.01). Conclusion the antipyretic effect of the western medicine group and the acupuncture group is better than that of the traditional Chinese medicine group, and the antipyretic effect of the western medicine group and the acupuncture group is better than that of the traditional Chinese medicine group. Acupuncture has acupoint specificity in antipyretic; The antipyretic effect of acupuncture group was better than that of western medicine group, and that of western medicine group was better than that of traditional Chinese medicine group. The antipyretic time of the western medicine group was better than that of the acupuncture group, and the acupuncture group was superior to the traditional Chinese medicine group. The content of plasma IL-1 尾 was achieved.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R245

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