【摘要】：目的研究急性早幼粒細(xì)胞白血病(APL)表型特征及臨床意義。方法采用流式細(xì)胞術(shù)四色方案對(duì)APL進(jìn)行免疫分型,以CD45/SSC-lin圖定位白血病細(xì)胞,依據(jù)抗原表達(dá)強(qiáng)度將抗原表達(dá)劃分為五級(jí)分析。結(jié)果典型APL細(xì)胞抗原表達(dá)強(qiáng)度及所占比例依次為:CD33(100%)、髓過氧化物酶(MPO)100%、CD38(82.35%)、CD13(64.71%)、CD64(50%)、CD123(47.06)、CD117(44.12%),HLA-DR和CD34基本不表達(dá);伴淋系表達(dá)APL患者化療后緩解率明顯低于陰性APL患者。結(jié)論 APL免疫分型典型表型特征為高側(cè)向角散射(high SSC)、CD33~+(Ⅰ級(jí))、CD38~+(Ⅰ級(jí))、MPO~+(Ⅰ級(jí))、CD13~+(Ⅲ級(jí))、CD64~+(Ⅰ級(jí)/Ⅲ級(jí))、CD117~+(Ⅱ級(jí)/Ⅲ級(jí)/Ⅳ級(jí))、CD123~+(Ⅲ級(jí)/Ⅳ級(jí))、CD11b~-、HLA-DR~-、CD34~-。掌握APL免疫表型特征有助于APL快速診斷,且對(duì)MRD檢測(cè)有指導(dǎo)意義。
[Abstract]:Objective to study the phenotypic characteristics and clinical significance of (APL) in acute promyelocytic leukemia. Methods Immunophenotyping of APL was carried out by flow cytometry, and the leukemic cells were located by CD45/SSC-lin, and the antigen expression was divided into five stages according to the intensity of antigen expression. Results the antigen expression intensity and percentage of typical APL cells were as follows: CD33 (100%), myeloperoxidase (MPO) 100 (82.35%), CD13 (64.71%), CD64 (50%), CD123 (47.06), CD117 (44.12%). HLA-DR and CD34 were not expressed. The remission rate after chemotherapy in patients with lymphoid expression of APL was significantly lower than that in patients with negative APL. Conclusion the typical phenotypic features of APL immunophenotype are high lateral angle scattering (high SSC), CD33~ (鈪，

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