【摘要】：第一部分 Ulk2過表達(dá)對肺癌細(xì)胞細(xì)胞系A(chǔ)549、H460自噬水平的影響目的：研究Ulk2過表達(dá)對非小細(xì)胞肺癌細(xì)胞系(A549和H460)細(xì)胞自噬水平的影響。方法：培養(yǎng)A549和H460細(xì)胞,將表達(dá)Ulk2過表達(dá)質(zhì)粒和空載質(zhì)粒轉(zhuǎn)染A549和H460細(xì)胞,使用western blot驗證質(zhì)粒轉(zhuǎn)染結(jié)果,并檢測細(xì)胞自噬相關(guān)標(biāo)記物L(fēng)C3 Ⅱ和p62的表達(dá)量。結(jié)果：轉(zhuǎn)染Ulk2過表達(dá)質(zhì)粒后,兩種細(xì)胞Ulk2的表達(dá)量相對轉(zhuǎn)染空載質(zhì)粒的對照組提升,并且細(xì)胞自噬相關(guān)標(biāo)記物L(fēng)C3 Ⅱ表達(dá)量明顯升高,p62的表達(dá)量明顯降低,結(jié)果具有統(tǒng)計學(xué)意義(p0.05)。結(jié)論：轉(zhuǎn)染Ulk2過表達(dá)質(zhì)粒可以增強(qiáng)非小細(xì)胞肺癌細(xì)胞系(A549和H460)細(xì)胞自噬水平。第二部分 UIk2過表達(dá)對肺癌細(xì)胞的增殖能力、對藥物敏感性及細(xì)胞凋亡的影響目的：研究Ulk2過表達(dá)對非小細(xì)胞肺癌細(xì)胞系(A549和H460)增殖及化療敏感性的影響。方法：培養(yǎng)A549和H460細(xì)胞,將表達(dá)Ulk2過表達(dá)質(zhì)粒和空載質(zhì)粒轉(zhuǎn)染A549和H460細(xì)胞,MTT法檢測細(xì)胞增殖和對化療藥物的敏感性,流式細(xì)胞術(shù)和Western blot檢測細(xì)胞凋亡。結(jié)果：與對照組相比,Ulk2過表達(dá)后A549和H460的細(xì)胞增殖能力明顯降低(P0.05),對順鉑和依托泊苷的敏感性明顯上升(P0.05),順鉑處理后細(xì)胞凋亡比率增加,切割后的Caspase3和PARP明顯升高。結(jié)論：U1k2過表達(dá)能顯著抑制非小細(xì)胞肺癌細(xì)胞系增殖,增強(qiáng)化療敏感性。
[Abstract]:Part one: effects of Ulk2 overexpression on autophagy in lung cancer cell lines A549 and H460 objective: to study the effect of Ulk2 overexpression on autophagy in non-small cell lung cancer cell lines A549 and H460. Methods: A549 and H460 cells were cultured and transfected with Ulk2 overexpression plasmid and empty plasmid. The results were verified by western blot, and the expression levels of LC3 鈪，

本文編號：2452118