【摘要】：目的:Krüppel樣因子15(Krüppel-like factor 15,KLF15)參與腫瘤的增殖、侵襲和轉(zhuǎn)移,但在肺腺癌中的表達(dá)和作用尚不明確。本研究探索KLF15蛋白在肺腺癌患者中的表達(dá)及其臨床意義。方法:收集我院4例肺腺癌組織及匹配的癌旁組織,Western blot分析這些組織中KLF15蛋白表達(dá)的差異。同時(shí)收集我院72例肺腺癌患者的腫瘤組織標(biāo)本及臨床資料,免疫組織化學(xué)染色分析患者肺癌標(biāo)本中KLF15蛋白的表達(dá),分析其表達(dá)與患者臨床特征包括預(yù)后的關(guān)系。另外,采用質(zhì)粒轉(zhuǎn)染的方法上調(diào)肺腺癌A549細(xì)胞中KLF15的表達(dá),CCK-8法分析KLF15蛋白對(duì)肺腺癌細(xì)胞增殖能力的影響。結(jié)果:在4例肺腺癌及匹配的癌旁組織中,肺腺癌組織中KLF15蛋白表明顯低于癌旁組織。在72例肺腺癌病理標(biāo)本中,KLF15蛋白低表達(dá)或不表達(dá)者53例,占73.6%,KLF15高表達(dá)者19例,占26.4%。KLF15蛋白高表達(dá)的患者,其5年生存率明顯優(yōu)于KLF15低表達(dá)的患者(P0.01);KLF15蛋白表達(dá)與肺腺癌患者的病理分期(P0.01)及T分期明顯相關(guān)(P0.01),KLF15蛋白低表達(dá)是肺腺癌患者不良預(yù)后的重要危險(xiǎn)因素。上調(diào)肺腺癌細(xì)胞中KLF15蛋白的表達(dá)可明顯抑制肺腺癌細(xì)胞的生長。結(jié)論:KLF15抑制肺腺癌細(xì)胞的生長,有望成為肺腺癌患者的治療靶點(diǎn)和預(yù)后分子標(biāo)志物。
[Abstract]:Objective Kr 眉 Kr 眉 ppel like factor 15 (Kr 眉 ppel-like factor 15 KLF15) is involved in the proliferation, invasion and metastasis of tumor, but the expression and role of Kr 眉 Kr 眉 KLF15 in lung adenocarcinoma is unclear. This study was to investigate the expression and clinical significance of KLF15 protein in patients with lung adenocarcinoma. Methods: the expression of KLF15 protein in 4 cases of lung adenocarcinoma and matched adjacent tissues were analyzed by Western blot. At the same time, the tumor tissue and clinical data of 72 patients with lung adenocarcinoma in our hospital were collected. The expression of KLF15 protein was analyzed by immunohistochemical staining, and the relationship between the expression of KLF15 protein and the clinical features, including prognosis, was analyzed. In addition, the expression of KLF15 in lung adenocarcinoma A549 cells was up-regulated by plasmid transfection. The effect of KLF15 protein on the proliferation of lung adenocarcinoma A549 cells was analyzed by CCK-8 method. Results: in 4 cases of lung adenocarcinoma and matched paracancerous tissues, the expression of KLF15 protein in lung adenocarcinoma was significantly lower than that in paracancerous tissues. In 72 cases of lung adenocarcinoma, there were 53 cases with low or no expression of KLF15 protein, 19 cases with high expression of KLF15 protein in 73.6 cases, and 19 cases with high expression of 26.4%.KLF15 protein. The 5-year survival rate was significantly better than that in patients with low expression of KLF15 (P0.01). The expression of KLF15 protein was significantly correlated with pathological stage (P0.01) and T stage (P0.01) of patients with lung adenocarcinoma, which was an important risk factor for poor prognosis of patients with lung adenocarcinoma. Upregulation of KLF15 protein in lung adenocarcinoma cells significantly inhibited the growth of lung adenocarcinoma cells. Conclusion KLF15 inhibits the growth of lung adenocarcinoma cells and may be a therapeutic target and prognostic molecular marker in lung adenocarcinoma patients.
【作者單位】： 暨南大學(xué)附屬第一醫(yī)院呼吸內(nèi)科;
【基金】：國家自然科學(xué)基金青年基金資助項(xiàng)目(No.81302030)
【分類號(hào)】：R734.2

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本文編號(hào)：2231072