【摘要】：三磷酸腺苷結(jié)合盒蛋白E1(ABCE1)是ATP結(jié)合盒蛋白亞家族成員之一,在病毒感染、細(xì)胞增殖、抗凋亡、翻譯起始和核糖體生物發(fā)生等過(guò)程中發(fā)揮重要作用。為了探討ABCE1對(duì)神經(jīng)膠質(zhì)瘤細(xì)胞U251增殖、遷移和凋亡的作用,本研究通過(guò)實(shí)時(shí)熒光定量PCR和免疫印跡實(shí)驗(yàn),檢測(cè)ABCE1在神經(jīng)膠質(zhì)瘤細(xì)胞和正常膠質(zhì)細(xì)胞中的mRNA和蛋白質(zhì)表達(dá)水平。結(jié)果顯示,ABCE1在神經(jīng)膠質(zhì)瘤細(xì)胞U251中的表達(dá)高于在正常膠質(zhì)細(xì)胞中的表達(dá)。利用siRNA靶向沉默ABCE1后,在神經(jīng)膠質(zhì)瘤細(xì)胞U251中,ABCE1 mRNA和蛋白質(zhì)的表達(dá)水平均顯著減少,細(xì)胞的凋亡率顯著提高,細(xì)胞增殖和遷移明顯受到抑制,而且細(xì)胞對(duì)化療藥物替莫唑胺的敏感性增強(qiáng)。此外,沉默ABCE1后使Bcl-2的mRNA和蛋白質(zhì)表達(dá)水平顯著下調(diào),而B(niǎo)ax的mRNA和蛋白質(zhì)表達(dá)水平顯著上調(diào)。以上結(jié)果表明,ABCE1與神經(jīng)膠質(zhì)瘤細(xì)胞的增殖和遷移密切相關(guān),通過(guò)siRNA靶向沉默ABCE1基因,可顯著降低U251細(xì)胞的增殖和遷移能力。
[Abstract]:Adenosine triphosphate binding cassette protein E1 (ABCE1) is a member of ATP binding cassette protein subfamily, which plays an important role in virus infection, cell proliferation, anti-apoptosis, translation initiation and ribosomal biogenesis. In order to investigate the effect of ABCE1 on the proliferation, migration and apoptosis of glioma cell U251, the expression of mRNA and protein in glioma cells and normal glioma cells were detected by real-time quantitative PCR and Western blot. The results showed that the expression of ABCE1 in glioma cell U251 was higher than that in normal glioma cell. After silencing ABCE1 with siRNA, the expression of ABCE1 mRNA and protein in glioma cell U251 was significantly decreased, the apoptosis rate was significantly increased, and cell proliferation and migration were inhibited significantly. Moreover, cell sensitivity to the chemotherapeutic drug temozolamide was enhanced. In addition, after silencing ABCE1, the expression of mRNA and protein in Bcl-2 was significantly down-regulated, while that of mRNA and protein in Bax was upregulated. These results suggest that ABCE1 is closely related to the proliferation and migration of glioma cells, and the ability of proliferation and migration of U251 cells can be significantly reduced by silencing the ABCE1 gene by siRNA.
【作者單位】： 山西大學(xué)生物技術(shù)研究所化學(xué)生物學(xué)與分子工程教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(No.31372199) 山西省自然科學(xué)基金(No.201601D202055)資助項(xiàng)目~~
【分類號(hào)】：R739.41

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