P53蛋白與miR375在青海地區(qū)胃癌患者血漿中的水平及臨床意義
發(fā)布時間：2019-06-02 10:05
【摘要】：目的:探討青海地區(qū)胃癌患者血漿中P53蛋白與mi R375的水平及臨床意義,以了解兩者是否可以聯(lián)合作為預(yù)測胃癌發(fā)生的高特異性因子。方法:采用Elisa法及實時熒光定量PCR(RT-q PCR)檢測35例胃癌患者(GC組)、35例慢性萎縮性胃炎患者(CAG組)及35例慢性非萎縮性胃炎者(CSG組)血漿中P53蛋白與mi R375的水平;觀察不同疾病組患者P53蛋白與mi R375的水平變化情況。結(jié)果:1.血漿P53蛋白水平在CSG組、CAG組、GC組依次增高,分別為18.61±2.78、22.10±1.71、26.01±4.53(ng/ml)提示隨著病變程度的加重,P53蛋白水平升高,三組間比較差異有統(tǒng)計學(xué)意義(P0.05)。2.血漿mi R375表達水平GC組較CAG組、CSG組明顯降低,分別為1.41±0.56、2.76±0.39、2.75±0.21,與后兩組比較差異有統(tǒng)計學(xué)意義(P0.01),而后兩組之間差異無統(tǒng)計學(xué)意義(P=0.73)。3.在胃癌組中mi R375的表達量在不同腫瘤直徑、淋巴結(jié)轉(zhuǎn)移、分化程度、浸潤深度、TNM分期間均有統(tǒng)計學(xué)差異(P0.05);P53蛋白水平在不同分化程度、不同浸潤深度之間均有統(tǒng)計學(xué)差異(P0.05)。4.GC組血漿P53蛋白與mi R375間呈負相關(guān)(r=-0.575 P=0.026)。結(jié)論:1.與C SG組及C AG組相比,GC組中m i R375表達降低。而P53蛋白在C SG組、C AG組、G C組依次增高,提示病變程度的加重。2.聯(lián)合檢測血漿中p53蛋白與m i R375的水平可能對胃癌的診斷具有一定參考意義。
[Abstract]:Objective: to investigate the level and clinical significance of plasma p53 protein and mi R375 in patients with gastric cancer in Qinghai Province, and to explore whether the combination of p53 protein and R375 can be used as a high specific factor to predict the occurrence of gastric cancer. Methods: 35 patients with gastric cancer (GC group) were detected by Elisa and real-time fluorescence quantitative PCR (RT-q PCR). The levels of p53 protein and mi R375 in plasma of 35 patients with chronic atrophic gastritis (CAG group) and 35 patients with chronic non-atrophic gastritis (CSG group). The changes of p53 protein and mi R375 in different disease groups were observed. Result: 1. The plasma p53 protein levels in CSG group, CAG group and GC group were 18.61 鹵2.78, 22.10 鹵1.71 and 26.01 鹵4.53 (ng/ml), respectively, suggesting that the level of p53 protein increased with the severity of the lesion. There was significant difference among the three groups (P 0.05). 2. The expression level of plasma mi R375 in GC group was significantly lower than that in CAG group and CSG group, which was 1.41 鹵0.56, 2.76 鹵0.39 and 2.75 鹵0.21, respectively, which was significantly different from that in the latter two groups (P 0.01). There was no significant difference between the latter two groups (P 鈮，
本文編號：2491010
[Abstract]:Objective: to investigate the level and clinical significance of plasma p53 protein and mi R375 in patients with gastric cancer in Qinghai Province, and to explore whether the combination of p53 protein and R375 can be used as a high specific factor to predict the occurrence of gastric cancer. Methods: 35 patients with gastric cancer (GC group) were detected by Elisa and real-time fluorescence quantitative PCR (RT-q PCR). The levels of p53 protein and mi R375 in plasma of 35 patients with chronic atrophic gastritis (CAG group) and 35 patients with chronic non-atrophic gastritis (CSG group). The changes of p53 protein and mi R375 in different disease groups were observed. Result: 1. The plasma p53 protein levels in CSG group, CAG group and GC group were 18.61 鹵2.78, 22.10 鹵1.71 and 26.01 鹵4.53 (ng/ml), respectively, suggesting that the level of p53 protein increased with the severity of the lesion. There was significant difference among the three groups (P 0.05). 2. The expression level of plasma mi R375 in GC group was significantly lower than that in CAG group and CSG group, which was 1.41 鹵0.56, 2.76 鹵0.39 and 2.75 鹵0.21, respectively, which was significantly different from that in the latter two groups (P 0.01). There was no significant difference between the latter two groups (P 鈮，
本文編號：2491010
本文鏈接：http://sikaile.net/yixuelunwen/zlx/2491010.html
最近更新
教材專著
