【摘要】：目的:化療是治療肺癌的關(guān)鍵手段之一,對(duì)化療療效的評(píng)價(jià)非常重要,Pro GRP和NSE在SCLC中都有不同程度表達(dá),本實(shí)驗(yàn)旨在研究SCLC患者化療前后血清Pro GRP及NSE水平變化,探討Pro GRP和NSE是否有望成為預(yù)測(cè)SCLC化療療效的可靠指標(biāo)。方法:健康對(duì)照組來(lái)自弋磯山醫(yī)院體檢中心,SCLC患者經(jīng)病理學(xué)組織確診,存在可評(píng)價(jià)病灶,符合化療適應(yīng)證。SCLC患者化療前1天及化療2、4周期后20天和健康對(duì)照組抽取靜脈血液3 m L,離心取上層血清于零下80℃下凍存。使用ELISA方法測(cè)定血清Pro GRP和NSE水平在化療前后的變化。結(jié)果:一、(1)與對(duì)照組比較,SCLC患者化療前與化療2周期后Pro GRP、NSE濃度明顯高于正常對(duì)照組(P≤0.05);化療4周期后與對(duì)照組比較,P0.05。(2)化療前后對(duì)比,化療前分別與化療2、4周期后Pro GRP,NSE濃度比較,P≤0.05;化療2、4周期后相比,P0.05。二、(1)各組中化療前后Pro GRP比較,CR(完全緩解)、SD(穩(wěn)定)、PD(進(jìn)展)組顯示化療前和化療2、4周期后兩兩比較,P≤0.05;PR(部分緩解)組,化療前分別與化療2、4周期后相比,P≤0.05;化療2、4周期后相比,P0.05。(2)各組中化療前后NSE比較,CR、PR、SD組顯示化療前分別與2、4周期后比較,P≤0.05;化療2、4周期后相比,P0.05。PD組化療前和化療2周期后比較,化療2、4周期后相比,P≤0.05;化療前和化療4周期后相比,P0.05。三、(1)化療前4組比較,CR、PR、SD、PD組Pro GRP、NSE濃度比較,P0.05。(2)化療2周期后4組比較,CR組與PR、SD、PD組Pro GRP比較,P≤0.05;PR、SD、PD組Pro GRP比較,P0.05。CR與SD、PD組NSE比較,PR與PD組NSE比較,P≤0.05;PR與CR、SD組NSE比較,SD、PD組NSE比較,P0.05。(3)化療4周期后,CR、PR、SD組Pro GRP比較,P0.05;PD組與CR、PR、SD組比較,P≤0.05。NSE比較與化療2周期后NSE化療前后比較相似,除SD、PD組比較,P≤0.05。結(jié)論:1.化療前Pro GRP、NSE濃度明顯高于健康人群,化療后Pro GRP、NSE的濃度均有不同程度下降,以化療2周期后下降明顯。2.SCLC患者化療2、4周期后Pro GRP、NSE濃度比較,P0.05,但Pro GRP、NSE水平仍呈小幅下降趨勢(shì)。3.化療后CR、PR、SD、PD組血清Pro GRP、NSE濃度下降幅度不一,CR組下降明顯,Pro GRP對(duì)化療療效的監(jiān)測(cè)更有意義。化療前后血清Pro GRP、NSE濃度之間呈正相關(guān)。4.Pro GRP、NSE聯(lián)合檢測(cè)SCLC與單獨(dú)檢測(cè)的敏感性差異不大,特異性較單獨(dú)檢測(cè)高。
[Abstract]:Objective: chemotherapy is one of the key methods in the treatment of lung cancer. The evaluation of chemotherapy efficacy is very important for the expression of, Pro GRP and NSE in SCLC. The purpose of this study was to study the changes of serum Pro GRP and NSE levels in patients with SCLC before and after chemotherapy. To explore whether Pro GRP and NSE may be a reliable index to predict the effect of SCLC chemotherapy. Methods: the healthy control group was from the physical examination center of Gi Ji Shan Hospital. The patients with SCLC were diagnosed by pathological tissue and had evaluable lesions. According to the indication of chemotherapy, venous blood was taken from SCLC patients 1 day before chemotherapy, 20 days after 2 cycles of chemotherapy and healthy control group. The upper serum was centrifuged and frozen at minus 80 鈩，

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