【摘要】：目的研究EZH2蛋白抑制劑DZNep對(duì)前列腺癌PC3細(xì)胞增殖的影響及其用于人前列腺癌治療的潛在價(jià)值。方法 MTT分析DZNep處理、siRNA敲低EZH2和EZH2過(guò)表達(dá)對(duì)PC3細(xì)胞增殖的影響,Western blot檢測(cè)EZH2和Bax/Bcl-2的表達(dá)。結(jié)果 DZNep抑制PC-3細(xì)胞增殖、誘導(dǎo)細(xì)胞凋亡,上調(diào)Bax基因表達(dá)并下調(diào)Bcl-2基因表達(dá)。敲低EZH2抑制PC3細(xì)胞增殖,過(guò)表達(dá)EZH2則拮抗DZNep對(duì)PC3細(xì)胞的增殖抑制。結(jié)論 DZNep通過(guò)下調(diào)EZH2表達(dá)、誘導(dǎo)細(xì)胞凋亡發(fā)生從而抑制PC3細(xì)胞增殖。DZNep具有抗人前列腺癌治療的潛在應(yīng)用前景。
[Abstract]:Objective to investigate the effect of EZH2 protein inhibitor DZNep on proliferation of prostate cancer PC3 cells and its potential value in the treatment of human prostate cancer. Methods MTT was used to analyze the effects of DZNep treatment, siRNA knockdown EZH2 and EZH2 overexpression on the proliferation of PC3 cells., Western blot was used to detect the expression of EZH2 and Bax/Bcl-2. Results DZNep inhibited the proliferation of PC-3 cells, induced apoptosis, up-regulated the expression of Bax gene and down-regulated the expression of Bcl-2 gene. Low level of EZH2 inhibited the proliferation of PC3 cells, and over-expression of EZH2 inhibited the proliferation of PC3 cells induced by DZNep. Conclusion DZNep can inhibit the proliferation of PC3 cells by down-regulating the expression of EZH2 and inducing apoptosis. DZNep has the potential application prospect of anti-prostate cancer therapy.
【作者單位】： 三峽大學(xué)醫(yī)學(xué)院/腫瘤微環(huán)境與免疫治療湖北省重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助(編號(hào):81372265) 腫瘤微環(huán)境與免疫治療湖北省重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金(編號(hào):2016KZL01,2016KZL10)
【分類(lèi)號(hào)】：R737.25
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本文編號(hào)：2448195