抑制Sema 4D表達介導(dǎo)的成骨前體細胞AKT磷酸化水平上調(diào)在乳腺癌骨轉(zhuǎn)移中的作用
[Abstract]:Objective to investigate the role of inhibiting the up-regulation of AKT phosphorylation of osteoblasts mediated by Sema 4 D in bone metastasis of breast cancer. Methods stable human breast cancer MCF-7 cell line with low expression of Sema 4D was constructed by siRNA technique. The expression of Sema 4D mRNA was detected by real-time fluorescence quantitative PCR, the proliferation ability of each group was detected by MTT assay, and the invasion ability of each group was detected by Transwell assay. The co-culture system of osteoblasts and breast cancer cells was established by Transwell chamber. The bone mineralization ability was detected by alizarin red staining. The expression of AKT,p-AKT protein was detected by, Western blot. Results the relative expression of Sema 4D mRNA in MCF-7 cells was significantly higher than that in Hs578Bst cells (P0.05). After transfection with). Sema 4D siRNA, the relative expression of Sema 4D mRNA in Sema 4D siRNA group was significantly lower than that in Control group and NC group (P0.05). There was no significant difference in the relative expression of Sema 4D mRNA between Control group and NC group (P0.05). The cell proliferation rate of). Sema 4D siRNA group was significantly lower than that of NC group and Control group (P0.05). The cell invasion rate of Sema 4D siRNA group was significantly lower than that of NC group and Control group (P0.05). The bone nodule area in MCF-7 OM group and Sema 4D siRNA OM group was significantly lower than that in Control OM group (P0.05). The area of bone nodules in Sema 4D siRNA OM group was significantly higher than that in MCF-7 OM group (P0.05). There was no significant difference in AKT protein expression level between). Control OM group, MCF-7 OM group and Sema 4D siRNA OM group (P0.05). The expression of p-AKT protein in MCF-7 OM group and Sema 4D siRNA OM group was significantly lower than that in Control OM group (P0.05), and the p-AKT protein expression level in Sema4D siRNA OM group was significantly higher than that in MCF-7 OM group (P0.05). Conclusion the expression of MCF-7 Sema4D in human breast cancer cell line is significantly up-regulated, which is related to the biological behaviors such as cell proliferation and invasion, and down-regulating the expression of Sema _ 4D can increase the level of AKT phosphorylation and attenuate the inhibition of osteoblast differentiation.
【作者單位】： 河南省人民醫(yī)院骨科;
【基金】：河南省重大科技攻關(guān)項目(No.142102310081)
【分類號】：R737.9
【相似文獻】
相關(guān)期刊論文 前10條
1 劉燕,權(quán)瑞;乳腺癌骨轉(zhuǎn)移的放射治療35例[J];中國腫瘤臨床與康復(fù);2000年03期
2 陳輝利,趙青梅,杜光祖;乳腺癌骨轉(zhuǎn)移3年以上存活病例分析[J];華南國防醫(yī)學雜志;2000年01期
3 巨愛平;乳腺癌骨轉(zhuǎn)移12例綜合治療臨床觀察[J];臨床腫瘤學雜志;2001年01期
4 蔣國勤;乳腺癌骨轉(zhuǎn)移模型的建立[J];中國普外基礎(chǔ)與臨床雜志;2001年05期
5 王躍珍,孫曉江,劉林林,孫寶勝,呂鵬;208例乳腺癌骨轉(zhuǎn)移相關(guān)因素分析[J];中國實驗診斷學;2004年04期
6 柴楓;;乳腺癌骨轉(zhuǎn)移86例回顧性分析[J];實用醫(yī)藥雜志;2006年05期
7 徐海濱;胡祖健;胡望華;史紅仙;何俊玲;黃建;;乳腺癌骨轉(zhuǎn)移綜合治療初探[J];中華乳腺病雜志(電子版);2007年01期
8 葉謙;;100例乳腺癌患者核素骨顯像結(jié)果分析[J];實用醫(yī)技雜志;2007年09期
9 周圍;周恩相;王澤強;郭瓊;;乳腺癌骨轉(zhuǎn)移57例回顧性分析[J];實用醫(yī)藥雜志;2007年05期
10 鄔健明;張勇;王偉;;乳腺癌骨轉(zhuǎn)移致股骨頸病理性骨折1例報告[J];西南軍醫(yī);2008年03期
相關(guān)會議論文 前10條
1 魏志真;董峰;;乳腺癌骨轉(zhuǎn)移的臨床特點分析[A];中華醫(yī)學會放射醫(yī)學與防護學分會第三次全中國青年學術(shù)交流會論文摘要匯編[C];2001年
2 魏志真;董峰;;乳腺癌骨轉(zhuǎn)移的臨床特點分析[A];中華醫(yī)學會放射醫(yī)學與防護學分會第三次全國中青年學術(shù)交流會論文匯編[C];2001年
3 徐海濱;胡祖健;胡望華;史紅仙;何俊玲;黃建;;乳腺癌骨轉(zhuǎn)移綜合治療初探[A];2005年浙江省外科學術(shù)會議論文匯編[C];2005年
4 蔡鋼;陳佳藝;朱驥;俞曉立;章真;郭小毛;胡超蘇;;乳腺癌骨轉(zhuǎn)移的放射治療和預(yù)后因素[A];2007第六屆全國放射腫瘤學學術(shù)年會論文集[C];2007年
5 蔡鋼;陳佳藝;朱驥;俞曉立;章真;郭小毛;胡超蘇;;乳腺癌骨轉(zhuǎn)移的放射治療和預(yù)后因素[A];2007第六屆全國放射腫瘤學學術(shù)年會論文集[C];2007年
6 劉兆U，
本文編號：2350402
本文鏈接：http://sikaile.net/yixuelunwen/zlx/2350402.html
