【摘要】：目的:腫瘤內(nèi)部上皮間質(zhì)轉(zhuǎn)化和腫瘤干細(xì)胞的差異很少被研究。本研究的目的是研究上皮間質(zhì)轉(zhuǎn)化和腫瘤干細(xì)胞相關(guān)標(biāo)志在人胰腺導(dǎo)管腺癌的不同部位的表達(dá)狀態(tài)并探索他們和臨床病理特征的關(guān)聯(lián)。方法:胰腺導(dǎo)管腺癌瘤體被從中心到外周平均等分為3個(gè)部分,即核心、中間和外周。從20位經(jīng)歷手術(shù)患者的切除標(biāo)本中獲得胰腺導(dǎo)管腺癌的三個(gè)不同部位的癌組織和相對(duì)應(yīng)的癌旁組織。病理圖像分析的軟件Image Pro Plus 6.0被用來(lái)量化免疫組化染色結(jié)果。總光密度值IOD代表腫瘤干細(xì)胞標(biāo)志物CD44和上皮間質(zhì)轉(zhuǎn)化標(biāo)志物E-cadherin和N-cadherin的表達(dá)水平。胰腺導(dǎo)管腺癌的腫瘤干細(xì)胞和上皮間質(zhì)轉(zhuǎn)化標(biāo)志物的表達(dá)水平的臨床意義也被分析。結(jié)果:胰腺導(dǎo)管腺癌組織與相應(yīng)的癌旁組織中CD44,E-cadherin和N-cadherin的表達(dá)顯著不同(P0.05)。然而,胰腺導(dǎo)管腺癌核心,中部和外圍區(qū)域上皮間質(zhì)轉(zhuǎn)化和腫瘤干細(xì)胞標(biāo)志物無(wú)顯著差異(P0.05)。此外,CD44和N-cadherin的高表達(dá),以及E-cadherin低表達(dá)與淋巴結(jié)轉(zhuǎn)移和血管侵襲有關(guān)(P0.05)。CD44與N-cadherin 呈正相關(guān)(P0.05),E-cadherin 與 CD44 和 N-cadherin 呈負(fù)相關(guān)(P0.05)。結(jié)論:CD44和N-cadherin的高水平表達(dá)和E-cadherin的低水平表達(dá)和淋巴轉(zhuǎn)移和血管侵犯有關(guān)聯(lián)。再者,在胰腺導(dǎo)管腺癌,腫瘤干細(xì)胞特征和上皮間質(zhì)轉(zhuǎn)化程序密切相關(guān)。
[Abstract]:Objective: the difference between intraepithelial mesenchymal transformation and stem cells has rarely been studied. The purpose of this study was to investigate the expression of epithelial mesenchymal transformation and tumor stem cell related markers in different parts of human pancreatic ductal adenocarcinoma and to explore their association with clinicopathological features. Methods: pancreatic ductal adenocarcinoma was divided into three parts: core, middle and periphery. Three different tumor tissues and corresponding paracancerous tissues of pancreatic ductal adenocarcinoma were obtained from 20 surgical patients. The pathological image analysis software Image Pro Plus 6.0 was used to quantify the immunohistochemical staining results. Total optical density (IOD) represents the expression level of tumor stem cell marker CD44 and epithelial mesenchymal transformation marker E-cadherin and N-cadherin. The clinical significance of the expression of tumor stem cells and epithelial mesenchymal transformation markers in pancreatic ductal adenocarcinoma was also analyzed. Results: the expression of CD44,E-cadherin and N-cadherin in pancreatic ductal adenocarcinoma tissues was significantly different from that in corresponding paracancerous tissues (P0.05). However there was no significant difference in epithelial mesenchymal transformation and tumor stem cell markers in the core central and peripheral areas of pancreatic ductal adenocarcinoma (P0.05). In addition, the high expression of CD44 and N-cadherin, and the low expression of E-cadherin were related to lymph node metastasis and vascular invasion (P0.05). CD44 was positively correlated with N-cadherin (P0.05) E-cadherin was negatively correlated with CD44 and N-cadherin (P0.05). Conclusion the high expression of CD44 and N-cadherin is associated with the low expression of E-cadherin and lymphatic metastasis and vascular invasion. Furthermore, in pancreatic ductal adenocarcinoma, the characteristics of tumor stem cells are closely related to the epithelial interstitial transformation process.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.9

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