背景和目的:食管癌是常見(jiàn)的致死率較高的消化道惡性腫瘤之一,我國(guó)食管癌約90%屬于鱗狀細(xì)胞癌。Wnt信號(hào)通路在調(diào)控基因表達(dá)及細(xì)胞生物學(xué)行為中發(fā)揮重要作用。Wnt4屬于非經(jīng)典Wnt信號(hào)分子并且可調(diào)控上皮細(xì)胞分化,其表達(dá)異常與人類(lèi)鱗狀細(xì)胞癌具有相關(guān)性。JNK1作為非經(jīng)典wnt信號(hào)通路中位于細(xì)胞內(nèi)的重要信使分子,可介導(dǎo)腫瘤細(xì)胞的凋亡。本實(shí)驗(yàn)旨在研究非經(jīng)典Wnt信號(hào)通路中Wnt4、JNK1在食管鱗癌組織中的表達(dá)情況及其與臨床病理參數(shù)的關(guān)系,探討其在食管鱗癌發(fā)生發(fā)展中的意義。方法:收集、整理臨床資料及食管鱗癌、癌旁組織標(biāo)本;使用半定量PCR的方法檢測(cè)Wnt4、JNK1基因在食管鱗癌及癌旁組織中的m RNA表達(dá)情況;使用免疫組織化學(xué)染色方法檢測(cè)Wnt4及JNK1蛋白在食管鱗癌及癌旁組織中的表達(dá)情況;對(duì)Wnt4、JNK1表達(dá)水平綜合臨床病理資料進(jìn)行相關(guān)性研究,分析Wnt4/JNK1信號(hào)通路與食管鱗癌分化程度、病理分期、淋巴轉(zhuǎn)移、浸潤(rùn)深度之間的關(guān)系。結(jié)果:（1）食管鱗癌組織中Wnt4、JNK1 m RNA表達(dá)水平低于癌旁正常組織,差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。食管鱗癌高分化組中Wnt4 m ... 

【文章來(lái)源】：天津醫(yī)科大學(xué)天津市 211工程院校

【文章頁(yè)數(shù)】：76 頁(yè)

【學(xué)位級(jí)別】：碩士

【部分圖文】：

JNK1半定量PCR產(chǎn)物分子大小為355bp，凝膠電泳圖見(jiàn)下

其中M為

內(nèi)參（GAPDH）半定量PCR產(chǎn)物大小為156bp，凝膠電泳圖見(jiàn)下

【參考文獻(xiàn)】：
期刊論文
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