【摘要】：目的:研究Notch2和Notch3在非小細(xì)胞肺癌(NSCLC)中的表達(dá),以及它們和相關(guān)臨床參數(shù)(包括性別、年齡、組織類(lèi)型、TNM分期、腫瘤分化程度、淋巴結(jié)轉(zhuǎn)移、原發(fā)灶大小、原發(fā)腫瘤狀態(tài))之間的聯(lián)系,從而探討Notch2和Notch3在非小細(xì)胞肺癌中表達(dá)的臨床意義,探尋能用于估測(cè)NSCLC病人生存預(yù)后的新型分子標(biāo)記物,以及NSCLC靶向治療的新靶點(diǎn)。方法:挑選手術(shù)后病理證實(shí)的非小細(xì)胞肺癌病人癌組織標(biāo)本60例,并且取30例癌旁正常肺組織當(dāng)作對(duì)照。通過(guò)制作石蠟組織切片、常規(guī)HE染色、免疫組織化學(xué)染色,運(yùn)用免疫組化方法,觀察Notch2、Notch3在非小細(xì)胞肺癌組織和癌旁正常肺組織中的表達(dá)情況。結(jié)果:1.Notch2主要表達(dá)于腫瘤細(xì)胞的細(xì)胞質(zhì),Notch2在非小細(xì)胞肺癌組織中的表達(dá)水平大大高于癌旁肺組織(P0.05);Notch2表達(dá)與非小細(xì)胞肺癌病人的性別、年齡、組織類(lèi)型及原發(fā)灶大小無(wú)明顯相關(guān)性(P0.05);Notch2陽(yáng)性表達(dá)率在Ⅲ、Ⅳ期NSCLC患者中明顯高于Ⅰ、Ⅱ期患者(P0.05),低分化患者顯然高于高分化患者(P0.05),有淋巴結(jié)轉(zhuǎn)移患者明顯高于無(wú)淋巴結(jié)轉(zhuǎn)移患者(P0.05),原發(fā)腫瘤T3、T4期患者明顯高于T1、T2期患者(P0.05);2.Notch3亦主要表達(dá)于細(xì)胞質(zhì),Notch3在非小細(xì)胞肺癌組織中的表達(dá)率大大高于癌旁肺組織,其陽(yáng)性表達(dá)率在Ⅲ、Ⅳ期NSCLC患者中明顯高于Ⅰ、Ⅱ期患者,低分化患者顯然高于高分化患者,有淋巴結(jié)轉(zhuǎn)移患者明顯高于無(wú)淋巴結(jié)轉(zhuǎn)移患者,原發(fā)腫瘤T3、T4期患者明顯高于T1、T2期患者,上述差異均具有統(tǒng)計(jì)學(xué)意義(P0.05);Notch3的表達(dá)與非小細(xì)胞肺癌病人的性別、年齡、組織類(lèi)型及原發(fā)灶大小也無(wú)明顯相關(guān)性(P0.05);3.Notch2、Notch3在NSCLC中的表達(dá)呈正相關(guān)(P0.05)。結(jié)論:Notch2和Notch3在非小細(xì)胞肺癌組織中異常表達(dá)升高,Notch2和Notch3的表達(dá)與非小細(xì)胞肺癌臨床分期、腫瘤分化程度、原發(fā)腫瘤狀態(tài)、有無(wú)淋巴結(jié)轉(zhuǎn)移緊密相關(guān),在非小細(xì)胞肺癌的發(fā)生發(fā)展中發(fā)揮作用,在評(píng)估非小細(xì)胞肺癌病人的生存預(yù)后方面有一定的參考和指導(dǎo)意義。
[Abstract]:Objective: to investigate the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) (NSCLC), as well as their clinical parameters (including sex, age, tissue type, TNM stage, tumor differentiation, lymph node metastasis, primary tumor size). To explore the clinical significance of the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) and to explore a new molecular marker for estimating the survival and prognosis of NSCLC patients and a new target for NSCLC targeted therapy. Methods: 60 patients with non-small cell lung cancer and 30 normal adjacent lung tissues were selected as control group. The expression of Notch2,Notch3 in non-small cell lung cancer (NSCLC) and adjacent normal lung tissues (NSCLC) was observed by paraffin section, routine HE staining and immunohistochemical staining. Results: 1.Notch2 was mainly expressed in the cytoplasm of tumor cells. The expression of Notch2 in NSCLC was significantly higher than that in adjacent lung tissues (P0.05), and the expression of Notch2 was correlated with the sex and age of NSCLC patients. There was no significant correlation between the histological type and the size of the primary tumor (P0.05), and the positive expression rate of Notch2 was significantly higher in the patients with stage 鈪，

本文編號(hào)：2271988