長鏈非編碼RNA-PRSS3P2在甲狀腺乳頭狀癌中的表達(dá)及功能研究
發(fā)布時(shí)間：2018-10-18 15:17
【摘要】：目的:探討絲氨酸蛋白酶3假基因2(PRSS3P2)與其真基因絲氨酸蛋白酶3(PRSS3)在甲狀腺乳頭狀癌(papillary thyroid carcinoma,PTC)中的表達(dá)差異及特點(diǎn),分析二者在PTC中表達(dá)的臨床病理意義;探討lnc RNA-PRSS3P2對(duì)甲狀腺乳頭狀癌TPC-1細(xì)胞的生物學(xué)功能的影響。方法:1.實(shí)時(shí)熒光定量RT-PCR方法檢測55例甲狀腺乳頭狀癌組織及癌旁組織中PRSS3-m RNA及l(fā)nc RNA-PRSS3P2的表達(dá)情況,分析PRSS3和PRSS3P2表達(dá)的相關(guān)性以及其與臨床病理特征之間關(guān)系。2.構(gòu)建PRSS3P2-sh RNA慢病毒載體,轉(zhuǎn)染TPC-1細(xì)胞,采用實(shí)時(shí)熒光定量RT-PCR方法檢測轉(zhuǎn)染前后TPC-1細(xì)胞中PRSS3P2-lnc RNA表達(dá)情況;CCK-8實(shí)驗(yàn)檢測細(xì)胞的增殖情況變化。3.免疫組織化學(xué)法檢測PRSS3蛋白在55例甲狀腺乳頭狀癌組織中的表達(dá),分析PRSS3蛋白在甲狀腺乳頭狀癌組織中表達(dá)情況及其與臨床病理學(xué)特征的關(guān)系。結(jié)果:1.55對(duì)標(biāo)本中,PRSS3-m RNA在PTC組中的表達(dá)水平明顯高于癌旁非腫瘤組織組的表達(dá)水平(7.20±1.87 vs.3.69±1.36),lnc RNA-PRSS3P2在PTC組中的表達(dá)水平也高于非腫瘤組織組(7.25±1.25 vs.3.53±1.06),二者的表達(dá)差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。在49例PTC組織中PRSS3表達(dá)高于癌旁組織,在47例PTC中PRSS3P2表達(dá)高于癌旁組織,其中42例PTC組織中的PRSS3和PRSS3P2的表達(dá)均高于癌旁組織。PTC患者PRSS3-m RNA相對(duì)表達(dá)量與患者性別、年齡、瘤體直徑、淋巴結(jié)轉(zhuǎn)移等臨床病理特征無明顯相關(guān)性;PTC患者lnc RNA-PRSS3P2相對(duì)表達(dá)量在不同年齡組的PTC患者中表達(dá)存在差別,其差異具有統(tǒng)計(jì)學(xué)意義(P0.05),而與其它臨床病理特征無明顯相關(guān)性。2.TPC-1細(xì)胞轉(zhuǎn)染PRSS3P2-sh RNA慢病毒表達(dá)載體后,PRSS3P2在PRSS3P2-sh RNA轉(zhuǎn)染組中的表達(dá)量(3.17±0.736)明顯低于空白對(duì)照組(7.92±0.583)和陰性對(duì)照組(7.48±0.894),其表達(dá)差異均具有統(tǒng)計(jì)學(xué)意義(P0.05),且空白對(duì)照組和陰性對(duì)照組的表達(dá)水平之間的差異無統(tǒng)計(jì)學(xué)意義;PRSS3P2在PRSS3P2-sh RNA轉(zhuǎn)染組中TPC-1細(xì)胞的增殖率從第2d開始低于對(duì)照組,但其差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3.PRSS3蛋白主要表達(dá)于甲狀腺乳頭狀癌細(xì)胞的胞漿中,鏡下呈棕黃色顆粒。PRSS3蛋白在大部分甲狀腺乳頭狀癌組織中有表達(dá)且表達(dá)的程度有所不同,陽性率為74.55%(41/55);而在癌旁非腫瘤組織中只有2例陽性表達(dá)(在相應(yīng)癌組織中也呈陽性表達(dá)),其它均為陰性;其中14例在甲狀腺乳頭狀癌及癌旁非腫瘤組織中都為陰性PRSS3蛋白主要表達(dá)于甲狀腺細(xì)胞胞漿。在55對(duì)標(biāo)本中,PRSS3蛋白在PTC組織中表達(dá)率明顯高于癌旁非腫瘤組織,且其不同表達(dá)程度與患者是否存在淋巴結(jié)轉(zhuǎn)移相關(guān)(P0.05),而與性別、年齡、瘤體直徑、包膜侵犯等其他臨床病理特征無明顯相關(guān)性(P0.05)。結(jié)論:PRSS3-m RNA和lnc RNA-PRSS3P2在PTC中的表達(dá)水平均明顯升高,這提示二者可能參與了PTC的發(fā)病過程;PRSS3-m RNA和lnc RNA-PRSS3P2可以作為PTC的診斷標(biāo)記物進(jìn)行進(jìn)一步研究。
[Abstract]:Objective: to investigate the expression of serine protease 3 (PRSS3P2) and its true gene serine protease 3 (PRSS3) in thyroid papillary carcinoma (papillary thyroid carcinoma,PTC), and to analyze the clinicopathological significance of the expression of serine protease 3 (PRSS3P2) and serine protease 3 (PRSS3) in (papillary thyroid carcinoma,PTC. To investigate the effect of lnc RNA-PRSS3P2 on the biological function of TPC-1 cells in papillary thyroid carcinoma. Methods: 1. Real-time fluorescence quantitative RT-PCR was used to detect the expression of PRSS3-m RNA and lnc RNA-PRSS3P2 in 55 cases of papillary thyroid carcinoma and its adjacent tissues. The correlation between the expression of PRSS3 and PRSS3P2 and the relationship between the expression of PRSS3 and the clinicopathological features were analyzed. 2. PRSS3P2-sh RNA lentivirus vector was constructed and transfected into TPC-1 cells. The expression of PRSS3P2-lnc RNA in TPC-1 cells before and after transfection was detected by real-time fluorescence quantitative RT-PCR, and the proliferation of TPC-1 cells was detected by CCK-8 assay. 3. Immunohistochemical method was used to detect the expression of PRSS3 protein in 55 cases of thyroid papillary carcinoma. The expression of PRSS3 protein in papillary thyroid carcinoma and its relationship with clinicopathological features were analyzed. Results: the expression of PRSS3-m RNA in PTC group was significantly higher than that in non-tumor tissue group (7.20 鹵1.87 vs.3.69 鹵1.36), lnc RNA-PRSS3P2, 7.20 鹵1.87 vs.3.69 鹵1.36), lnc RNA-PRSS3P2, 7.25 鹵1.25 vs.3.53 鹵1.06), and the difference was statistically significant (P0.05). In 49 cases of PTC, the expression of PRSS3 was higher than that of paracancerous tissues, and the expression of PRSS3P2 in 47 cases of PTC was higher than that of paracancerous tissues. The expression of PRSS3 and PRSS3P2 in 42 cases of PTC was higher than that of paracancerous tissues. The relative expression of PRSS3-m RNA in PTC patients was related to the sex and age of the patients. There was no significant correlation between the clinicopathological features such as tumor diameter, lymph node metastasis, and the relative expression of lnc RNA-PRSS3P2 in patients with PTC was different in PTC patients of different age groups. The difference was statistically significant (P0.05), but not correlated with other clinicopathological features. After transfection of PRSS3P2-sh RNA lentivirus expression vector by 2.TPC-1 cells, the expression of PRSS3P2 in PRSS3P2-sh RNA transfection group (3.17 鹵0.736) was significantly lower than that in blank control group (7.92 鹵0.583) and negative control group (7.92 鹵0.583). There was no significant difference in the expression of PRSS3P2 between the blank control group and the negative control group (P 0.05), and the proliferation rate of TPC-1 cells in the PRSS3P2-sh RNA transfection group was lower than that in the control group from the second day after transfection, and there was no significant difference in the expression level between the blank control group and the negative control group. But the difference was not statistically significant (P0.05). 3.PRSS3 protein was mainly expressed in the cytoplasm of papillary thyroid carcinoma cells, and was brown granules under microscope. PRSS3 protein was expressed in most papillary thyroid carcinoma tissues and the degree of expression was different. The positive rate was 74.55% (41 / 55), but only 2 cases were positive in the adjacent non-tumor tissues (also positive in the corresponding cancer tissues), and all the others were negative. In 14 cases of thyroid papillary carcinoma and adjacent non-tumor tissues negative PRSS3 protein was mainly expressed in the cytoplasm of thyroid cells. In 55 pairs of specimens, the expression rate of PRSS3 protein in PTC tissues was significantly higher than that in non-tumor tissues adjacent to cancer, and the different expression levels were related to the presence of lymph node metastasis (P0.05), but related to sex, age, tumor diameter, and tumor size. Other clinicopathological features such as capsule invasion had no significant correlation (P0.05). Conclusion: the expression of PRSS3-m RNA and lnc RNA-PRSS3P2 in PTC is significantly increased, which suggests that both of them may be involved in the pathogenesis of PTC, and PRSS3-m RNA and lnc RNA-PRSS3P2 can be used as diagnostic markers of PTC for further study.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R736.1
本文編號(hào)：2279524
[Abstract]:Objective: to investigate the expression of serine protease 3 (PRSS3P2) and its true gene serine protease 3 (PRSS3) in thyroid papillary carcinoma (papillary thyroid carcinoma,PTC), and to analyze the clinicopathological significance of the expression of serine protease 3 (PRSS3P2) and serine protease 3 (PRSS3) in (papillary thyroid carcinoma,PTC. To investigate the effect of lnc RNA-PRSS3P2 on the biological function of TPC-1 cells in papillary thyroid carcinoma. Methods: 1. Real-time fluorescence quantitative RT-PCR was used to detect the expression of PRSS3-m RNA and lnc RNA-PRSS3P2 in 55 cases of papillary thyroid carcinoma and its adjacent tissues. The correlation between the expression of PRSS3 and PRSS3P2 and the relationship between the expression of PRSS3 and the clinicopathological features were analyzed. 2. PRSS3P2-sh RNA lentivirus vector was constructed and transfected into TPC-1 cells. The expression of PRSS3P2-lnc RNA in TPC-1 cells before and after transfection was detected by real-time fluorescence quantitative RT-PCR, and the proliferation of TPC-1 cells was detected by CCK-8 assay. 3. Immunohistochemical method was used to detect the expression of PRSS3 protein in 55 cases of thyroid papillary carcinoma. The expression of PRSS3 protein in papillary thyroid carcinoma and its relationship with clinicopathological features were analyzed. Results: the expression of PRSS3-m RNA in PTC group was significantly higher than that in non-tumor tissue group (7.20 鹵1.87 vs.3.69 鹵1.36), lnc RNA-PRSS3P2, 7.20 鹵1.87 vs.3.69 鹵1.36), lnc RNA-PRSS3P2, 7.25 鹵1.25 vs.3.53 鹵1.06), and the difference was statistically significant (P0.05). In 49 cases of PTC, the expression of PRSS3 was higher than that of paracancerous tissues, and the expression of PRSS3P2 in 47 cases of PTC was higher than that of paracancerous tissues. The expression of PRSS3 and PRSS3P2 in 42 cases of PTC was higher than that of paracancerous tissues. The relative expression of PRSS3-m RNA in PTC patients was related to the sex and age of the patients. There was no significant correlation between the clinicopathological features such as tumor diameter, lymph node metastasis, and the relative expression of lnc RNA-PRSS3P2 in patients with PTC was different in PTC patients of different age groups. The difference was statistically significant (P0.05), but not correlated with other clinicopathological features. After transfection of PRSS3P2-sh RNA lentivirus expression vector by 2.TPC-1 cells, the expression of PRSS3P2 in PRSS3P2-sh RNA transfection group (3.17 鹵0.736) was significantly lower than that in blank control group (7.92 鹵0.583) and negative control group (7.92 鹵0.583). There was no significant difference in the expression of PRSS3P2 between the blank control group and the negative control group (P 0.05), and the proliferation rate of TPC-1 cells in the PRSS3P2-sh RNA transfection group was lower than that in the control group from the second day after transfection, and there was no significant difference in the expression level between the blank control group and the negative control group. But the difference was not statistically significant (P0.05). 3.PRSS3 protein was mainly expressed in the cytoplasm of papillary thyroid carcinoma cells, and was brown granules under microscope. PRSS3 protein was expressed in most papillary thyroid carcinoma tissues and the degree of expression was different. The positive rate was 74.55% (41 / 55), but only 2 cases were positive in the adjacent non-tumor tissues (also positive in the corresponding cancer tissues), and all the others were negative. In 14 cases of thyroid papillary carcinoma and adjacent non-tumor tissues negative PRSS3 protein was mainly expressed in the cytoplasm of thyroid cells. In 55 pairs of specimens, the expression rate of PRSS3 protein in PTC tissues was significantly higher than that in non-tumor tissues adjacent to cancer, and the different expression levels were related to the presence of lymph node metastasis (P0.05), but related to sex, age, tumor diameter, and tumor size. Other clinicopathological features such as capsule invasion had no significant correlation (P0.05). Conclusion: the expression of PRSS3-m RNA and lnc RNA-PRSS3P2 in PTC is significantly increased, which suggests that both of them may be involved in the pathogenesis of PTC, and PRSS3-m RNA and lnc RNA-PRSS3P2 can be used as diagnostic markers of PTC for further study.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R736.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前6條
1 劉璐;劉福囝;;lncRNA與腫瘤浸潤轉(zhuǎn)移的關(guān)系[J];解剖科學(xué)進(jìn)展;2013年04期
2 龔艷萍;龔日祥;楊敬;朱精強(qiáng);魏濤;李志輝;;甲狀腺乳頭狀癌BRAF基因突變與臨床病理特征間的關(guān)系及臨床意義[J];四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2013年04期
3 張敏;王銀萍;倪勁松;薛世泉;鄒亞彬;張麗紅;;CFTR及SPINK1蛋白在慢性胰腺炎及胰腺癌中的表達(dá)及其意義[J];中國實(shí)驗(yàn)診斷學(xué);2011年10期
4 郝儒田;張?bào)泸?潘貽飛;;甲狀腺乳頭狀癌與甲狀腺結(jié)節(jié)鈣化的關(guān)系探討[J];中國腫瘤臨床;2007年20期
5 方海飛;沈美萍;徐銀峰;;甲狀腺乳頭狀癌TGF-β_1、CD_(31)表達(dá)與腫瘤轉(zhuǎn)移的關(guān)系[J];南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版);2007年05期
6 謝榜昆,關(guān)玉寶,袁小平,李海剛;甲狀腺癌的CT表現(xiàn)與病理相關(guān)性研究[J];癌癥;2003年02期
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