本文關鍵詞：“百會”透“曲鬢”對急性期腦出血大鼠腦組織中Survivin表達影響的實驗研究　出處：《黑龍江中醫(yī)藥大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 百會透曲鬢針刺療法 急性期腦出血 細胞凋亡 Survivin

【摘要】：目的:采用"百會"透"曲鬢"穴的針刺方法,觀察在針刺治療過程中Survivin在腦出血后大鼠腦組織中的動態(tài)變化,觀察Survivin與腦出血后細胞凋亡之間的關系,從而探討其對急性期腦出血大鼠腦組織中Survivin表達的影響,為臨床上治療急性期腦出血提供有利的依據(jù)。方法:75只雄性Wistar大鼠,隨機分為空白組、模型組和針刺組。其中空白組15只大鼠,模型組和針刺組各30只,每組又平均分為6h,12h,24h,3d,7d五個時間點。其中針刺組和模型組采用自體血注入法建立腦出血模型。待大鼠蘇醒后觀察并記錄其神經(jīng)功能缺損程度,挑選符合條件的模型。對針刺組進行針刺"百會"透"曲鬢"的治療并對各組大鼠進行系統(tǒng)的實驗觀察:(1)觀察各組大鼠神經(jīng)功能情況;(2)采用免疫組化檢測各組大鼠腦組織中Survivin的相關動態(tài)表達;(3)采用TUNNEL法檢測各組大鼠腦出血后血腫周圍細胞凋亡情況。結(jié)果:(1)神經(jīng)功能評分結(jié)果:針刺組與模型組在12h、24h、3d的時間點比較有明顯差異,可觀察到針刺組神經(jīng)功能缺損評分得到明顯改善,有統(tǒng)計學意義(P0.01);(2)免疫組化結(jié)果顯示:通過針刺組和模型組比較,針刺組Survivin在各個時間點均有動態(tài)表達,尤其是在12h、24h、3d表達明顯增高(p0.01);(3)TUNEL法檢測結(jié)果:針刺組與模型組比較,針刺組在各個時間點細胞凋亡數(shù)量均在減少,尤其是在12h、24h、3d時間點差異有統(tǒng)計學意義。(P0.01)。結(jié)論:(1)針刺"百會"透"區(qū)鬢"穴針刺法對腦出血后神經(jīng)功能缺損有明顯改善,具有神經(jīng)保護作用;(2)針刺"百會"透"曲鬢"通過提高Survivin的表達,對腦出血后血腫周圍腦組織起到保護作用;(3)針刺"百會"透"曲鬢"能夠提高TUNEL的陽性表達,抑制細胞凋亡。
[Abstract]:Objective: using the "method of acupuncture of Baihui Qubin acupoint, observe the dynamic changes in the acupuncture treatment process of Survivin in rat brain after cerebral hemorrhage, the relationship between Survivin and apoptosis after intracerebral hemorrhage, and to explore its effect on the expression of Survivin in brain tissue of rats with acute cerebral bleeding, for the clinical treatment of acute cerebral hemorrhage with a favorable basis. Methods: 75 male Wistar rats were randomly divided into the blank group, the model group and the acupuncture group. There were 15 rats in the blank group, 30 in the model group and in the acupuncture group. Each group was divided into five time points of 6h, 12h, 24h, 3D and 7d. In the acupuncture group and the model group, the model of cerebral hemorrhage was established by injection of autologous blood. After the rats were awakened, the degree of nerve function defect was observed and recorded, and the model was selected. The acupuncture group acupuncture "Baihui" treatment through "Qubin" and the rats of each group were observed: (1) experimental system to observe the neural function of rats; (2) the relevant dynamic expression by using immunohistochemical Survivin was detected in the brain tissue of rats; (3) detected by TUNNEL the rats after ICH apoptosis. Results: (1) the nerve function score results: acupuncture group and model group in 12h, 24h, 3D time points, there was significant difference between the observed score, acupuncture group of neurological deficit improved significantly, with statistical significance (P0.01); (2) immunohistochemistry results showed that: the acupuncture group and model group comparison of expression of Survivin in the acupuncture group at each time point were dynamic, especially the expression is significantly increased in 12h, 24h, 3D (P0.01); (3) TUNEL test results: acupuncture group compared with model group, the acupuncture group at each time point the number of cell apoptosis was decreased, especially in 12h, were statistically significant 24h, 3D time points difference. (P0.01). Conclusion: (1) acupuncture "Baihui" through "temple area" point acupuncture can obviously improve the neurological function after cerebral hemorrhage, has a neuroprotective effect; (2) acupuncture "Baihui Qubin through improving the expression of Survivin, to protect the brain tissue around the hematoma after intracerebral hemorrhage; (3) acupuncture" Baihui Qubin can increase the expression of TUNEL, inhibit apoptosis.
【學位授予單位】：黑龍江中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245

【相似文獻】

相關期刊論文 前10條

1 李新毅,于為民,穆俊霞;急性期腦出血患者血尿素氮變化的觀察[J];中國中西醫(yī)結(jié)合腎病雜志;2001年09期

2 陳斌;;單唾液酸四己糖神經(jīng)節(jié)苷脂鈉治療急性期腦出血的療效觀察[J];實用心腦肺血管病雜志;2013年01期

3 孫永全;孫秀枝;李春;;單唾液酸四己糖神經(jīng)節(jié)苷脂治療急性期腦出血的療效分析[J];中國衛(wèi)生產(chǎn)業(yè);2013年25期

4 王淑華,韓金鳳,玉樹成,郭立梅,張麗,張桂英,黃艷平,董蘭;紫外線照射充氧自血回輸輔助治療急性期腦出血[J];齊齊哈爾醫(yī)學院學報;1995年01期

5 周士軍，梅存選;122例急性期腦出血病人異常心電圖分析[J];實用醫(yī)學雜志;1996年11期

6 萬曉強;鄭念東;張道寶;王山;衛(wèi)正洪;鄒雨芮;;單唾液酸四己糖神經(jīng)節(jié)苷脂鈉治療急性期腦出血的臨床分析[J];現(xiàn)代生物醫(yī)學進展;2013年32期

7 沈順姬,張淑琴,劉群,劉忠?guī)r;胰島素治療急性期腦出血的臨床應用[J];中風與神經(jīng)疾病雜志;2000年04期

8 侯麗英,楊麗麗;急性期腦出血患者血清及腦脊液甲狀腺激素的動態(tài)觀察[J];山西醫(yī)藥雜志;2001年01期

9 葉國強,楊學峰;中西醫(yī)結(jié)合治療急性期腦出血85例臨床觀察[J];河南外科學雜志;2005年04期

10 崔淑芹,王鳳頎;急性期腦出血病人的觀察及護理[J];醫(yī)學理論與實踐;2005年03期

相關會議論文 前4條

1 高旭光;王慕一;李新峰;李佳貴;;急性期腦出血的預后因素分析[A];中華醫(yī)學會全國第五次急診醫(yī)學學術(shù)會議論文集[C];1994年

2 單麗華;孫英健;孫明;;生大黃配合常規(guī)西藥治療急性期腦出血35例臨床觀察[A];第三屆全國中西醫(yī)結(jié)合神經(jīng)系統(tǒng)疾病學術(shù)會議論文集[C];2000年

3 燕明宗;李君梅;;丹參治療急性期腦出血療效觀察[A];山東省第三次中西醫(yī)結(jié)合神經(jīng)內(nèi)科學術(shù)研討會論文匯編[C];2011年

4 趙玉泊;張鵬程;;地壇牌清開靈治療急性期腦出血100例觀察[A];心腦病藥物臨床評價專家談[C];1998年

相關碩士學位論文 前1條

1 康曉禹;“百會”透“曲鬢”對急性期腦出血大鼠腦組織中Survivin表達影響的實驗研究[D];黑龍江中醫(yī)藥大學;2017年

，

本文編號：1342461