【摘要】：[目的]探討雙泛素(human leukocyte antigen F-associated transcript 10,Diubiquitin,FAT10)和核因子 κ Bp50 (nuclear factor κBp50,NF-κBp50)在乳腺浸潤性導管癌中的表達情況及其臨床病理意義。[方法]本研究選取云南省腫瘤醫(yī)院2014-2015年間手術切除或穿刺的石蠟標本,其中包括112例乳腺浸潤性導管癌組織和53例癌旁組織,應用MaxvisionTM免疫組織化學的方法,檢測乳腺浸潤性導管癌癌組織和癌旁組織中FAT10與NF- κ B p50的蛋白表達情況;同時收集2016-2017年間新鮮的乳腺浸潤性導管癌組織和癌旁組織共28對,應用實時熒光定量PCR的方法,檢測乳腺浸潤性導管癌癌組織和癌旁組織中FAT10與NF- κ B p50的mRNA水平;并分別從蛋白水平和mRNA水平分析二者與乳腺浸潤性導管癌患者臨床病理因素之間的關系,以及FAT10與NF- κ B p50二者之間表達的相關性。[結(jié)果]FAT10和NF- κ Bp50蛋白在乳腺浸潤性導管癌中的陽性表達率分別為77.7%和81.3%,明顯高于癌旁組織(39.6%和45.3%),差異有顯著統(tǒng)計學意義(P0.001); FAT10和NF-κBp50mRNA在乳腺浸潤性導管癌中的相對表達量中位數(shù)分別為6.21和4.08,明顯高于癌旁組織1,差異有統(tǒng)計學意義(P0.05)。FAT10、NF- κ Bp50蛋白在有淋巴結(jié)轉(zhuǎn)移和遠處轉(zhuǎn)移的癌組織中高表達,并隨組織學分級、TNM分期的升高而表達逐漸升高,差異有統(tǒng)計學意義(P0.05),但與患者年齡、腫瘤大小及分子分型差異無統(tǒng)計學意義(P0.05)。FAT10和NF-κ Bp50的mRNA水平與患者淋巴結(jié)轉(zhuǎn)移、遠處轉(zhuǎn)移、組織學分級、TNM分期、患者年齡、腫瘤大小及分子分型間差異均無統(tǒng)計學意義(P0.05)。此外,FAT10與NF- κ Bp50兩者之間蛋白和mRNA的表達均呈正相關(r=0.621,P0.001 和 r=0.475,P=0.011)。[結(jié)論]1、FAT10、NF-κBp50在乳腺浸潤性導管癌中的表達高于癌旁組織。2、FAT10、NF-κBp50蛋白在有淋巴結(jié)轉(zhuǎn)移和遠處轉(zhuǎn)移的癌組織中高表達,并隨組織學分級、TNM分期的升高而表達升高,但與患者年齡、腫瘤大小、分子分型差異無統(tǒng)計學意義,提示二者與乳腺癌的惡性程度密切相關,可能參與促進了乳腺癌的進展。3、FAT10、NF-κ Bp50 mRNA水平與臨床病理因素之間的關系未顯示出統(tǒng)計學意義,可能與表觀調(diào)控有關,具體原因有待進一步的研究。4、FAT10、NF-xBp50二者在乳腺浸潤性導管癌中的表達呈正相關,提示二者可能在乳腺癌的發(fā)生發(fā)展中協(xié)同發(fā)揮作用。
[Abstract]:[objective] to investigate the expression and clinicopathological significance of diubiquitin (human leukocyte antigen F-associated transcript 10 diubiquitin (human leukocyte antigen F-associated transcript 10) and nuclear factor- 魏 Bp50 (nuclear factor 魏 Bp50,NF- 魏 Bp50 in breast invasive ductal carcinoma. [methods] A total of 112 breast invasive ductal carcinoma tissues and 53 paracancerous tissues were collected from Yunnan Cancer Hospital from 2014 to 2015. MaxvisionTM immunohistochemical method was used. To detect the expression of FAT10 and NF- 魏 B p50 protein in breast invasive ductal carcinoma tissues and adjacent tissues, 28 pairs of fresh breast invasive ductal carcinoma tissues and adjacent tissues were collected from 2016 to 2017, and real-time fluorescence quantitative PCR was used to detect the expression of FAT10 and NF- 魏 B p50 in breast invasive ductal carcinoma tissues and adjacent tissues. To detect the mRNA levels of FAT10 and NF- 魏 B p50 in breast invasive ductal carcinoma tissues and adjacent tissues, and to analyze the relationship between FAT10 and NF- 魏 B p50 and clinicopathological factors in breast invasive ductal carcinoma from protein level and mRNA level, respectively. And the correlation between the expression of FAT10 and NF- 魏 B p50. [results] the positive expression rates of FAT10 and NF- 魏 Bp50 in breast invasive ductal carcinoma were 77.7% and 81.3%, respectively, which were significantly higher than those in paracancerous tissues (39.6% and 45.3%), the difference was statistically significant (P0.001), and the relative expression of FAT10 and NF- 魏 Bp50mRNA in invasive ductal carcinoma of breast was significantly higher than that of adjacent tissues (39.6% and 45.3%). The median expression levels of FAT10,NF- 魏 Bp50 were 6.21 and 4.08, respectively, which were significantly higher than those in adjacent tissues (P0.05). The expression of FAT10,NF- 魏 Bp50 protein was highly expressed in cancer tissues with lymph node metastasis and distant metastasis. With the increase of histological grade and TNM stage, the expression of mRNA increased gradually, the difference was statistically significant (P0.05), but there was no significant difference with age, tumor size and molecular typing (P0.05). The mRNA levels of FAT10 and NF- 魏 Bp50 were correlated with lymph node metastasis and distant metastasis. There was no significant difference in histological grade, TNM stage, age, tumor size and molecular typing between patients (P0.05). In addition, there was a positive correlation between the expression of protein and mRNA between FAT10 and NF- 魏 Bp50 (r = 0.621, P 0.001, r = 0.475, P = 0.011). [conclusion] 1 the expression of NF- 魏 Bp50 in breast invasive ductal carcinoma is higher than that in adjacent breast tissue. 2FAT10NF-kappa Bp50 protein is highly expressed in cancer with lymph node metastasis and distant metastasis. The expression of NF- 魏 Bp50 is increased with the increase of histological grade and TNM stage, but it is associated with the age of the patients. The difference of tumor size and molecular type was not statistically significant, suggesting that they were closely related to the degree of malignancy of breast cancer, and might be involved in promoting the progression of breast cancer. 3 the relationship between the level of NF- 魏 Bp50 mRNA in FAT10 and clinicopathological factors was not statistically significant. The expression of NF-xBp50 and NF-xBp50 in invasive ductal carcinoma of breast is positively correlated, suggesting that they may play a synergistic role in the occurrence and development of breast cancer.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.9

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