【摘要】：目的探討腫瘤抑制因子(Kiss)-1在胃腺癌組織中的表達(dá)及對(duì)胃腺癌細(xì)胞增殖、遷移能力的影響。方法 qRT-PCR檢測(cè)50例胃腺癌組織和對(duì)應(yīng)的癌旁組織中Kiss-1水平。分別將Kiss-1小干擾RNA(siRNA Kiss-1)和對(duì)照小RNA(siRNA control)、Kiss-1過(guò)表達(dá)載體(p EGFP-N1-Kiss-1)和對(duì)照空載體(p EGFP-N1)轉(zhuǎn)染至胃腺癌細(xì)胞株(MKN-45)中,培養(yǎng)48 h后,Western印跡檢測(cè)細(xì)胞中Kiss-1、基質(zhì)金屬蛋白酶(MMP)-9、MMP-2、β-連環(huán)蛋白(β-catenin)、C-myc蛋白水平,噻唑藍(lán)(MTT)檢測(cè)細(xì)胞增殖能力,細(xì)胞劃痕實(shí)驗(yàn)檢測(cè)細(xì)胞遷移能力。用Wnt/β-catenin信號(hào)通路抑制劑FH535作用胃腺癌細(xì)胞后,檢測(cè)細(xì)胞增殖和凋亡情況。結(jié)果 Kiss-1在胃腺癌組織中的表達(dá)水平顯著低于癌旁組織(P0.01)。p EGFP-N1-Kiss-1組細(xì)胞存活率和遷移率顯著低于p EGFP-N1組,siRNA Kiss-1組顯著高于siRNA control組(P0.01)。p EGFP-N1-Kiss-1組細(xì)胞中MMP-9、MMP-2、β-catenin、C-myc水平顯著低于p EGFP-N1組,siRNA Kiss-1組顯著高于siRNA control組(P0.01)。抑制劑作用后的胃腺癌細(xì)胞增殖遷移趨勢(shì)與p EGFP-N1-Kiss-1組一致。結(jié)論 Kiss-1在胃腺癌組織中低表達(dá),Kiss-1通過(guò)Wnt/β-catenin信號(hào)通路抑制胃腺癌細(xì)胞增殖遷移能力。
[Abstract]:Objective to investigate the expression of tumor suppressor (Kiss)-1 in gastric adenocarcinoma and its effect on proliferation and migration of gastric adenocarcinoma cells. Methods qRT-PCR was used to detect the level of Kiss-1 in 50 gastric adenocarcinoma tissues and corresponding paracancerous tissues. Kiss-1 small interfering RNA (siRNA Kiss-1, control small RNA (siRNA control), Kiss-1 overexpression vector (p EGFP-N1-Kiss-1) and control empty vector (p EGFP-N1) were transfected into gastric adenocarcinoma cell line (MKN-45), respectively. After 48 hours of culture, the levels of Kiss-1, matrix metalloproteinases (MMP)-9, MMP-2, 尾-catenin), C-myc (尾-catenin), C-myc protein) in the cells were detected by Western blot, and the proliferation ability of the cells was detected by thiazolyl blue (MTT). The ability of cell migration was detected by cell scratch test. Wnt/ 尾-catenin signaling pathway inhibitor FH535 was used to detect the proliferation and apoptosis of gastric adenocarcinoma cells. Results the expression level of Kiss-1 in gastric adenocarcinoma tissues was significantly lower than that in paracancerous tissues (P0.01). The cell survival rate and migration rate in). P EGFP-N1-Kiss-1 group were significantly lower than those in p-EGFP-N1 group. The level of MMP-9,MMP-2, 尾-catenin,C-myc in siRNA Kiss-1 group was significantly higher than that in siRNA control group (P0.01), and the level of MMP-9,MMP-2, 尾-catenin,C-myc in siRNA Kiss-1 group was significantly higher than that in siRNA control group (P0.01). The proliferation and migration trend of gastric adenocarcinoma cells treated with inhibitor was consistent with that of p-EGFP-N1-Kiss-1 group. Conclusion the expression of Kiss-1 is low in gastric adenocarcinoma tissues. Kiss-1 inhibits the proliferation and migration of gastric adenocarcinoma cells through Wnt/ 尾-catenin signaling pathway.
【作者單位】： 河北醫(yī)科大學(xué)第一醫(yī)院病理科;河北醫(yī)科大學(xué)第一醫(yī)院骨科;
【基金】：河北省衛(wèi)計(jì)委青年科技課題(20150642)
【分類(lèi)號(hào)】：R735.2

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