寧夏地區(qū)鼻咽癌與EBV和HPV的相關(guān)性研究
發(fā)布時間：2018-10-09 19:37
【摘要】：目的:研究寧夏鼻咽癌患者組織中EB病毒(Epstein-Barr virus,EBV)與人乳頭狀瘤病毒(Human Papillomavirus,HPV)的感染情況及HPV基因型分布情況,并與南方鼻咽癌患者作比較,為探討我區(qū)鼻咽癌預(yù)防、篩查、早期診斷和治療、預(yù)后評價等提供科學依據(jù)。方法:收集2011年~2016年在寧夏醫(yī)科大學附屬總醫(yī)院和上海公利醫(yī)院經(jīng)活檢病理檢查確診的107例鼻咽癌患者石蠟組織樣本采用核酸分子快速導(dǎo)流雜交基因芯片分型技術(shù)(HybriMax)和實時熒光定量PCR技術(shù)進行EBV定量檢測和37種HPV亞型檢測,并以95例鼻咽部黏膜組織慢性炎癥患者的石蠟組織為對照組。結(jié)果:寧夏地區(qū)鼻咽癌患者與鼻咽部黏膜組織慢性炎癥患者組織中EBV的總感染率分別是96.22%(51/53)、34.09%(15/44)上海地區(qū)鼻咽癌患者與鼻咽部黏膜組織慢性炎癥患者組織中EBV的總感染率分別是85.2%(46/54)、17.6%(9/51),EBV感染在兩地區(qū)鼻咽癌和鼻咽部黏膜組織慢性炎癥患者組織中的表達差異有統(tǒng)計學意義(P0.05),EBV感染在寧夏與上海鼻咽癌患者組織中的表達差異無統(tǒng)計學意義(P0.05)。寧夏地區(qū)鼻咽癌患者與鼻咽部黏膜組織慢性炎癥患者組織中HPV總感染性率為分別是45.2%(24/53)、9%(4/44)HPV感染總陽性率在鼻咽癌和鼻咽部黏膜組織慢性炎癥組織中的表達差異有統(tǒng)計學意義(P0.05),其中單一類型感染最常見的為HPV16+45.83%(11/24),其次為HPV71+9.16%(7/24),EBV和HPV兩種病毒混合感染在寧夏鼻咽癌和鼻咽部黏膜組織慢性炎癥患者組織中總感染率分別為45.3%(24/53)和4.5%(2/44),HPV與EBV兩種病毒混合感染在寧夏鼻咽癌和鼻咽部黏膜組織慢性炎癥患者組織中的表達差異有統(tǒng)計學意義(P0.05)寧夏地區(qū)鼻咽癌患者組織中EBV感染與年齡、性別、民族、病理類型、臨床分期無明顯相關(guān)性,差異無統(tǒng)計學意義(P0.05)。結(jié)論:寧夏地區(qū)鼻咽癌發(fā)生發(fā)展與EBV和HPV感染密切相關(guān),最主要以EBV感染為主,有一定數(shù)量的HPV感染,HPV感染亞型中以單一感染HPV16+型最常見,同時有大約50%的鼻咽癌患者存在EBV和HPV混合感染。寧夏地區(qū)鼻咽癌患者組織中EBV感染與年齡、性別、民族、病理類型、臨床分期無明顯相關(guān)性。上海地區(qū)鼻咽癌發(fā)生、發(fā)展主要與EBV感染相關(guān),有極少量的HPV感染,EBV感染在寧夏地區(qū)和上海地區(qū)鼻咽癌患者組織中無明顯差異
[Abstract]:Objective: to study the infection of EB virus (Epstein-Barr virus,EBV) and human papillomavirus (Human Papillomavirus,HPV) and the distribution of HPV genotypes in patients with nasopharyngeal carcinoma (NPC) in Ningxia. Early diagnosis and treatment, prognosis evaluation and so on provides the scientific basis. Methods: from 2011 to 2016, 107 paraffin tissue samples of nasopharyngeal carcinoma confirmed by biopsy and pathological examination were collected from affiliated General Hospital of Ningxia Medical University and Shanghai Gongli Hospital. (HybriMax) and real-time fluorescence quantitative PCR were used to detect EBV and 37 HPV subtypes. The paraffin tissues of 95 patients with chronic inflammation of nasopharyngeal mucosa were taken as control group. Results: the total infection rate of EBV was 96.22% (51 / 53) and 34.09% (15 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa, respectively. The total infection rate of EBV in patients with nasopharyngeal carcinoma and patients with chronic inflammation of nasopharyngeal mucosa in Shanghai was 96.22% (51 / 53) and 34.09% (15 / 44) respectively. The rates of EBV infection were 85.2% (46 / 54) and 17.6% (9 / 51) respectively. There was significant difference in the expression of EBV infection in patients with chronic inflammation of nasopharyngeal carcinoma and nasopharyngeal mucosa in the two regions (P0.05). There was no significant difference in the expression of EBV infection between Ningxia and Shanghai (P0.05). The total infection rate of HPV was 45.2% (24 / 53) or 9% (4 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa in Ningxia area. The most common type of infection was HPV16 45.83% (11 / 24), followed by HPV71 9.16% (7 / 24). The total infection rates of HPV71 and HPV were 45.3% (24 / 53) and 4.5% (2 / 44) in patients with chronic inflammation of nasopharyngeal mucosa in Ningxia. There was significant difference in the expression of EBV between nasopharyngeal carcinoma and nasopharyngeal mucosa in patients with chronic inflammation of nasopharyngeal carcinoma (P0.05). Gender, nationality, pathological type, clinical stage had no significant correlation, the difference was not statistically significant (P0.05). Conclusion: the occurrence and development of nasopharyngeal carcinoma in Ningxia area are closely related to EBV and HPV infection, the main infection is EBV infection, and the single infection HPV16 is the most common subtype of HPV infection. At the same time, about 50% of NPC patients have mixed infection of EBV and HPV. There was no significant correlation between EBV infection and age, sex, nationality, pathological type and clinical stage in patients with nasopharyngeal carcinoma in Ningxia. The occurrence and development of nasopharyngeal carcinoma in Shanghai area is mainly related to EBV infection. There is no significant difference in HPV infection between Ningxia region and Shanghai area.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.63
[Abstract]:Objective: to study the infection of EB virus (Epstein-Barr virus,EBV) and human papillomavirus (Human Papillomavirus,HPV) and the distribution of HPV genotypes in patients with nasopharyngeal carcinoma (NPC) in Ningxia. Early diagnosis and treatment, prognosis evaluation and so on provides the scientific basis. Methods: from 2011 to 2016, 107 paraffin tissue samples of nasopharyngeal carcinoma confirmed by biopsy and pathological examination were collected from affiliated General Hospital of Ningxia Medical University and Shanghai Gongli Hospital. (HybriMax) and real-time fluorescence quantitative PCR were used to detect EBV and 37 HPV subtypes. The paraffin tissues of 95 patients with chronic inflammation of nasopharyngeal mucosa were taken as control group. Results: the total infection rate of EBV was 96.22% (51 / 53) and 34.09% (15 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa, respectively. The total infection rate of EBV in patients with nasopharyngeal carcinoma and patients with chronic inflammation of nasopharyngeal mucosa in Shanghai was 96.22% (51 / 53) and 34.09% (15 / 44) respectively. The rates of EBV infection were 85.2% (46 / 54) and 17.6% (9 / 51) respectively. There was significant difference in the expression of EBV infection in patients with chronic inflammation of nasopharyngeal carcinoma and nasopharyngeal mucosa in the two regions (P0.05). There was no significant difference in the expression of EBV infection between Ningxia and Shanghai (P0.05). The total infection rate of HPV was 45.2% (24 / 53) or 9% (4 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa in Ningxia area. The most common type of infection was HPV16 45.83% (11 / 24), followed by HPV71 9.16% (7 / 24). The total infection rates of HPV71 and HPV were 45.3% (24 / 53) and 4.5% (2 / 44) in patients with chronic inflammation of nasopharyngeal mucosa in Ningxia. There was significant difference in the expression of EBV between nasopharyngeal carcinoma and nasopharyngeal mucosa in patients with chronic inflammation of nasopharyngeal carcinoma (P0.05). Gender, nationality, pathological type, clinical stage had no significant correlation, the difference was not statistically significant (P0.05). Conclusion: the occurrence and development of nasopharyngeal carcinoma in Ningxia area are closely related to EBV and HPV infection, the main infection is EBV infection, and the single infection HPV16 is the most common subtype of HPV infection. At the same time, about 50% of NPC patients have mixed infection of EBV and HPV. There was no significant correlation between EBV infection and age, sex, nationality, pathological type and clinical stage in patients with nasopharyngeal carcinoma in Ningxia. The occurrence and development of nasopharyngeal carcinoma in Shanghai area is mainly related to EBV infection. There is no significant difference in HPV infection between Ningxia region and Shanghai area.
【學位授予單位】：寧夏醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.63
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