發(fā)布時間：2018-10-05 20:25

【摘要】：目的腫瘤源性的血管性血友病因子(von Willbrend factor,vWF)表達及血管生成擬態(tài)(vasculogenic mimicry,VM)形成都是高度惡性腫瘤中的高頻事件。本研究檢測甲狀腺未分化癌組織中vWF的表達及VM水平,在組織學水平上探討vWF的表達及其與腫瘤組織中VM形成的相關性。方法選取甘肅省人民醫(yī)院(20例)及甘肅省腫瘤醫(yī)院(43例)2000-01-01-2014-12-31病理診斷為甲狀腺未分化癌的手術標本63例。同時隨機挑選甘肅省人民醫(yī)院同期甲狀腺乳頭狀癌病例63例和相應癌旁正常組織作為對照。采用免疫組化法檢測甲狀腺組織中vWF蛋白的表達水平,CD31聯(lián)合PAS雙重染色檢測VM水平。結果vWF蛋白在正常甲狀腺組織無表達,甲狀腺乳頭狀癌中有5例(7.90%)陽性表達,甲狀腺未分化癌中vWF陽性表達31例(49.20%),組間比較差異有統(tǒng)計學意義,χ~2=26.289,P0.001。vWF在甲狀腺未分化癌中的陽性表達與患者的性別、年齡、腫瘤大小和淋巴結轉移無關,而與遠處轉移(χ~2=5.341,P=0.021)和臨床分期(χ~2=4.753,P=0.029)相關。VM在正常甲狀腺組織、乳頭狀癌及甲狀腺未分化癌中的形成率分別為0、3.20%和34.92%,組間差異有統(tǒng)計學意義,χ~2=20.588,P0.001。VM形成與腫瘤最大徑(χ~2=4.789,P=0.029)、淋巴結轉移(χ~2=5.684,P=0.028)、遠處轉移(χ~2=4.722,P=0.030)和臨床分期(χ~2=5.777,P=0.061)相關。vWF的表達與VM水平正相關,r=0.411,P=0.001。結論甲狀腺未分化癌中有不同程度vWF的高表達,其高表達與VM的形成正相關,vWF的高表達可能是導致甲狀腺未分化癌預后差的因素之一。
[Abstract]:Objective the expression of tumor derived von Willebrand factor (von Willbrend factor,vWF) and the formation of angiogenic mimicry (vasculogenic mimicry,VM) are high frequency events in highly malignant tumors. In this study, the expression of vWF and the level of VM were detected in undifferentiated thyroid carcinoma. The expression of vWF and its correlation with the formation of VM in tumor tissue were studied at the histological level. Methods Sixty-three cases of undifferentiated thyroid carcinoma were selected from Gansu Provincial people's Hospital (20 cases) and Gansu Cancer Hospital (43 cases) from January to January 2014-12-31, 2000. At the same time, 63 cases of thyroid papillary carcinoma in Gansu Provincial people's Hospital were randomly selected as control group. The expression of vWF protein in thyroid tissue was detected by immunohistochemical method and the VM level was detected by double staining of CD31 and PAS. Results there was no expression of vWF protein in normal thyroid tissue, and 5 cases (7.90%) were positive in papillary thyroid carcinoma. 31 cases (49.20%) of thyroid undifferentiated carcinoma showed vWF positive expression. The positive expression of vWF in undifferentiated thyroid carcinoma was not correlated with sex, age, tumor size and lymph node metastasis. VM was associated with distant metastasis (蠂 2 + 5.341) and clinical stage (蠂 2 / 24.753) in normal thyroid tissue. The formation rates of papillary carcinoma and thyroid undifferentiated carcinoma were 0 / 3.20% and 34.92%, respectively. There was significant difference between the two groups. The expression of VWF was positively correlated with the VM level and the maximum diameter of the tumor (蠂 2 / 24.789 / P 0.029), lymph node metastasis (蠂 ~ 2 / 25.684P / 0.028), distant metastasis (蠂 ~ 2 / 2 / 4.722 / P ~ 0.030) and clinical stage (蠂 ~ 25.777A / P 0.061). The expression of VWF was positively correlated with the VM level. Conclusion there is high expression of vWF in undifferentiated thyroid carcinoma. The high expression of vWF and the formation of VM may be one of the factors leading to poor prognosis of undifferentiated thyroid carcinoma.
【作者單位】： 甘肅省人民醫(yī)院病理科;甘肅省腫瘤醫(yī)院病理科;
【分類號】：R736.1

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