二甲雙胍對(duì)肝癌荷瘤鼠抗腫瘤過(guò)程中AMPK、RAGE表達(dá)變化研究
發(fā)布時(shí)間：2019-05-29 00:25
【摘要】：目的:運(yùn)用動(dòng)物實(shí)驗(yàn)探討二甲雙胍對(duì)肝癌荷瘤鼠的抗腫瘤機(jī)制,并對(duì)傳統(tǒng)觀點(diǎn)的AMPK(adenosine monphosphate activated protein Kinase腺苷酸活化蛋白激酶)表達(dá)進(jìn)行檢測(cè),同時(shí)檢測(cè)AGEs/RAGE(advanced glycation end products/Receptor for advanced glycation end products糖基化終末產(chǎn)物/糖基化終末產(chǎn)物受體)通路的相關(guān)指標(biāo),研究?jī)烧咧g可能的聯(lián)系。方法:1、利用BALB/C裸鼠左側(cè)腋下進(jìn)行皮下HepG2肝癌細(xì)胞接種,制作裸鼠荷瘤模型,造模成功后隨機(jī)分組進(jìn)行空白對(duì)照,BSA,二甲雙胍,AGEs-BSA,二甲雙胍及AGEs-BSA兩者聯(lián)合等干預(yù)。2、定期測(cè)定裸鼠體重變化,測(cè)定空腹血糖,測(cè)量腫瘤體積的長(zhǎng)短徑a,b數(shù)值。3、經(jīng)過(guò)4周的干預(yù),取靜脈血樣本,靜置過(guò)夜,離心1000轉(zhuǎn),20分鐘后,取上清液-80℃保存,取血樣本后,處死裸鼠,取肝細(xì)胞癌組織,正常肝組織,瘤旁組織于-80℃保存。4、運(yùn)用ELISA法測(cè)定裸鼠血清中AGEs濃度,免疫組化法測(cè)定各組織中AMPK,RAGE等表達(dá)。結(jié)果:1、二甲雙胍具有抑制腫瘤生長(zhǎng)作用,能夠減少荷瘤鼠體重(P0.05),對(duì)荷瘤鼠的空腹血糖無(wú)明顯影響(P0.05)。2、ELISA測(cè)得注射AGEs的裸鼠,注射AGEs并聯(lián)用二甲雙胍的裸鼠血清中可測(cè)得含量較低的AGEs,其他組低于檢測(cè)值下限。3、運(yùn)用免疫組化法進(jìn)行AMPK,RAGE檢測(cè),可發(fā)現(xiàn)二甲雙胍可升高AMPK表達(dá),降低RAGE表達(dá)。AGEs對(duì)于RAGE存在影響。結(jié)論:二甲雙胍對(duì)于肝癌裸鼠荷瘤模型有抗腫瘤作用,可減輕裸鼠體重,對(duì)于空腹血糖無(wú)明顯影響�？赡芡ㄟ^(guò)激活A(yù)MPK途徑,下調(diào)RAGE得以發(fā)揮抗腫瘤作用,長(zhǎng)時(shí)間的干預(yù)下,AGEs可能存在降低RAGE表達(dá),提高AMPK表達(dá)的作用,AMPK與RAGE可能存在一定聯(lián)系,為二甲雙胍抗腫瘤機(jī)制提供了新的思路。
[Abstract]:Objective: to investigate the anti-tumor mechanism of metformin in tumor-bearing mice with liver cancer and to detect the expression of AMPK (adenosine monphosphate activated protein Kinase adenylate activated protein kinase (adenylate activated protein kinase) from the traditional point of view. At the same time, the related indexes of AGEs/RAGE (advanced glycation end products/Receptor for advanced glycation end products glycosylation end product / glycosylation end product receptor pathway were detected, and the possible relationship between them was studied. Methods: 1. Subcutaneous HepG2 liver cancer cells were inoculated under the left armpit of BALB/C nude mice to establish tumor-bearing model in nude mice. After successful modeling, they were randomly divided into blank control, BSA, metformin, AGEs-BSA,. Metformin and AGEs-BSA combined intervention. 2. The body weight of nude mice was measured regularly, fasting blood glucose was measured, and the long and short diameter a, b value of tumor volume was measured. 3. After 4 weeks of intervention, venous blood samples were taken and put overnight. After 20 minutes of centrifugation, the culture medium was preserved at-80 鈩，
本文編號(hào)：2487494
[Abstract]:Objective: to investigate the anti-tumor mechanism of metformin in tumor-bearing mice with liver cancer and to detect the expression of AMPK (adenosine monphosphate activated protein Kinase adenylate activated protein kinase (adenylate activated protein kinase) from the traditional point of view. At the same time, the related indexes of AGEs/RAGE (advanced glycation end products/Receptor for advanced glycation end products glycosylation end product / glycosylation end product receptor pathway were detected, and the possible relationship between them was studied. Methods: 1. Subcutaneous HepG2 liver cancer cells were inoculated under the left armpit of BALB/C nude mice to establish tumor-bearing model in nude mice. After successful modeling, they were randomly divided into blank control, BSA, metformin, AGEs-BSA,. Metformin and AGEs-BSA combined intervention. 2. The body weight of nude mice was measured regularly, fasting blood glucose was measured, and the long and short diameter a, b value of tumor volume was measured. 3. After 4 weeks of intervention, venous blood samples were taken and put overnight. After 20 minutes of centrifugation, the culture medium was preserved at-80 鈩，
本文編號(hào)：2487494
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