【摘要】：目的:構(gòu)造SD大鼠肝癌模型,用β-catenin抑制劑ICG-001活體干預(yù)已成瘤的SD大鼠,探討β-catenin抑制劑ICG-001對(duì)SD大鼠肝癌組織中凋亡抑制蛋白Survivin的影響。方法:予二乙基亞硝胺(DEN)喂養(yǎng)SD大鼠,隨后將已成瘤的大鼠分成腫瘤組,DMSO組和給藥組。給藥組大鼠予ICG-001腹腔注射抑制β-catenin的表達(dá)后,Western blot檢測(cè)ICG-001對(duì)大鼠肝癌組織中Survivin蛋白表達(dá)的影響,半定量RT-PCR檢測(cè)ICG-001對(duì)大鼠肝癌組織中Survivin的mRNA的影響。分別用Image J軟件和凝膠成像分析系統(tǒng)檢測(cè)Survivin的蛋白和mRNA表達(dá)水平,測(cè)量條帶的灰度值,將其轉(zhuǎn)化為數(shù)據(jù),并使用SPSS.18進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:予二乙基亞硝胺喂養(yǎng)SD大鼠,構(gòu)造肝癌模型成功,成瘤率約為83.3%。用ICG-001抑制β-catenin的表達(dá)后,Western blot結(jié)果顯示:給藥組Survivin的表達(dá)明顯低于對(duì)照組,且差異有統(tǒng)計(jì)學(xué)意義,p=0.0010.05;而對(duì)照組和DMSO組組間差異沒(méi)有統(tǒng)計(jì)學(xué)意義,p=0.4280.05;即ICG-001給藥后Survivin的蛋白表達(dá)量降低。半定量RT-PCR結(jié)果顯示:給藥組Survivin的mRNA表達(dá)含量較對(duì)照組明顯減低,且差異有統(tǒng)計(jì)學(xué)意義:p=0.0360.05;而對(duì)照組和DMSO組組間差異沒(méi)有統(tǒng)計(jì)學(xué)意義,p=0.4560.05;即ICG-001給藥后Survivin的mRNA表達(dá)量降低。結(jié)論:予二乙基亞硝胺喂養(yǎng)SD大鼠,構(gòu)造肝癌模型成功,成瘤率約為83.3%。β-catenin抑制劑ICG-001明顯抑制SD大鼠肝癌中Survivin的蛋白表達(dá)及mRNA表達(dá)。
[Abstract]:Aim: to construct a SD rat model of hepatocellular carcinoma, and to investigate the effect of 尾 catenin inhibitor ICG-001 on apoptosis inhibitor Survivin in SD rat liver cancer tissue by using 尾-catenin inhibitor ICG-001 in vivo to interfere with SD rats. Methods: SD rats were fed with diethylnitrosamine (DEN), then the rats were divided into three groups: tumor group, DMSO group and administration group. After intraperitoneal injection of ICG-001 to inhibit the expression of 尾-catenin, Western blot was used to detect the effect of ICG-001 on the expression of Survivin protein in rat liver cancer, and semi-quantitative RT-PCR was used to detect the effect of ICG-001 on Survivin mRNA in rat liver cancer. The protein and mRNA expression levels of Survivin were detected by Image J software and gel imaging analysis system. The gray values of the bands were measured and converted into data. The results were analyzed statistically by SPSS.18. Results: SD rats were fed with diethylnitrosamine and the liver cancer model was successfully constructed. The tumorigenesis rate was about 83.3%. The results of, Western blot showed that the expression of 尾-catenin in the administration group was significantly lower than that in the control group, and the difference was statistically significant (p0. 0010.05), but there was no significant difference between the control group and the DMSO group (p0. 428. 05). That is, the protein expression of Survivin decreased after ICG-001 administration. The results of semi-quantitative RT-PCR showed that the mRNA expression of Survivin in the administration group was significantly lower than that in the control group, and the difference was statistically significant: p0. 036. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 0. 05. That is, the mRNA expression of Survivin decreased after ICG-001 administration. Conclusion: SD rats were fed with diethylnitrosamine and the tumor formation rate was about 83.3%. 尾-catenin inhibitor ICG-001 significantly inhibited the expression of Survivin protein and mRNA in SD rats.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R735.7;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Jin-Ping Li;Guang-Long Feng;Da-Qing Li;Hai-Bo Wang;De-Li Zhao;Yong Wan;Hui-Jie Jiang;;Detection and differentiation of early hepatocellular carcinoma from cirrhosis using CT perfusion in a rat liver model[J];Hepatobiliary & Pancreatic Diseases International;2016年06期

2 柴紅濤;唐恩奇;李濱;蔣歡歡;張劍波;劉艷華;汪麗燕;;N-亞硝基二乙胺(DEN)誘導(dǎo)SD大鼠肝癌模型的研究[J];安徽醫(yī)學(xué);2016年04期

3 Javier Tejeda-Maldonado;Ignacio García-Juárez;Jonathan Aguirre-Valadez;Adrián González-Aguirre;Mario Vilatobá-Chapa;Alejandra Armengol-Alonso;Francisco Escobar-Penagos;Aldo Torre;Juan Francisco Sánchez-ávila;Diego Luis Carrillo-Pérez;;Diagnosis and treatment of hepatocellular carcinoma: An update[J];World Journal of Hepatology;2015年03期

4 孫雯;曹驥;盧曉旭;朱伶群;楊春;歐超;駱成飄;李瑗;蘇建家;;RNAi沉默MCM7基因?qū)θ烁伟┘?xì)胞SMMC-7721裸鼠移植瘤影響研究[J];中華腫瘤防治雜志;2015年01期

5 胡高裕;黃桂柳;黃贊松;周喜漢;胡靜;黃炳臣;;人肝癌細(xì)胞裸鼠移植瘤模型建立及苦參堿的抑瘤作用[J];世界華人消化雜志;2014年33期

6 劉東璞;盧鳳美;姚海濤;孟慶媛;盛延良;尹興忠;王抒;;黃曲霉毒素B1誘導(dǎo)大鼠肝癌模型的建立[J];黑龍江醫(yī)藥科學(xué);2012年06期

7 Clare E Hughes;Bruce Caterson;;Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues[J];World Journal of Gastroenterology;2012年30期

8 朱艷志;孔憲炳;顏朗;;間斷給藥DEN誘導(dǎo)大鼠肝癌模型建立及病理研究[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2010年12期

9 周倜;陳勇;尤楠;董曉平;張福琴;;二乙基亞硝胺誘導(dǎo)大鼠肝癌模型的建立及評(píng)價(jià)[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2010年20期

10 朱峰;楊小偉;湯永輝;羅天平;段云飛;;Walker-256大鼠移植性肝癌模型探討[J];醫(yī)學(xué)研究雜志;2010年09期

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