【摘要】：目的:探討載阿霉素介孔二氧化硅納米粒(MSNs@DOX)對(duì)體外培養(yǎng)的人舌癌Tca-8113細(xì)胞增殖及凋亡的作用。方法:透射電鏡(TEM)和氮吸附法對(duì)介孔二氧化硅納米粒(MSNs)進(jìn)行表征;紫外分光光度計(jì)檢測(cè)阿霉素(DOX)釋放行為;MTT法檢測(cè)細(xì)胞增殖抑制作用;Hochest33342染色、流式細(xì)胞術(shù)檢測(cè)細(xì)胞凋亡和周期分布;Western blot檢測(cè)P53、Bcl-2、Bax和Caspase-3蛋白表達(dá)。結(jié)果:MSNs形態(tài)規(guī)則,分布均一,平均粒徑、介孔孔徑、比表面積和孔容分別為約30nm,19.59682nm、193.4296m2/g、0.947625cm3/g;MSNs細(xì)胞相容性良好;與單獨(dú)DOX相比,載藥組的細(xì)胞增殖抑制率及凋亡率更顯著,且呈一定MSNs濃度依賴(lài)性(P0.05);細(xì)胞明顯阻滯于G0/G1期;P53、Bax、Caspase-3表達(dá)顯著升高,Bcl-2表達(dá)顯著降低。結(jié)論:該載藥納米傳輸系統(tǒng)有望用于舌癌治療。
[Abstract]:Aim: to investigate the effect of doxorubicin loaded mesoporous silica nanoparticles (MSNs@DOX) on proliferation and apoptosis of human tongue cancer Tca-8113 cells in vitro. Methods: the (MSNs) of mesoporous silica nanoparticles was characterized by transmission electron microscopy (TEM) and nitrogen adsorption, and the release behavior of doxorubicin (DOX) was detected by UV spectrophotometer. Apoptosis and cell cycle distribution were detected by flow cytometry. Western blot was used to detect the expression of Bcl-2 Bax and Caspase-3 protein. Results the morphology, distribution, mean particle size, mesoporous pore size, specific surface area and pore volume were about 30 nm ~ (-1), 19.59682 nm ~ (-1), 193.4296 m ~ (2 /) 路g ~ (-1) ~ 0. 47625 cm ~ (3) / g ~ (-1), respectively. Compared with DOX alone, the cell proliferation inhibition rate and apoptosis rate of drug loaded group were more significant. The expression of Caspase-3 was significantly increased in the G0/G1 phase and the expression of Bcl-2 was significantly decreased. Conclusion: the drug-loaded nano-delivery system is expected to be used in the treatment of tongue cancer.
【作者單位】： 蘭州大學(xué)口腔醫(yī)學(xué)院口腔修復(fù)科;中國(guó)科學(xué)院蘭州化學(xué)物理研究所固體潤(rùn)滑國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：中央高校自由探索-面上項(xiàng)目(編號(hào):lzujbky-2015-293) 甘肅省衛(wèi)生行業(yè)科研計(jì)劃管理項(xiàng)目(編號(hào):GWGL2014-06)
【分類(lèi)號(hào)】：R739.86

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