【摘要】：研究目的:1、研究四跨膜蛋白Tspan9對胃癌SGC7901細(xì)胞遷移和侵襲的影響;2、觀察Tspan9對整合素β1蛋白表達(dá)的影響;3、驗(yàn)證Tspan9是否通過整合素β1影響SGC7901細(xì)胞的遷移和侵襲。研究方法:1、首先檢測前期通過慢病毒轉(zhuǎn)染Tspan9的T-SGC7901細(xì)胞中Tspan9蛋白的表達(dá)情況;2、通過劃痕實(shí)驗(yàn)和transwell實(shí)驗(yàn)分別檢測Tspan9對SGC7901細(xì)胞遷移和侵襲的影響;3、通過脂質(zhì)體瞬時(shí)轉(zhuǎn)染整合素β1,建立ITG/T-SGC7901細(xì)胞,并檢測轉(zhuǎn)染后整合素β1的表達(dá)情況;4、再次通過細(xì)胞劃痕實(shí)驗(yàn)和transwell實(shí)驗(yàn)檢測轉(zhuǎn)染整合素β1后SGC7901細(xì)胞遷移和侵襲能力的變化。結(jié)果:1、與對照組相比,T-SGC7901細(xì)胞Tspan9蛋白的表達(dá)明顯增多(P0.05);2、劃痕實(shí)驗(yàn)顯示,T-SGC7901細(xì)胞的遷移率較對照組明顯降低(P0.05),transwell實(shí)驗(yàn)顯示,T-SGC7901細(xì)胞侵入到下室的細(xì)胞數(shù)量較對照組明顯減少(P0.05);3、轉(zhuǎn)染整合素β1后,ITG/T-SGC7901細(xì)胞整合素β1的表達(dá)明顯增多;4、劃痕實(shí)驗(yàn)顯示,ITG/T-SGC7901細(xì)胞的遷移率較對照組明顯加快(P0.05),transwell實(shí)驗(yàn)顯示,ITG/T-SGC7901細(xì)胞侵入到下室的細(xì)胞數(shù)量較對照組明顯增多(P0.05)。結(jié)論和意義:Tspan9能夠抑制胃癌細(xì)胞SGC7901的遷移和侵襲能力,這種抑制作用可能是通過下調(diào)整合素β1表達(dá)實(shí)現(xiàn)的。本研究進(jìn)一步加深了對四跨膜蛋白和惡性腫瘤關(guān)系的認(rèn)識,為后續(xù)研究提供實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:Aim: 1. To study the effect of four transmembrane protein Tspan9 on the migration and invasion of gastric cancer SGC7901 cells; 2, to observe the effect of Tspan9 on the expression of integrin 尾 1 protein; and 3. To verify whether Tspan9 affects the migration and invasion of SGC7901 cells through integrin 尾 1. Methods: firstly, the expression of Tspan9 protein in T-SGC7901 cells transfected with lentivirus Tspan9 was detected. 2, the effects of Tspan9 on the migration and invasion of SGC7901 cells were detected by scratch test and transwell assay, respectively. 3, ITG/T-SGC7901 cells were established by transient transfer of integrin 尾 1 by liposomes, and the expression of integrin 尾 1 after transfection was detected. 4. The migration and invasiveness of SGC7901 cells were detected by cell scratch test and transwell assay. Results: 1, compared with the control group, the expression of Tspan9 protein in T-SGC7901 cells was significantly increased (P 0.05). 2, scratch test showed that the mobility of T-SGC7901 cells was significantly lower than that in the control group (P 0.05), transwell test). 3, the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly increased after transfection of integrin 尾 1 compared with the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05). 4, scratch test showed that the mobility of ITG/T-SGC7901 cells was significantly faster than that of the control group (P 0.05), transwell test showed that the number of ITG/T-SGC7901 cells invading the inferior chamber was significantly higher than that of the control group (P 0.05). Conclusion and significance: Tspan9 can inhibit the migration and invasion of SGC7901 in gastric cancer cells, which may be achieved by down-regulating the expression of integrin 尾 1. This study further deepened the understanding of the relationship between four transmembrane proteins and malignant tumors, and provided an experimental basis for further study.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 段紀(jì)俊;嚴(yán)亞瓊;楊念念;曾晶;鄭榮壽;張思維;陳萬青;;中國惡性腫瘤發(fā)病與死亡的國際比較分析[J];中國醫(yī)學(xué)前沿雜志(電子版);2016年07期

2 Yoko Murayama;Kenji Oritani;Shusaku Tsutsui;;Novel CD9-targeted therapies in gastric cancer[J];World Journal of Gastroenterology;2015年11期

3 周蕾;武世伍;俞嵐;宋文慶;承澤農(nóng);王丹娜;;胃腺癌組織KAI1的表達(dá)及與血管、淋巴管生成的關(guān)系[J];四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2014年01期

4 張徐寧;季潤元;史恩溢;李小寧;劉海燕;戴翠萍;季寧東;;胃癌組織中MRP1/CD9、KAI1/CD82和ME491/CD63的表達(dá)及意義[J];廣東醫(yī)學(xué);2013年21期

5 ;EXPRESSION OF MASPIN AND KAI1 AND THEIR CLINICOPATHOLOGICAL SIGNIFICANCE IN CARCINOGENESIS AND PROGRESSION OF GASTRIC CANCER[J];Chinese Medical Sciences Journal;2004年03期

，

本文編號：2508183