【摘要】：目的探討冬凌草甲素對人急性T淋巴細(xì)胞白血病Jurkat細(xì)胞的作用及其機(jī)制。方法體外培養(yǎng)Jurkat細(xì)胞株,用不同濃度冬凌草甲素(0、1.25、2.5、5和10μmol/L)作用Jurkat細(xì)胞不同時(shí)間(24、48、72 h),MTT實(shí)驗(yàn)觀察細(xì)胞增殖情況,熒光顯微鏡觀察不同濃度冬凌草甲素處理Jurkat細(xì)胞12 h后的細(xì)胞核形態(tài)變化。采用Western blot半定量法檢測不同濃度冬凌草甲素作用Jurkat細(xì)胞24 h,以及5μmol/L冬凌草甲素處理Jurkat細(xì)胞不同時(shí)間(0、2、6、12和24 h)后的Brg1、P53和C-myc蛋白表達(dá)。用si RNA沉默Jurkat細(xì)胞的Brg1,觀察其對P53、C-myc蛋白表達(dá)以及對增殖的影響。結(jié)果與無處理組相比,冬凌草甲素對Jurkat細(xì)胞增殖有抑制作用(P0.05),且呈濃度和時(shí)間依賴性;熒光顯微鏡下發(fā)現(xiàn),冬凌草甲素處理后的Jurkat細(xì)胞出現(xiàn)細(xì)胞核濃集、固縮等典型凋亡形態(tài)變化。與無處理組相比,5μmol/L冬凌草甲素作用下,Jurkat細(xì)胞的Brg1和C-myc表達(dá)下降,而P53表達(dá)上升。si RNA沉默Jurkat細(xì)胞的Brg1后,P53表達(dá)升高,C-myc蛋白表達(dá)下降,細(xì)胞生長明顯受抑(P0.05)。結(jié)論冬凌草甲素對Jurkat細(xì)胞生長具有抑制作用,其機(jī)制可能通過影響B(tài)rg1信號通路起作用。
[Abstract]:Objective to investigate the effect and mechanism of Oridonin on human acute T lymphocyte leukemia (Jurkat) cells. Methods Jurkat cells were cultured in vitro. Jurkat cells were treated with different concentrations of oridonin (0, 1.25, 2.5, 5 and 10 渭 mol / L) for different time (24,48,72 h), MTT). The nuclear morphology of Jurkat cells treated with different concentrations of Oridonin for 12 h was observed by fluorescence microscope. The expression of Brg1,P53 and C-myc proteins in Jurkat cells treated with 5 渭 mol / L oridonin for 24 h (0, 2, 6, 12 and 24 h) was detected by Western blot semi-quantitative method. The effects of si RNA on the expression of P53, c-myc protein and the proliferation of Jurkat cells were observed by Brg1, silencing. Results compared with the non-treated group, oridonin inhibited the proliferation of Jurkat cells (P0.05) in a concentration-and time-dependent manner. The typical apoptotic morphological changes of Jurkat cells treated with Oridonin were observed under fluorescence microscope, such as nucleus concentration, condensation and so on. Compared with the non-treated group, the expression of Brg1 and C-myc in Jurkat cells decreased and the expression of p53 increased in Jurkat cells treated with 5 渭 mol / L oridonin, but the expression of p53 increased and the expression of C-myc protein decreased in Jurkat cells after silencing Jurkat cells with si-RNA. Cell growth was significantly inhibited (P0.05). Conclusion Aconitine can inhibit the growth of Jurkat cells, and its mechanism may play an important role in the Brg1 signaling pathway. [WT5 "HZ] conclusion [WT5" BZ]
【作者單位】： 皖南醫(yī)學(xué)院弋磯山醫(yī)院兒科;皖南醫(yī)學(xué)院弋磯山醫(yī)院放療科;皖南醫(yī)學(xué)院弋磯山醫(yī)院內(nèi)分泌科;
【基金】：國家自然科學(xué)基金青年項(xiàng)目(81600645) 皖南醫(yī)學(xué)院中青年科研基金(WK2016F17)
【分類號】：R733.71

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