目的:探究趨化因子CXCL7作為結(jié)直腸癌（colorectal cancer,CRC）生物標志物的診斷價值;比較CRC患者癌組織和癌旁組織的表達差異,并研究趨化因子CXCL7對CRC細胞生物學(xué)功能的影響。方法:ELISA法定量測量280名CRC患者和280名正常人群血清CXCL7的濃度,比較兩組人群血清濃度的差異性,分析CRC患者血清CXCL7濃度與臨床資料的相關(guān)性;通過測試隊列、驗證隊列和交叉隊列分析CXCL7的診斷效能;建立Logistic多元回歸模型進行聯(lián)合診斷,計算聯(lián)合診斷因子,繪制ROC工作曲線,并計算AUC曲線下面積、靈敏度（Sensitivity）、特異度（Specificity）等指標;TNM、I-II、性別亞組分析進一步評價CXCL7的診斷效能;重新從不同醫(yī)院選擇CRC患者進行診斷試驗,進一步檢驗CXCL7作為生物標記物的穩(wěn)定性和外延推廣價值。使用免疫組化法檢測20名CRC患者癌組織和癌旁組織中CXCL7的表達情況,同時借助qPCR法測定患者癌組織和癌旁組織中CXCL7的表達水平,分析CXCL7在癌組織和癌旁組織的表達差異性。慢病毒轉(zhuǎn)染構(gòu)建LoVo過表達和Caco-2... 

【文章來源】：江南大學(xué)江蘇省 211工程院校 教育部直屬院校

【文章頁數(shù)】：52 頁

【學(xué)位級別】：碩士

【部分圖文】：

CRC流行病學(xué)“三間分布”數(shù)據(jù)

第二章血清趨化因子CXCL7在CRC臨床診斷中的價值探究13圖2-1CRC患者和對照組血清CXCL7和腫瘤相關(guān)抗原濃度散點圖Figure2-1.ScatterplotanalysisofserumconcentrationsofCXCL7andtumor-associatedantigensinpatientswithCRCandcontrols.(A)ComparisonofserumCXCL7concentrationsbetweenthecontrolandCRCgroups.(B)ComparisonofserumCXCL7concentrationsamongdifferentTNMsubgroups.(C–H)ComparisonsofserumCEA,CA125,andCA19-9levelsbetweenthecontrolandCRCgroupsandcomparisonsamongdifferentTNMsubgroups.TheMann-WhitneyU-testwasusedforcomparisonsbetweentwogroupsandtheKruskal-Wallistestwasappliedforanalysisofthreeormoregroups.*P<0.05,**P<0.01,***P<0.001,NS:Nostatisticalsignificance.表2-2CRC患者血清CXCL7與CRC患者臨床病理參數(shù)的相關(guān)性分析Table2-2.CorrelationbetweenCXCL7levelandclinicalcharacteristicsinpatientswithCRCVariableNumberMedianIQRrPvalueAge(years)<601031.781.46-2.160.0750.211≥601771.891.51-2.27SexMale1661.921.52-2.29-0.1430.016Female1141.761.46-2.15Tumersize<4cm1711.821.50-2.28-0.0370.542≥4cm1091.821.48-2.19HistologicalgradeWell141.691.48-2.13-0.0120.836Moderately1991.841.53-2.22

第二章血清趨化因子CXCL7在CRC臨床診斷中的價值探究1511514表2-4測試隊列與驗證隊列診斷試驗結(jié)果Table2-4.DiagnosticefficiencyofCXCL7determinedbyROCcurveanalysisintwosetsVariableMedianIQRCutoffAUC(95%CI)Sensitivity(%)Specificity(%)PvalueAsetAp1.841.51-2.211.430.872(0.826-0.910)81.9584.96<0.001Ac1.020.78-1.28BsetBp1.821.48-2.261.280.853(0.843-0.925)86.3980.95<0.001Bc1.010.82-1.23Cross-validationAp/Bc1.300.869(0.823-0.906)85.7181.63<0.001Bp/Ac1.300.859(0.812-0.897)84.3979.70<0.001圖2-2測試隊列與驗證隊列診斷試驗結(jié)果Figure2-2.CXCL7diagnostictestintrainingandvalidationsets.(A)AUCinthetrainingset.(B)SensitivityandspecificityinrelationtoserumCXCL7concentrationinthetrainingset.(C)Youdenindexinthetrainingset.(D)AUCinthevalidationset.(E)Sensitivityandspecificityinthevalidationset.(F)Youdenindexinthevalidationset.(G)AUCintheAp/Bcset.(H)SensitivityandspecificityinrelationtoserumCXCL7concentrationintheAp/Bcset.(I)YoudenindexintheAp/Bcset.(J)AUCintheBp/Acset.(K)SensitivityandspecificityinrelationtoserumCXCL7concentrationintheBp/Acset.(L)YoudenindexintheBp/Acset.2.5.4不同TNM分期CRC患者血清CXCL7的診斷結(jié)果

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